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  1. Tips for arabidopsis transformation

    Type
    Blog Post
    ... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...insertion likely precipitated domestication of sweet potatoes (Kyndt et al., 2015)! It sounds simple, and it.... et al. (2015) ‘The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes...
  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...up with poor droplet quality. Using a droplet generator, the sample and the droplet generation oil are...Pipette slowly If you are using a manual droplet generator, you will have to be careful about transferring...transferring your droplets from the droplet generator to the PCR plate. Maintaining a high droplet count is important...sample. Even if you are using an automated droplet generator, you should avoid pipetting too quickly when making...
  3. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...is the Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations...through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database...operations, she also works on improving laboratory efficiency through automation.       More Resource Avaliable...
  4. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...amount of grant funds available and the increasing regulatory process for grants has fostered the demand for...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...worked for a health department and then for the regulatory services department as our healthy homes program...
  5. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (Enabling Discovery ...parenting strategies and make important ecological indicators since species live in water and on land. Current...that tastes yucky (and is poisonous) for bird predators - birds eat a caterpillar once, vomit, and never... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...
  6. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post
    ...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...platforms to reliably detect nucleic acids at the atomolar level. The Zhang lab has adapted natural RNase...reporter enables SHERLOCK to detect DNA or RNA with atomolar sensitivity and single base pair mismatch specificity... of HPV, HPV16 and HPV18, from human cells, at atomolar levels, within one hour. DETECTR thus has the ...
  7. Hot Plasmids Spring 2024

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    Blog Post
    ... vector preps expressing the Voltron2 voltage indicator, created by the Schreiter Lab and the GENIE Project... available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they...The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute...
  8. The Challenges of Cell Culture

    Type
    Blog Post
    ...simple as a piece of paper on the front of the incubator containing the passage number, cell line, and ...book and on the actual flasks of cells in the incubator). This means any other scientist who wishes to...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...
  9. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...characterized genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from.... The Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554/elife.50651...
  10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...
Showing: 161 - 170 of 641 results