We narrowed to 731 results for: Sis;

1. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   ...: Create promoters with the help of epigenetic analysis and designed using stretches of DNA that were ...

2. Engaging with science and society at pgEd

   Type

   Blog Post

   ...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...

3. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   ...PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ...

4. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   ...positive selection marker such as an antibiotic resistance gene is also included to select for successfully...

5. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   ...contribute to our understanding of diseases and even assist in drug discovery. References 1. Baird, Geoffrey...

6. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   ...access to rich and complex microbiome data for analysis. And, well, cats... everyone knows the internet...

7. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

   Type

   Blog Post

   ...literature review, survived technical feasibility analysis, and was a marketable product. It was a small ...

8. Common Injection Routes in Mice

   Type

   Blog Post

   ... & Lopez-Berestein, G. (2020). A retrospective analysis for different routes of administration in Mice-Percutaneous...

9. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   ...inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus...

10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    ...a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence...

11. Pooled Library Amplifications

    Type

    Blog Post

    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

12. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    ...available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue...

13. Of Myc and Men

    Type

    Blog Post

    ...explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding...

14. PhD Applications After COVID

    Type

    Blog Post

    ...in-person events change seemingly daily (and are inconsistent between universities!) This guide tells you ...

15. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    ... happening even though I met with X on a weekly basis! The silence and maybe even the slight awkwardness...

16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    ...., 2000). Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic...

17. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    .... An imperfect solution is to restrict imaging analysis to cells expressing the lowest levels of the fusion...

18. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    ...is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are listed, as...

19. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    ...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...

20. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ...Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University of Miami Miller School...

21. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...describing the development of “transcriptors,” transistor-like digital genetic switches that enable cellular...

22. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    ...At its core, harvesting recombinant antibodies consists of separating antibodies from media, cells, and...

23. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    ...course recently used a combination of phylogenetic analysis, structural prediction, and bench work to identify...

24. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    ...Organization Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning Resources...

25. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    ...five Addgene colleagues who read it with me, the consistent response from scientist and non-scientist alike...

26. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months...

27. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    ...to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting...

28. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    ...elephants than it is to design one demonstrating mitosis.   Consider also the amount and type of information...

29. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    ...which of these 13,000 regions are critical? One hypothesis: the enhancers! This leads us to a three-step...

30. Plasmids 101: Colony PCR

    Type

    Blog Post

    .... If you are going to complete your colony PCR analysis in the same day, you can save the leftover bacteria-water...

31. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ...made all the difference here at Addgene is the emphasis placed on team building throughout the years. ...

32. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    ...deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently...

33. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    ...Our viral vectors data is less amenable to such analysis, due to its inherent bias: Addgene uses a prioritization...

34. Hot Plasmids Spring 2024

    Type

    Blog Post

    ... research; however, microglia are notoriously resistant to viral transduction. Through in vivo screening...

35. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    ...department] at [name of the university].During my thesis work on [phenomenon X], I read several of your ...

36. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ...destination vector carry different antibiotic resistance markers (indicated here by plasmid color), allowing...

37. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    ...Washington) but as discussed elsewhere, there seems no basis for this decision in FLSA, and at the time of writing...

38. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    ...fast turnover of projects, pressure to maintain consistently high levels of performance, and occasionally...

39. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    ... Consider, for instance, a disease like cystic fibrosis, which can be caused by thousands of different...

40. Lab to Office Culture Shock

    Type

    Blog Post

    ... I find that my vocabulary improves on a weekly basis. There will be meetings. And some more meetings...