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We narrowed to 1,092 results for: addgene

Showing: 801 - 850 of 1092 results

  1. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion of software... Clement Andy May Additional resources on the Addgene blog Read our CRISPR 101 blog posts Learn about...gRNAs Find other CRISPR software Resources on Addgene.org Find CRISPR plasmids and resources Download ...

  3. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter...Internet "trolls"   More resources on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro...

  4. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...Elsevier, pp 215–247 Additional resources on the Addgene blog Droplet Digital PCR for AAV Quantification...Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...advancement. Andrew Hempstead is a Senior Scientist at Addgene.  Andrew's interests include genome engineering...PMCID: PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about...techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs...

  6. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references 1, 2....PMCID: PMC5609467. Additional Resources on the Addgene Blog Using virus in your research - a primer for...troubleshooting viral transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...this paper are available at Addgene.  Additional Resources on the Addgene Blog Learn about Engineered...about Homology Directed Repair Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids...

  9. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ...Collection Get Tips for Using FRET Resources on Addgene.org Browse the Allen Institute for Cell Science ...

  10. Of Myc and Men

    Type
    Blog Post
    ...Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the...scisignal.aax9730  Additional resources on the Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...choosing a lab Resources on Addgene.org Find open positions at Addgene Check out our career videos ...neurodegenerative diseases.   Additional resources on the Addgene blog Check out all careers related blog posts ...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...PMCID: PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez ...: A Practical Guide Additional Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Validated...

  13. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

  14. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which...Overview of Colony PCR Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC ...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique...

  15. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

  16. Genetic Code Expansion

    Type
    Collection
    ...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression...s41392-024-01780-w Additional resources on the Addgene Blog Overcoming the AAV Size Limitation for CRISPR...Using the FITS Approach Additional resources at addgene.org CRISPR pooled libraries Guide to pooled libraries...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    ...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...s41586-024-07259-6 Additional Resources on the Addgene blog Prime Editing: Adding Precision and Flexibility... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...

  19. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...luck and happy binding! More resources on the Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting...

  21. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...Nicola Patron, who shared the MoClo plant kit via Addgene. For us, transient leaf transformations are a great...PMCID: PMC2699436. Additional resources on the Addgene blog Read all of our plant biology blog posts ...Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources page...

  22. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...7th grade biology teacher. I led up to my job at Addgene, which is likely a career path none of them had...guidance and feedback. Additional resources on the Addgene blog Browse our CRISPR blog posts Download our...science communication blog posts Resources on Addgene.org Check out our comprehensive CRISPR guide Find...

  23. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...outdoor photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check...

  25. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...s13059-020-01973-w Additional Resources on the Addgene Blog First time CRISPR user? Check out our tips... Here Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page ... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...

  26. Hot Plasmids - November 2023

    Type
    Blog Post
    ...43R] has been one of the hottest antibodies in Addgene’s collection since we began distribution and has...[K28/43R] under the Application section of the Addgene antibody page, and learn more about the whole-brain...

  27. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-...Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance Plasmids...

  28. How to Be an Excellent Trainee

    Type
    Blog Post
    ...biology you will study. (You can also check out Addgene’s blog, eBooks, and other educational resources ...personnel! Happy training! Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition...

  29. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral...Resources and References More resources on the Addgene blog Viral Vectors 101: Viral Vector Elements ... This blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...

  30. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...Central PMCID: 3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs ...AAV, and much more! Find ready-to-use AAV from Addgene here or check out these protocols if you want to...Viral Vector Blog Posts Additional Resources on Addgene.org Search for AAV Vectors to use with your research...

  31. DNA Purification Without a Kit

    Type
    Blog Post
    ...DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as a column-based plasmid...in the comments! Additional resources on the Addgene blog Read our Plasmids 101 blog series Find other...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning...

  32. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...strategies for your experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock....3390/ijms21186461 Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page ... CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...

  33. MXS Chaining

    Type
    Blog Post
    ...Resources on Addgene.org Browse our Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid...and BamHI EcoRI Additional Resources on the Addgene Blog Read about Gibson Assembly Learn About Other... you use MXS Chaining by emailing us at blog@addgene.org. Table 3: Chaining Based Cloning Methods ...

  34. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Find out More About Cancer and Addgene's Cancer Collection Pages... Screening in Ghana Additional Resources on Addgene.org Check Out Our Cancer Ressearch Collection Find...

  35. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...become a positive force for public health. Visit Addgene's Microbiology Pages What is antibiotic resistance...Resistance Fact Sheet  Additional Resources on the Addgene Blog Learn How Antibiotic Resistance Genes Are...Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ...

  37. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing your own CRISPR... the Dish blog biobabel blog Resources on the Addgene Blog Catch Up on Your CRISPR Background with CRISPR...Methods Validate Your Genome Edit Resources on Addgene.org Find CRISPR Plasmids for Your Research Check...

  38. The Challenges of Cell Culture

    Type
    Blog Post
    ...pharmacology (many of which are represented in Addgene's Special Collections). Arguably, oncology has benefited...Abcam Cell Culture Guidelines Resources on the Addgene Blog Learn All about Mammalian Vectors Read up...Using GFP for Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

  39. CRISPR Antimicrobials

    Type
    Blog Post
    ...PMC4˚237163. Find plasmids from this paper at Addgene. 2. Bikard, David, et al. “Exploiting CRISPR-Cas...PubMed: PMID 26973885. Additional Resources on the Addgene Blog CRISPR Methods for Bacterial Genome Engineering... out of the Genome  Additional Resources on Addgene.org Find CRISPR Plasmids for Your Research Read ...

  42. Plasmids 101: Protein tags

    Type
    Blog Post
    ...using plasmids found in this paper (available at Addgene).   Table 2: Protease recognition sites commonly...purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts of interest: Which Fluorescent...

  45. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...101: Validation Resources on addgene.org Addgene's Antibody Guide Addgene's Antibody Protocols...10.1038/s41598-019-55133-7 More resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

  47. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...top-performing constructs are now available through Addgene (pKb-TnpB1 and pKb-TnpB2). How TnpB Works: The...025-02023-8    Additional Resources on the Addgene Blog CRISPR 101: Engineering the Plant Genome ... in Popular Culture Additional Resources on addgene.org Genome Engineering Guide Plant Plasmids and ...

  49. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...  Find broad host range recombinant vectors at Addgene!   References and resources References Doran, ...10.1007/s13205-023-03507-0  More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids...

  50. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... Resources on Addgene.org Download the Plasmids 101 eBook  Browse plasmids at Addgene Read our Molecular...Kamruzzaman et al., 2017). Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Learn...
Showing: 801 - 850 of 1092 results