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We narrowed to 1,025 results for: URE

Showing: 801 - 850 of 1025 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...than the other (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv...of panning and scFv characterization.    Figure 2: Phage display starts with a pooled library of...Bates A, Power CA (2019) David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody ...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often leads...database, which relies on CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping...examined the ability to use sequence-based and other features to nominate gRNAs that are likely to be active...

  4. CRISPR Guide Update

    Type
    Blog Post
    ...accompanied by new or refreshed figures made using BioRender.com. Figure 1: PRIME editing Please...

  5. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian use. On the second...Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR...

  6. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ... significantly away from the functionality and features that previously made the platform a mainstay of...not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated with...

  7. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...system for locating and containing escaped flies. Nature 4:1-22. https://doi.org/4.01.2022/hafd...

  8. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...sharing all the incredible guest posts and stories featured in our blog in 2023. Some of these were written...Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes...

  10. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ... by Xi Chen (@seleena78)   Honorable Mention: Culture Warrior The Moore lab, who was full of holiday ...honorable mention for their second submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire...

  11. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ..., you will be able to search for your favorite features, such as serotype, promoter, or tool type. Drilling...accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, we hope to...

  12. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...guide page. For more protocols and information, be sure to check out the Trono Lab website. If you have ... plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours...

  13. Deck the Lab 2022!

    Type
    Blog Post
    ...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...@seleena78)   Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram...

  14. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...Fun stats and trivia begins! Tuesday: Depositor features (plasmid deposits and blog posts showcasing the...year in recent years. Despite COVID-19 and lab closures, we’re expecting to reach ~14,000 plasmids deposited...

  15. Our New Antibody Mascot is....

    Type
    Blog Post
    ...  Fig. 1: Meet...well, frankly, we're not sure yet!   This as-yet–unnamed friend is a huge ...mascot, an antibody joining us on our scientific adventures.  You can submit your name suggestions here or...

  17. Editor's Choice, October 2016

    Type
    Blog Post
    ...party (see all of our wonderful costumes in the pictures below, yours truly is dressed as Unikitty from...Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, ...

  18. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe...Christmas tree! It is that time of the year again: miniature #Christmas tree goes up at home!This year with...

  19. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ... 26:10 : What's been your biggest experimental failure? 26:11 - 31: 32 : What's been your biggest experimental... Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the Research Process...

  20. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ... are some good guidelines to follow in order to ensure that people can quickly and easily identify what...reading the name. For more information about plasmid features and tips you can use in the lab, check out more...

  21. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...compared to monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids... from this Addgene blog post Read the paper in Nature Methods Find the anti-CRISPR plasmids Tagging...

  22. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...here: https://www.youtube.com/watch?v=lQGAsgjjBDI&feature=youtu.be. 8:17 - 11:10 : The 3rd year of graduate... Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the Research Process...

  24. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...Seeing a real lab!” And several of our visitors made sure to tell us what we already knew about our Shipping... guests, as did how much work is put into making sure scientists all over the world know that we can help...

  25. A Scientist's Guide to Ebola

    Type
    Blog Post
    ..., along with links to the relevant scientific literature.  -In light of the current Ebola crisis, Science... anyone interested in EBOV’s past, present, and future, don’t miss Carl Zimmer’s coverage on the subject...

  26. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...present, but the real promise of Addgene lies in our future. As we near our 20th anniversary, we have many ...wealth of history to learn from, and a compelling future to plan in the changing landscape of science. We...

  27. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...experiments; large amounts of virus are required to ensure that you get full library coverage in pooled library...and genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903...

  29. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ... RNA binding domains depends on the secondary structure of RNA. As a result, targeting the RNA of interest...better understanding of the phenomenon soon.  Rest assured, as the PUF protein design tools continue to evolve...

  30. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...started making regulations such as preventing manufacturers from dumping hazardous waste directly into the...levels. Disposables are an accepted part of lab culture, but with the right incentives (for example, by...

  31. Reflections and Looking Ahead

    Type
    Blog Post
    ...sharing research materials — we're accelerating the future of science. Want to learn more about our latest...consensus platform for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095...

  33. Editor's Choice, September 2016

    Type
    Blog Post
    ...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights... remember the hazards of that chemical in your obscure growth medium, these apps can help make your lab...

  35. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...It could [be used to screen for] morphological features, migration, or survival in different contexts....

  36. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ...focus wholly on the science I love. This immersive nature of academia often means that scientists-in-training... video call. If you do decide to use Skype, make sure to follow the rules of successful, remote conversations...

  37. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...agencies with over $100 million in annual R&D expenditures to support increased public access to the results... guided by aspirations to promote open science, ensure good stewardship of clinical trial data, serve ...

  39. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...testing multiple gRNAs targeted to the same locus to ensure their effects are specific to that locus. The data...spreadsheet for submitting your gRNA sequences here. Features of the validated gRNA table You can access the...

  40. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ... can’t find the molecular cloning guide you were sure was in one of those four white binders. Thirty minutes...) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on the spine. ...

  44. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...need to be able to replicate in Agrobacterium to ensure that they are maintained during co-cultivation ...plasmid can also be customized due to its modular structure. Two multiple cloning sites (MCS's) flank the ...

  46. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ... a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman MD (2020) Adeno-Associated Virus Genome...

  48. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...Publication policy with two changes that will help us ensure that plasmids don’t get left behind in this status...Addgene! We'll continue to review our policies to make sure they meet our goals, maintain your trust, and meet...

  49. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...with the small solubility tag GB1. In SunTag nomenclature throughout the rest of this blog post, GFP refers...the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4...
Showing: 801 - 850 of 1025 results