We narrowed to 1,024 results for: PLE;

...cultural shifts that can have bigger impacts. For example, if you joined an office where everyone used a ... about this, and even has a list of biological samples that are stored long term at -70 °C. My Green Lab...

...contributor of careers blog articles, Joanne had plenty of advice for the group of juniors and seniors,...working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their...

... in the wired world we are living in, there are plenty of other ways to define and measure scientific ...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...

...experiments. Finally, we were thrilled by how many people accessed our freely available online collections...the promise of science to help a great number of people. I look forward to working with Addgene colleagues...

...allow the extraction of enough plasmid to last for multiple experiments. Another fundamental aspect for transfection...nucleic acid and chemical contaminants, these DNA samples must also be entirely endotoxin free. Endotoxin...

... While those are the biggest changes, there are plenty of less obvious ones to discover as you browse.... Multiple Authors...

... which display sophisticated interactions with multiple other proteins. Again, little to no change in ... place to start. These tags are easy to use in multiple common applications. Due to their limited effects...

...metagenomic datasets to try to determine whether simpler and maybe smaller Cas systems could exist in nature... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But...

... so on, differently than scientists would. For example, “manipulate” could mean “illicit tampering” to...founder of The Addictive Brain. Prior to this, he completed PhD from Philipps-University Marburg, Germany ...

... never advertised. You have to meet interesting people and maintain diverse relationships to have any ...opportunities. Don't wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens...

... to mind! Attendees consistently mentioned how pleased they were to learn about these exciting scientific...science, all you need is a great idea and a group of people willing to make that idea a reality! How do you...

...heart and soul of Addgene’s mission), but legal complexities can arise when scientists are sharing their ...facilitating the exchange of not just the physical samples, but also the legal documentation that allows you...

...addition of histone modifiers. “We want to allow people to prove the causal role of specific epigenetic...combine with different effectors. I’d encourage people to get creative with them for their own uses.” ...

...after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog...and Lukas Morgan There are many model organisms people are using for their research. In this blog post...

...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...ability to survive in certain environments, for example. “We’ve been contacted by investigators all over...

...challenging – is advice and guidance from only 2 people enough?  For many years I’ve been organizing mentoring...who did not serve as good mentors, but many had examples of very productive mentoring relationships with...

...arms that flank the donor plasmid cargo are complementary to either side of the double-stranded break ...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2...

...are appying for diffrent types of positions (for example, bench research vs. project managment) your resume...a specific department and/or location. The more people you meet the better chance you have of finding ...

...microbiology, virology, and genetics - before going on to complete her Masters degree at Imperial College London ...ideas with others. This love of interacting with people and talking about science makes her the perfect...

...article is published. This policy gives us time to complete necessary quality control and legal approvals ... your trust, and meet the community’s needs, so please let us know what you think. We appreciate your ...

...food pantry or community arts organization. Many people are surprised to learn that Addgene is officially...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...

...promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein... However, this system is far from perfect. For example, If your fusion protein is expressed at low levels...

...this frequency can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though...neurotransmitters in various species and while exhibiting complex behaviors. Find dopamine and other biosensors in...

... publications cited. If the information seems incomplete or unclear, Bairoch follow up via email to the... Multiple Authors...

...organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag system, showing...Resources page. Here are some highlights from the past couple months: A new lentiviral CRISPR library from Alec...

...serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments. Widening...successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular biology and metabolism...

... are leaders in the field of microbiology with multiple high-profile publications. My classmates consisted...portion of the paper will be generated. After you complete a publication quality figure, produce the figure...

...from some Addgene support? Please get in touch, as we aim to support a couple of events a year for the ...

...be successful in a specific role or cycle. For example, if you try to lead when you are expected to be...Got You Here Won't Get You There: How Successful People Become Even More Successful, by Marshall Goldsmith...

...has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection...e.g. pLKO.1 - TRC cloning vector). Check out our complete guide to empty backbones for additional details...

...library is the MORF library, which stands for Multiplexed Overexpression of Regulatory Factors. The Feng...useful for screening for binding properties. For example, several coronavirus libraries have been used to...

...fluorophores that excite in the violet range. For example, mWasabi can be used with Sapphire (excitation ...would like to share with the scientific community, please deposit the plasmids at Addgene! References Ai...

...(on a schedule) I hope you all know by now that people get jobs at least 85% of the time because of relationships...pretty easy to make 30 minute appointments with people all over the world. Think about contacting alumni...

...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... Department of Science Communication where he completed a master's degree in 2020, his research explores...

...ever like to write about a recent plasmid deposit please sign up here.  Human whole genome sgRNA iBAR ...allowed tracing of the performance of each gRNA multiple times within the same experiment by counting both...

...expertise you’d like to contribute to the blog, please email us and we’ll get you writing!     www.addgene.org... Service or any of the materials we distribute, please send an email to [email protected]. Your questions...

...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...PCR product. If you need to decrease error rate, please use one of these methods to ensure your insert ...

...efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is...

... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...representation is most easily understood visually. As an example, the curves from our recent amplification of the...

...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

...change to a slide, think about whether the same principle applies to other slides in your deck.  If someone...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

...inspires students and teachers to apply engineering principles to biological systems. Today, what started in... in March to exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub...

...Also, if there is a chance you need to combine multiple transgenes in one fly, having the transgenes in...designing experiments where you want to express multiple transgenes, remember that you’ll need to integrate...

...described xCas9, which displayed the ability to target multiple PAM sequences (NG, GAA, and GT), greatly increasing...better suit specific experimental approaches. One example is CasX, characterized by the Oakes and Doudna ...

...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

...phage mutation rate Phage infection results in a complete ABE protein. If the phage delivers an active variant...ABE8e. ABE8e is not only fast, it can also be multiplexed: when tasked to correct two point mutations that...

...and transgene expression (Zhou et al. 2017). For example, deleting the B-B' or C-C' arms can reduce the ...AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019), and different...

...of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene...double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control...