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We narrowed to 843 results for: ANG

Showing: 321 - 340 of 843 results

  1. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...you to better analyze your results; it does NOT change anything in your samples. This means that, unlike...Figure 1B). For single-color samples, you can only change the compensation values for whichever fluorophore...can click on the correct box and either manually change the compensation values or adjust the values with... help you get started. Remember, you can always change your compensation values during the analysis portion...

  2. PhD Applications After COVID

    Type
    Blog Post
    ...standards for hybrid, virtual, and in-person events change seemingly daily (and are inconsistent between universities...both sets of interviews. I also had universities change their format of interview from a travel day to ...response to challenges on their end. Though the quick changes in plans was stressful in and of itself, the flexibility...everyone involved in the process. COVID-19 has changed many aspects of academia, and the PhD application...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014; Duboue et al., 2022), as they... now connects MDH-focused CUREs at 50 schools, ranging from R1 institutions to community colleges, and...instructors, and provides opportunities for students to exchange ideas and results with peers and faculty at other...classroom.   References and Resources References Bangera, G., Brownell, S. 2014. Course-based undergraduate...

  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...10 closely related yeast strains. Adaptation to changing conditions - Organisms often need to express genes...conditions. With varied codon usage, an organism can change which proteins are highly expressed and which are...idea and has been used successfully in the past (Angov et al., 2008), but there are still many more features...bodies and is folding properly. References 1. Angov, Evelina, et al. "Heterologous protein expression...

  6. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...General Meeting seven other times I had subsequently changed fields and my cohort of friends and colleagues ...in Atlanta, GA, in 2018. A lot of my experiences rang true with theirs.  It was at this dinner and the...Singletons logo. Image: James et al., 2020. Help me stranger - Finding mentors for the meeting Just like analysis... cells regulate gene expression in response to changes in cell density. At the mixer, they meet other ...

  7. Lab to Office Culture Shock

    Type
    Blog Post
    ...experience will be a little different. Some of these changes you may enjoy immediately and some of them may ... just take some getting used to. Just remember: change is good. You can experience a real onboarding...re also getting the opportunity to learn their “language”, and in doing so you may discover that your career...eventually. If you approach that transition dreading the change, the adjustment period will be that much longer...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ... has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart 1998). FLPe’s performance was further... single cell with all its synapses and how it rearranges these synapses in the living brain over time ...Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping.” Journal of Neuroscience 28(28)...Central PMCID: PMC2593125. 2. Buchholz, F, P O Angrand, and A. Francis Stewart. 1998. “Improved Properties...

  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...DNA 5' TTN Specific DSB in target DNA with 5' overhangs Cas13a (C2c2) 2x HEPN crRNA RNA 3' A, U, or... Cas13a targeting with a single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a...and cleavage through a series of conformational changes in the Cas13a molecule. Like Cas9, Cas13a tolerates...Ladha et al., 2020). Cas13a in mammalian cells The Zhang lab made the exciting discovery that some Cas13a...

  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... often weren’t easily accessible, due to a wide range of logistical barriers. Addgene worked to identify...availability vs accessibility issue was found in a less tangible form: knowledge and information about the materials...is achieved through interconnected incremental changes. It was only after creating a plasmid repository...learned about the importance of smaller, systemic changes, like using alt-text, captions, and inclusive design...

  11. Hot Plasmids - November 2023

    Type
    Blog Post
    ...reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (Delivery...Design). This modular approach combines an interchangeable set of fusogenic and targeting components to...where the fusion and targeting components are interchangeable. Image from Strebinger et al., 2023, reused...be tethered on the viral surface, expanding the range of targetable cell types. DIRECTED is compatible...

  12. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...skipped or whether selecting colonies and performing Sanger sequencing will suffice…and the answer is no. Due...screening - An excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production...CRISPR/Cas Systems Forum- Moderated by the Feng Zhang lab, this is a useful place to ask experimental ...Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional...

  13. The Challenges of Cell Culture

    Type
    Blog Post
    ...you to track a cell line’s age and monitor any changes. Your tracking system could be as simple as a piece... stocks to work from. The future Attitudes are changing but yet more is needed to convince people of some... to carry our independent quality control for a range of testing, from animal doping to forensics, DNA...Delivery Systems for Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources...

  14. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ... Today in our transferable skills guide: Time Mangement for Scientists. Back when I used to do mammalian...immediate deadline - even the best of friends get hangry at some point. My cells, on the other hand, could...terrible handwriting, is copied below): Read new Zhang Lab Paper Find plasmids similar to those in the ...while I may have found splitting cells boring, hanging out in the tissue culture room also gave me time...

  15. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...References Hao, Y., Yan, J., Fraser, C., Jiang, A., Anuganti, M., Zhang, R., Lloyd, K., Jardine, J., Coppola...reagents, researchers can measure this conformational change to investigate the role of specific integrins in...reagents to study the difference in cell morphology changes induced by integrins binding the RGD motif compared...

  16. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...institution, you can order plasmids covering a wide range of fields from Addgene. What you might not know ... Academy of Sciences 113.9 (2016): 2388-2393. Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III...et al 2015[2]). The proteins encoded in this set range in size from 4 to 156 kDa, enabling the user to ...5373-5383. Walkiewicz, Katarzyna, et al. "Small changes in enzyme function can lead to surprisingly large...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...that certain polymerases such as Taq generate A-overhangs (a feature used in TA-cloning). Such non-complementary...near the 5’-end) and that also generates 4 bp overhangs. You can then use the Klenow fragment of DNA polymerase...polymerase I to generate blunt ends from these overhangs and ligate these blunt ends together in a standard...Xia, Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase...

  18. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...caused by splicing defects, including ataxia telangiectasia, congenital disorder of glycosylation, and ...RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach CA. In vivo genome editing improves...Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers AJ. In vivo...Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. In vivo genome editing using Staphylococcus aureus...

  19. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...research Researchers can incorporate HiBiT with a wide range of workflows, from classic overexpression to CRISPR... these challenging proteins. VEGF-A mediates angiogenesis through its principal receptor tyrosine kinase...binding sites and various isoforms led to subtle changes in co-receptor dynamics, including NanoBRET assays...with high throughput processing and enables a wide range of detection capabilities. See a full list of detection...

  20. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...added, it binds tTA, resulting in a confirmation change that prevents binding to the promoter and turning...added, it binds cTA, resulting in a confirmation change that prevents binding to the promoter and turning...acid to Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also.... Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling gene expression with the Q repressible...
Showing: 321 - 340 of 843 results