We narrowed to 648 results for: ATO
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TypeBlog Post...Microbiology, National Emerging Infectious Disease Laboratories. She is interested in genome replication and...Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is...
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The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood. Work... -
Hot Plasmids: Fall 2024
TypeBlog Post...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...own replication machinery such as Rep or other initiator proteins, they do not require host proteins for... -
Hot Plasmids - November 2023
TypeBlog Post...example, neurons treated with a phorbol ester activator gave fluorescent signal in the ticker tape from...transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration can often be seen by... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus 5-derived...usually provided by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan.... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...signal inside the tumors was overlaid with the anatomical B-mode images in greyscale. ARG expression...-specific ultrasound signal is in hot and the anatomical B-mode images are in greyscale. (scalebar = 2mm... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar and Marty, Jean... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide... “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...versatility and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this.... References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala to nucleus accumbens... -
Tips for Getting a Faculty Position
TypeBlog Post...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...Livet, Jean, et al. "Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...liver progenitors in mouse oval cell injury." Hepatology 60.1 (2014): 278-289. PubMed PMID: 24700457. ... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...line and ~8 and 9% efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers ... -
March for Science
TypeBlog Post...know is your area code. Senators: To find contact information for your senators all you need to do is select... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...Often, however, researchers lack knowledge of the regulatory kinase, access to active kinase for the reaction...