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We narrowed to 490 results for: ARA-2

Showing: 321 - 340 of 490 results

  1. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...small-molecule fluorescent labels. Nature Methods, 19(2), 149–158. https://doi.org/10.1038/s41592-021-01338...help. Here, we will outline some fundamental characteristics to consider when selecting a dye or ligand ...Fluorescence microscopy takes advantage of the characteristics of these fluorophores to enable highly specific...receptor conformation and signaling activity​​ (Sridharan et al., 2014). It's also crucial to evaluate how...impacted by both the excitation coefficient, characterizing the strength a species absorbs or reflects ...considerations regarding the choice of fluorescent dyes (Sridharan et al., 2014). Manufacturers will typically recommend...

  2. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont...facilities producing a large number of viral preparations in parallel, there is a very real risk of mixing up...Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus...confirm the identity and serotype of our AAV preparations. We recently published a description of the ... For labs that produce a small number of AAV preparations this level of quality control is sufficient ...AAVs and the TA-based ligation and tagmentation preparation approaches described previously require a preliminary...effective substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...

  3. Editor's Choice, September 2016

    Type
    Blog Post
    ... of my favorite molecular biology tool, P1 phage. 2) The post describes an ingenious but simple technique...That’s it for now. As I indicated in the opening paragraph, I’m currently on my way back from a very successful...

  4. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...method relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity...are also known to trigger seed germination of parasitic plants, this approach was also used to build and...ratiometric fluorescent biosensor based on the parasitic Striga hermonthica SL receptor HTL7. This novel...compounds that have the potential to regulate the parasitization of cereal crops. As a result, agricultural ...

  5. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...in just 2 years and excitement about how much more there is left to do with the characterization and implementation...relatively isolated resorts creates a sense of camaraderie with fellow attendees from the moment you arrive...motto for scientists who contact us and want a guarantee that their experiments will work (yes, this happens...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...thanks to our guest blogger Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate at...post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University... level of progression, anatomical location, characteristic (solid localized tumors or systemic tumors)...

  7. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...PMID: 20161833. PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding...), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural changes...fluorophores, it’s best to consider these cases separately when talking about the tools that are available...tools for labeling reagents and structurally characterizing large ion channels involved in skeletal muscle...40.3 (2006): 279-286. PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach...

  8. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input concentration across ...many labs. However, the high costs of sample preparation for high-throughput next generation (NGS) sequencing...Addgene to develop and apply our plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation...of barcoded fragments is known as a *library*. Parallel sequencing of the fragments within a library allows...understated problem in multiplex NGS library preparation. The challenge here is that, as more samples ...minimize these issues, researchers usually include a separate normalization step where individual input DNA ...

  9. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...PMID: 25386923. PubMed Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary T. Britton, and Anne S...number of disulfide bonds in your protein - two parameters that affect how well E. coli can produce your...

  10. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...access this tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence...complement sequence.  Filter DNA tool: Removes non-DNA characters, such as numbers and blank spaces, from the text... primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly Design (DAD): ...

  11. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: 10.1038/nrd.2016.238 Additional resources...drugs. While we often think of these two fields as separate, they are deeply intertwined. In this blog we ...Target identification Not all diseases are fully characterized or have a simple pathology. In these cases, ...

  12. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...gotten easier now that I identify with the main character in the video. We’ve grown attached to the work...“WOW, now I have my own laboratory” (the main character is much more than a piece of plastic for us now...

  13. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...complicated entities with a large range of characteristics (color, brightness, photostability, maturation...attach important references that serve to further characterize a protein of interest (and we very much appreciate...simplifies the process of building advanced multi-parametric search queries.  Looking for a bright, monomeric...emission peak around 600nm?  Simply input these parameters and you’ll find matching FP suggestions. If ...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...posts: Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse...is less predictable. In our best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA... a mouse model, and its success is not always guaranteed. To learn more about this process check out our...

  16. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication...optogenetics. Even though GFP has been so thoroughly characterized, it turns out this protein has a few more secrets...

  17. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Genome Editing and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: Homology Directed...but with far fewer off-target effects! This characteristic probably explains why pC016 - LwCas13a guide...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...production phase. However, this has not been well characterized as it is not a common event. What are exosome...exo-AAVs rely on differential centrifugation steps to separate cell culture supernatant containing exo-AVVs from... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...29296848. PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of Exosome-Associated...2018): 293-305. PubMed PMID: 29767307.  Wassmer, Sarah J., et al. "Exosome-associated AAV2 vector mediates...

  20. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color fluorescent imaging! ...expressed in cells, generates much less signal than comparable fluorescent proteins. This weak emission hasn...! These eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine...Nagai laboratory has a done a wonderful job characterizing Nano-lanterns and eNL for many different applications...Nano-lanterns requires a linear unmixing algorithm to separate the various colors. In fluorescent imaging, you.... Filtering the emission wavelength partially separates the signals of various Nano-lanterns, but it does...
Showing: 321 - 340 of 490 results