We narrowed to 990 results for: Abo

...name from Novophage to Sample 6. To learn more about Novophage's successful transformation into Sample...to focus on a technology that one is passionate about developing? 38:00 - End : Dr. Koeris' role on the...

...scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside of the...distribute their plasmids. The partnerships and collaborations with institutions and tech transfer offices...

...Of course, all of you toiling away in laboratories this holiday season want the work you are doing to... has to do with online accessibility. It is now about as easy to find a paper in a lesser-known journal...

...re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting.   ...can be informative, so spend some time thinking about which type of gradient can help answer your particular...overexpression. Before starting a western blot, think about how you’ll interpret the data and what level of ...

...straight from the new search bar, but the menu just above it can help you quickly and easily navigate to educational...down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request...

...cells are avaliable for distribution. To learn more about pooled library screening, read our recent blog post...the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common...

...takes a lot of time and energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags...Pubmed PMID: 23435261. Resources at Addgene Read about other Protein Tags in our Plasmids 101 Series. Get...

...Agreement (MTA) matters: Science thrives on collaboration (it’s the heart and soul of Addgene’s mission...scientists are sharing their materials. Questions about intellectual property rights, pending publications...

...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

...he and his colleagues at Duke University, in collaboration with “cloning guru” Kathleen Ottina and David...it cloning by mail,” Wood says, and it took them about six months from start to finish. They cloned all...

... this study with Addgene. Read on to learn more about their work, and how these plasmids can help cancer...Additional resources on the Addgene blog Learn about our cancer collection pages Read all cancer blog...

...open science Find blog posts about scientific sharing Find blog posts about networking Additional resources...

...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H...

...) [1]. Our initial work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of...PhD student at Harvard Medical School in the laboratories of Stuart Orkin and Daniel Bauer. He received...

...strain. In addition to the collections featured above, many other modular yeast tagging systems have been...Addgene would love to hear from you, our community, about your experience with yeast genome modifications....

...We often hear about breakthroughs in diabetes and lymphoma research or the many different ways you can...little-known disease. You can find more information about the disease on the Meothelioma Cancer Alliance Website...

...Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about our viral...

...intimidating to me, as I had a hard time thinking about how I could fill this time. I was relieved to find...attend to other commitments, without feeling guilty about missing work. As long as my work was finished on...

... will be able to easily find the fruits of your labor and won’t have to repeat the modification. This ...re ever interested in writing your own blog post about a plasmid or set of plasmids you’ve deposited with...

...site-specific recombination in Drosophila, read on to learn about new recombinases suitable for this system. Gerald...recombinases from the Rubin lab For more information about limitations and caveats of Cre usage: Vagaries of...

... over the world. In the course of all those collaborations over the years, he and his colleagues built...Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer that...

...Chapel Hill, where he studies enhancers and blogs about science.     Additional Resources Learn how you...can use FRET to study protein interactions Learn about other types of fusion proteins Study chromatin interactions...

...Additional resources on the Addgene blog Read more about optogenetics New to viral vectors? Browse our Viral...Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources on Addgene.org ...

...Molecular Biology Reference includes information about molecular cloning, how to choose a plasmid vector...straightforward as possible. If you have a question about a specific plasmid element that you would like answered...

...making, and motor control.  What do we understand about Dopamine? DA is produced by specific dopaminergic...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

... posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use...

...These days, it hardly seems like we finish writing about one dual CRISPR-transposon system before another...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science...

...particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular...other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in primer...

...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

...resources on the Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our...on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for...

...Flame awardee Sophien Kamoun of the Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute...Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology Resources on Addgene.org...

...on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based ...Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers...

...the same as not being a bad manager (see cartoon above – what type of bad manager have you had?). You can...Managing scientists I should add a few special words about managing scientists because, let’s face it, we are...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...emission maxima toward the red end of the spectrum (above 600 nm) and increasing the brightness. Arne Fabritius...

...the ABRC are here to support you! From questions about growing conditions, to guiding students through ...local science festivals, ABRC gets the word out about the importance of plant sciences. In 2017 alone,...

...Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

...Our work accelerates and enhances productive collaborations as well as provides visibility for grant-driven...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations such ...