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We narrowed to 1,023 results for: CAT;

Showing: 801 - 850 of 1023 results

  1. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...published, knockout validated, and work in your application. But…they are the same isotype and therefore ...are identical for antibodies within certain classification groups called isotypes.  Why does isotype matter...

  2. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...launching a company without partners.  You need a dedicated team that you know well and trust.  This will ...Chowdhury and Clinton Buell, who have very diverse educational backgrounds (an essential component to any successful...

  4. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...will you meet? How does the Mentor prefer to communicate between meetings (email, phone, etc.)? Make agendas...skills Support inclusion, diversity, and open communication Feel valued by giving back Final tips for success...

  7. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator Non-science favorites...times."     - Aliyah Weinstein, Marketing and Communications Manager Addgene's book club Indeed, reading...

  9. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...generation when bound by the dL5** FAP.  The iodine-modifications also shift MG-2I’s excitation wavelength to ...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-2I-dL5...

  11. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...of Toronto. He is an author of 20+ scientific publications and patents.   ...

  12. The MTA at Addgene

    Type
    Blog Post
    ...things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar agreements...portal automates a lot of the process, from notifications and emails to generating the UBMTA link for ...

  13. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...achieving the gRNA representation recommended in the publications associated with these libraries. Please note...

  14. How to Lead a Great Meeting

    Type
    Blog Post
    ... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ...

  16. Antibodies 101: Isotypes

    Type
    Blog Post
    ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of these five isotypes, such as the number and location of disulfide bonds, the number of constant domains...

  18. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages...

  19. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...

  20. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ...

  21. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...insert. All colonies on this plate should be blue, indicating that your IPTG and x-gal are working as they ...of false positives: Blue-white screening only indicates the presence of AN insert, not necessarily YOUR...

  24. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...might even be expected to write up a report to communicate your findings to your colleagues. So perhaps ...about the issue, assessed potential protocol modifications, and ultimately decided on the changes to implement...

  25. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...AsCas12f1. They found that this system, without any modifications, is effective in genome editing in both bacteria...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

  26. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are...

  28. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...radioactivity in the culture supernatant (this is indicative of protein degradation). To control for protein...studying autophagy. This method relies on the identification of autophagic structures based on morphology...

  29. All in a Twist: dsRNA

    Type
    Blog Post
    ...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...

  30. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...for a scaleable lab. When you need to track the location, quality, growth, and legal status of thousands...stab culture Ship the stab culture to the proper location To keep ourselves from mixing up samples and ...

  31. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    .... To remedy this, Shah et al. provide a table indicating the sex of the most commonly used cell lines ... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities...

  33. Working with Nuclear Receptors

    Type
    Blog Post
    ...murine members. NRs permit the integration and communication of such signals between central and peripheral...macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa ...

  34. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...accomplishments and demonstrate your science communication skills. Know your audience. Connect your prior...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...

  36. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...the most common type of competitive ELISA is a modification of the sandwich ELISA as described here, the...ELISA, the only difference being the additional amplification step, which helps to visualize low-abundance...

  39. Lab Automation at Addgene

    Type
    Blog Post
    ...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...

  40. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...assemblies are possible, the two sets of data together indicate that 35-pieces is a reasonable upper limit for...Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https...

  41. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...combined with. Combining a PSAM with the IPD of a cation channel, such as the serotonin 3 receptor, will...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

  42. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...type are generally broken down into two broad categories, prokaryotic or eukaryotic, based on the functional...termination mechanisms fall under two general categories: rho-dependent and rho-independent. Rho factor...

  43. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...particle. Consequently, titers based off of p24 quantification tend to be overestimates since they can include...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...

  45. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...Anything between orange and light orange usually indicates that the cells have grown enough, are making their...

  46. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...

  47. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...brightness with increasing depth caused by light scattering and absorption.  Finally, the photomultipliers...

  48. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...search to check for expected ORFs, mutations or truncations. A primary advantage of blastx is that you do...other labs speed up their plasmid and cloning verification steps and free up more time for using your plasmids...

  49. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...

  50. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and... Pseudotypes: Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene...
Showing: 801 - 850 of 1023 results