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...plasmids for metabolic enzymes and pathways.... Plasmid Collections Synthetic Biology Metabolism Synthetic ...Synthetic Biology: Metabolism SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome...Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains...collection of synthetic biology plasmids related to metabolic pathways and components. Examples Include: Biofuels...Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword or sort ...

...Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read about the Advocating for Science... of $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This was due to the threshold...postdocs - and in some cases, faculty - have found out about what their institution is doing from Future of Research...Science Symposium and Workshop Learn about a Variety of Science Careers Resources on Addgene.org Find Career...

... other are collaborating on projects. When I was a graduate student, I led a collaborative project between...funding and circumstances permit, think about having collaborators spend time with each other in person....meet up with collaborators. Being able to present data from multiple aspects of a collaboration and immediately...the Addgene blog Read our blog post about external collaborations Browse our science career related blog...communication a non-negotiable part of your collaboration. Luckily, there are techniques and tools that...same page. Ideally, these meetings include all collaborators, from the lab members who are conducting the...interesting results or ask questions to your collaborators. This helps everyone feel included in the project...

...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly... and collaboration management. For example, you may already be part of a scientific collaboration - show...friendly, and helpful. Finally, don’t forget about collaborations and projects you can do with others after...lead! Read our blog post about fostering communication in remote collaborations! Stay in touch: Maintaining...notes that remind me about the current state of a partnership. Every collaboration and partnership is different...favored by research-intensive careers. External collaborations are becoming increasingly important as more...scientific project to the next level. External collaborations and partnerships are also very important in...

... she spoke with Teytelman about an opportunity to work with a collaborative group, she also joined early...guide on Transferable Skills: External Collaborations Learn about the 2018 Minisymposium on Scientific ...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...individuals with similar ideas to actually do something about it. Building a diverse team with different talents...the eLife ambassadors group was already thinking about ways to tackle irreproducibility. Benjamin Schwessinger...grassroots efforts. After thoughtful conversations about reproducibility with Teytelman, Schwessinger joined...previous work at Center for Open Science and Sense about Science. However, she realized that she couldn’t...

...else to focus on. Use collaborative cloud apps It is a good idea to use collaborative cloud tools such as...creative collaboration in the workplace. There are many other ways to encourage collaborations remotely...meeting? No problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira. ...serve as a springboard for future discussion. Collaboration and creativity are pivotal in writing publications...Vicens et al., 2007) geared towards enhancing collaborative writing and this recent article published in...approaches/tools we have to foster creative collaboration remotely in science are endless. Let’s adapt...blog post “Fostering Communication in Remote Collaborations” to get more ideas. Thank you to our guest...

...use Twitter professionally to network, learn more about alternative careers for scientists, and share cool....” This paper addresses the following questions about scientists on Twitter: who are they? What do they...

...the Addgene Blog How did this change in labor laws come about? In March 2014, the White House issued a...must be above this level, and certain job duty tests must be met. In the Department of Labor’s general... to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect...ensuring that either all affected scientists are paid above this threshold or for tracking the hours that these... a memorandum to the Secretary of Labor to update the FLSA, which had not seen revisions since 2004. In... In July 2015, the Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by ...adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations of...

...successes in metabolic engineering have been limited to the overexpression of various metabolites in Escherichia...species or metabolic pathways. A new study takes us one step closer to the vision of metabolic biofactories...cellular behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors...be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and E. coli...control via scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated...demonstrated the potential of this to control a metabolic network in S. cerevisiae by using three RNA programs ...programs, the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives...

...our blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...needed. In the example above, for example, we fostered a formal scientific collaboration between two research...project. We think that these collaborations add enormous value. A discussion about a protocol can save weeks..., the researchers did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform...approach was useful in humans. Then, one of them heard about our online platform, called Crowdfight, and filed...just the right infrastructure and offered to collaborate with them. The partnership was fruitful and a...This example clearly illustrates the power of collaboration, which despite the competitiveness of cutting-edge...

... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

...Connie Cepko to learn about her mentoring style. Top 7 questions scientists ask me about mentoring In this... answers to the top questions scientists ask me about mentoring. Here they are: What makes a good Mentor... Where can I find a Mentor? What is this I hear about “group” or “peer” mentoring? How do I ask someone...a good science mentor? I started a conversation about many of these topics with the researchers who joined...often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela Depace's...questions in future posts. If you have a question about mentoring that you want us to cover, let us know...

...new conference rooms and learned some fun facts about these genes and the origins of their names!  ...

...), Antibodies: a Laboratory Manual, 2nd Edition. 2104. Cold Spring Harbor Laboratory Publications, New...production and subsequent purification.  Academic laboratories and companies have already begun creating plasmids...Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: What is an ...

...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...basic protocols and controls follow that of IF (above). Sample preparation, signaling excitation, and ...

...exon coding for an essential protein domain could abolish protein activity and essentially function as a ...reference sequence you used for gRNA design. Read more about how to design your gRNA . Synthesize and Clone Desired... other downstream methods. For more information about viral vectors and their production, see our viral...and Proteins In addition to the methods described above, you can also directly deliver mature mRNA or purified... approach. Similar to the mRNA method described above, this method is also transient, as the RNPs will...Browse Cas9 plasmids for RNP delivery . As mentioned above, CRISPR efficiency will vary based on the method...information, take a look at our collection of blog posts about CRISPR expression systems and delivery methods ....

...Addgene's collection of KLF plasmids and learn more about the scientists who have created and shared these...Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are a family of ...proliferation, differentiation, inflammation, metabolism, fibrosis, atherosclerosis, and carcinogenesis...transcriptional activation and/or repression. Continuing collaborative efforts within the KLF community have lead to...to contribute to this community resource. Learn about the benefits of depositing plasmids with Addgene...

...integrating MDH-related research into their teaching laboratories. They encourage instructors teaching different...research community and facilitates scientific collaborations between institutions. With these plasmids, ...regulation and post-translational modifications, and metabolic pathways and protein interactions. Plasmids A ...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and more....2021.43 (Link opens in a new window) External Collaboration Results in Student Learning Gains and Positive...

...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...Learn more about synthetic biology resources available at Addgene. Browse featured collections or depositing...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...Schmidt-Dannert Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening Christopher Voigt Clifford Wang...

...sensors for live-cell imaging Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP ...protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion profiling. ...David Savage Maltose Monitor maltose levels and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer...Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism in Plants. Plant Cell. 2020 Aug 13. pii: tpc.20.00241...concentration Semisynthetic sensor proteins enable metabolic assays at the point of care. Science. 2018 Sep...biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes. Nat Commun. 2024 Dec 19;15...biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion profiling. ...