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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 15 results for: Abo

Showing: 1 - 15 of 15 results

  1. Addgene's eBook Collection

    Type
    Guide
    ... | Fourth Edition About Plasmids 101 September 2023 | Fourth Edition About Based on our popular...techniques! Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January...screens. Close About Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent... more! Close About Overlay Select Viral Vectors 101 July 2024 | Second Edition About Viral Vectors...more! Close About Overlay Select Science Career Guide November 2018 | First Edition About Science Career...Close About Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR... trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay ...

  2. Antibody Guide

    Type
    Guide
    ...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...basic protocols and controls follow that of IF (above). Sample preparation, signaling excitation, and ...

  3. Chemogenetics Guide

    Type
    Guide
    .... However, CNO is back-metabolized to clozapine and other clozapine metabolites and these can have off-target...concentrations of clozapine N ‐oxide (CNO). CNO is a metabolite of the antipsychotic clozapine and seems to be...experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating the need for...activity, and do not seem to have the same back-metabolism issues as CNO and are attractive alternatives...chemical variations thereof (FFz, CFz). Learn more about optogenetic systems in our Optogenetics Guide . ...tissue-specific Cre is provided. Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...gamma-retroviral vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors...of plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...increased safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus...packaging methods . The three plasmids described above (envelope, packaging, and transfer) are co-transfected... Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S., Su, L.,...

  5. Molecular Biology Reference

    Type
    Guide
    ...human genome with restriction enzymes would yield about two million DNA fragments, which is far too many...the molecular biologist’s toolbox. To learn more about different types of cloning methods check out our...restriction enzyme digestion. For more information about restriction enzymes check out NEB's website . Insert...endogenous expression levels). For more information about promoters, both bacterial and eukaryotic, as well...can be replicated easily and efficiently in a laboratory setting. Stable - Plasmids are stable long-term...codon. Below you will find helpful resource tables about the genetic code. This table includes the nucleotide...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors...gamma-retroviral packaging systems, and vice versa. For more about gamma-retroviruses, see Addgene's gamma-retroviral...are necessary for lentiviral production in the laboratory, and many have been removed or mutated for increased... genome. To produce lentiviral vectors in the laboratory, the non-required components have been removed...

  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...our adeno-associated viral vector guide to learn about AAV vector components, production and common uses...E2a and VA for replication. For more information about adenovirus, read our Adenoviral Vector Guide . Recombinant... factories for the new viral particles in the laboratory. The cell line used for rAAV vector production...necessary for RABV trans-synaptic transport. Learn more about these and other initiatives in our blog post on ...in the relatively small rAAV capsids. Learn more about optogenetics by watching our Optogenetics 101 video...

  8. Educational Resources

    Type
    Guide
    ...biology, laboratory research, and career development. eBooks Download any of our eBooks to learn about popular...Playlists include: Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular...Technology Career Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction enzymes...

  9. CRISPR Guide

    Type
    Guide
    ...and are discussed in more detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA...Nme2Cas9 (from Neisseria meningitidis ), which are about the same size as SaCas9, with higher fidelity than...targeting range for plant genome engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity...convert one base to another within a target locus of about 8 bp, specified by a gRNA. There are two main types...sequence may not be characterized *In the table above, 3' and 5' indicate on which end of targeted sequence...

  10. Plan Your Experiment

    Type
    Guide
    ...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable...when carrying out CRISPR experiments. Read more about how to design your gRNA . Browse Plasmids: gRNAs...to optimize your delivery conditions. Learn more about CRISPR delivery in mammalian systems . Validate ...

  11. Adenovirus Guide

    Type
    Guide
    ...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...E2a and VA for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral...linearized construct, and rAdV vectors can be harvested about 7–10 days later. Vogelstein designed multiple shuttle...

  12. Science Guides

    Type
    Guide
    ...the following guides to help you start learning about various scientific concepts related to our plasmid...Viral Vectors Find educational guides to learn more about the different types of viruses that are commonly...

  13. Guide to Using Pooled Libraries

    Type
    Guide
    ... to conduct a pooled library screen. The figure above is an outline of how a gRNA library is used. Below...simultaneously in a single reaction. As discussed above, access to this technology is crucial for conducting...

  14. Promoters

    Type
    Guide
    ...of interest is expressed. Summary Promoters are about 100-1000 base pairs long and are adjacent and typically...Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible by tryptophan Promoter ...

  15. Optogenetics Guide

    Type
    Guide
    ... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...
Showing: 1 - 15 of 15 results