Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 16 results for: URE

Showing: 1 - 16 of 16 results

  1. CRISPR Guide

    Type
    Guide
    ...addition of a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased ...synthesized (Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries...Berkeley. Plan Your CRISPR Experiment Resources Figures All figures were created using BioRender.com (Link opens..., N., Ishitani, R., Zhang, F., & Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA...vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. PMID: 25830891 Rauch...became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR mechanism Engineered...gRNA left free to interact with the target DNA. Figure 2: Overview of the NHEJ repair mechanism Cas9 will...

  2. Antibody Guide

    Type
    Guide
    ...isolated and placed in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is...Antibodies can also be produced in cell cultures using hybridomas, cell cultures created by fusing B cells with...form a “Y” shape, as shown in Figure 1. The two arms of the Y structure are responsible for binding the...Monoclonal antibodies are generated from cultured B cells, where each culture should produce the same antibody...Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared... a signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary ...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...Table 1. Figure 4: Third-generation lentiviral plasmids. Created with BioRender.com. Feature Second-generation... chromatin architecture through large-scale mapping of lentiviral integration sites. Nature Protocols ...to continue their lifecycle and replicate. This feature can be harnessed for stable delivery of various... typically ranges from 8–10 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...have been removed or mutated for increased safety. Figure 1: Wild-type lentivirus genome. Created with BioRender.com...largely intact. First-generation plasmids include (Figure 2): Transfer plasmid — contains transgene and wild-type...envelope removed Envelope plasmid — contains env Figure 2: First-generation lentiviral plasmids. Created...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...replication and also act as signals for packaging. Figure 1: Wild-type AAV genome. Created with BioRender.com...do not trigger a strong immune response. These features have made AAVs the preferred choice to be used...components, see the AAV Plasmid Elements table . Figure 2: Plasmids required for rAAV vector production...methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid is compatible with...available in your lab. When cloning your plasmids, be sure to use recombination deficient bacterial strains... the ITRs found in transfer plasmids. This will ensure that the repeats will be maintained and often results...and instead provided by the chosen cell line to ensure safety and prevent the potential formation of replication-competent...

  5. Adenovirus Guide

    Type
    Guide
    ...Collection page . Figure 5: Overview of AdEasy™ adenoviral vector production system. Figure is based on the...assemble icosahedral capsids and build new virions. Figure 1: Wild-type adenovirus and its genome. Genes over...which incorporate additional genomic deletions. Figure 2: First-generation rAdV vectors. Created with ...using first- and third-generation rAdV vectors. Figure 3: Second-generation rAdV vectors. Created with... challenge of the HCAdV vector production is to ensure no carryover from the HV in final viral preparations... genome or using other plasmids instead of HVs. Figure 4: Third-generation rAdV vectors and helper virus...methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid is compatible with...

  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...to continue their lifecycle and replicate. This feature can be harnessed for stable delivery of various...gamma-retroviruses ranges from 9–11 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus genome. Created with...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA...infectivity for different cell types (known as tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com...methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid is compatible with...available in your lab. When cloning your plasmids, be sure to use recombination-deficient bacteria strains,...

  7. Optogenetics Guide

    Type
    Guide
    ...Guide to Channelrhodopsin Variants: Features, Limitations and Future Developments. Exp Physiol. 96(1): ...optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons) and their...optogenetics research utilize light to detect, measure, and control molecular signals and cells in order...functions. Example schematic of an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed... allow the fast depolarization of neurons upon exposure to light through direct stimulation of ion channels... in excitation (depolarization) of the neuron. Feature enhancements include: Increased photocurrent amplitude...from the cryptophyte Guillardia theta ). Other feature enhancements include: Increased photocurrent amplitude...

  8. Plan Your Experiment

    Type
    Guide
    ...interest is PCR amplified, PCR products are denatured-renatured, treated with a nuclease that cleaves DNA...reagents are appropriate for a given experiment. Make sure to check whether reagents are available to carry... gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then delivered to...match the target locus, but it is also critical to ensure that the gRNA target sequence does NOT match additional...allele may be different owing to the error-prone nature of NHEJ. In HDR gene editing experiments, most ...protein-based genome editing in vitro and in vivo. Nature Biotechnology . 33 (1), 73–80. PMID: 25357182...

  9. Chemogenetics Guide

    Type
    Guide
    ...check out our Chemogenetics Plasmid Collection . Figure 1: General overview of a chemogenetics experiment...muscarinic receptor and has not been used in vivo . Figure 2: DREADDs, their ligands, and signaling properties...and/or K + ions, and activates neuronal activity. Figure 3: PSAM4-based ligand-gated ion channels, their...optogenetic systems in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic... control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or... X, Stone O, Roth BL, Jin J (2015). The First Structure–Activity Relationship Studies for Designer Receptors...

  10. Molecular Biology Reference

    Type
    Guide
    ...propagation of these plasmids through cell division. To ensure the retention of plasmid DNA in bacterial populations.... Under these conditions, there is a selective pressure to retain the plasmid DNA, despite the added replication...organisms, including plants, worms, mice, and even cultured human cells. Although plasmids were originally...bacteria, but also provide those bacteria with a pressure to maintain and replicate your plasmid over multiple... extremely important when verifying plasmids to ensure each plasmid contains the essential elements to...replicated using the modified bases and a microscope captures the fluorescent color that is emitted each time...

  11. Science Guides

    Type
    Guide
    ...conjugated to a signaling molecule, can be used to capture, quantify, and identify proteins of interest. Read...Optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons) and their...optogenetics research utilize light to detect, measure, and control molecular signals and cells in order...

  12. Addgene's eBook Collection

    Type
    Guide
    ...When you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!...Addgene's eBook Collection When you're not sure where to start or need a handy reference, Addgene's eBooks...you need to know about plasmids, from parts, nomenclature, and uses to common promoters and cloning techniques...

  13. Guide to Using Pooled Libraries

    Type
    Guide
    ...infected cell receives only one plasmid. To make sure that every plasmid is adequately represented in ...planning to conduct a pooled library screen. The figure above is an outline of how a gRNA library is used...cells that survive post-selection. The selective pressure must be strong enough that most of the cells die...

  14. Promoters

    Type
    Guide
    ...the TSS. Eukaryotic promoters are so complex in structure that the DNA tends to fold back on itself which...which is a main component of a cell’s ribosome structure. Ribosomes are the site of protein syntehsis where...

  16. Cloning

    Type
    Guide
    ...protocol on our website. Ligation Independent Cloning (Figure adapted from LIC protocol ) Back to Top Yeast-mediated...
Showing: 1 - 16 of 16 results