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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 710 results for: BASE;

Showing: 321 - 340 of 710 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...unconjugated and recommended for use in flow cytometry. Based on the established applications of the original ...with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. Caitlin...

  2. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want to select a ... Off-targets that come from mutations arise from base/prime editing or Cas cleavage. In the case of Cas...Resources   References Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas...

  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... optogenetic tools for manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage...interested in directed-evolution methods, the activity-based selection in mammalian cells used here could be ...Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, ...powerful high-throughput screen to select variants based on performance metrics specifically for two-photon...phosphorylated state of an immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM to an RTK...

  5. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... The HA frankenbody works just like an antibody-based probe, but is easier and cheaper to generate since...scFvs: Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic...blog: Read about frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about ...

  6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ..., especially in in vivo models, and new Cre-lox based technologies are still being developed today. In...Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase...-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...conduct their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes...Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber...changes cytidines or guanines to the other three bases, which limits the level of variation that can be...

  10. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...commonly used for the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan...Assays to Assess Immunogenicity Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https...

  12. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...to altered function or maybe your favorite FRET-based biosensing technique can be applied to an important...experiments enabled by physical, software, and biology-based tools as well as collaborations between different...

  13. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian..." mammalian ORIs, scientists have usurped viral-based ORIs to fill the void. These ORIs, however, require...

  14. Antibody Production

    Type
    Collection
    ...determines ideal shipping conditions for each order based on the total items in the order, ensuring each item...

  16. DNA Quantification

    Type
    Protocol
    ...accurately measure the concentration of a substance based on its absorbance, you need to know the wavelength...

  18. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...by gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments ...you can assemble the different parts of a plasmid based on the cloning strategy you followed. While most...

  19. Summer SciComm: Preprints

    Type
    Blog Post
    ...critiques or endorsements. In contrast, journal-based peer review is usually a confidential process among...ensure a manuscript’s quality. But even journal-based peer review can be prone to biases or errors, with...

  20. Antibodies 101: Isotypes

    Type
    Blog Post
    ...staining. Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of two. There are four IgG subclasses, numbered based on their abundance in blood: IgG1, IgG2, IgG3, and...
Showing: 321 - 340 of 710 results