We narrowed to 655 results for: EPO

...scientist. New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...your own Fragmid component (which you can also deposit with Addgene!) We’re excited to help the Doench...

...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's what...2024 under a CC-BY 4.0 license.  The team deposited an array of constructs for tagging the kinesins...Hao and Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...Fluorescent Protein (GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's...FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric, at ...Incorporation into a FRET biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring...help you make better fluorescent fusion protein reporters for your studies and greatly lower your chances...

...expression plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific...including Protein A, 1xFLAG, 2xAM, and HA have been reported, as well as affinity purification with an anti-Cas9...chromatin immunoprecipitation (enChIP)." Scientific reports 3 (2013): 3171. PubMed PMID: 24201379. PubMed Central...Find CRISPR plasmids by function Find recently deposited plasmids ...

...are removed and replaced by a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked...necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase (reviewed...the minigenome as a template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase...start signal and initiates transcription of the reporter gene. The polymerase will elongate the mRNA until...

...technology more user-friendly and precise. We will deposit the plasmids of these systems to Addgene in the...Scientific Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's..., as well as the Editorial Board of Scientific Reports and PLoS One. Additionally, you are involved in...shopping for a wide range of plasmids. The merit of depositing plasmids to Addgene has been spectacular. We'...

...maintaining fluorescence. The protein structure, first reported in 1996, is an eleven β-sheet-containing “barrel...constructing fluorescent fusions. Transcription Reporter: Placing GFP under the control of a promoter of...a given cell type. This type of transcription reporting was among the earliest uses of GFP. Fluorescence...micrometastases in nude mice in cancer studies, act as a reporter for DNA double strand break repair, and to label...

...-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith Joung, PhD, and Feng Zhang...Center will continue to expand as Rett scientists deposit their MECP2-related plasmid tools with Addgene ...Researchers can also find links to cell line repositories including patient-derived iPSCs, MECP2 animal...Neurological Disorder by Programmable RNA Editing. Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878...

... extract as much information as possible from depositing laboratories and associated publications, but...would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while more recently...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...scale our lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing...