We narrowed to 382 results for: BRE

1. Getting to Know Expansion Microscopy

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   Blog Post

   ...SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing...

2. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ...employer. Your heart is pounding, you’re short of breath, and you’re a little jumpy. You are at another ...

3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...Zhen, et al. "Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes...

4. AAVs in Retinal Gene Therapy

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   .... Jean Bennett (UPenn) that resulted in the groundbreaking 2001 study showing that AAV-mediated delivery...

5. Plasmids 101: Colony PCR

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   ...is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR...

6. Tips for Screening with Yeast Two Hybrid Systems

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   ...Science. 1986; 231:699-704. PubMed PMID: 3080805. 3. Brent, R., and Ptashne, M. A eukaryotic transcriptional...

7. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   Blog Post

   ...facility). The third tube is used to fulfill orders. Breaking the (bar)code At each step in the receiving, transforming...

8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...studies, act as a reporter for DNA double strand break repair, and to label pathogenic intracellular microbes...

9. Your Lentiviral Plasmid FAQs Answered

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   ... insert encoded by the vector." The table below breaks down the biosafety concerns associated with various...

10. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ...exception is that men cannot actually give birth and breastfeed. Keeping this always at the forefront will go...

11. CRISPR Guide

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    Guide

    ...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...small precision edits. NHEJ directly ligates the break ends without the need for a homologous template.... experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments...Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation. This...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...conversion in RNA or DNA without a DNA double-strand break Cas C RISPR As sociated Protein, includes nucleases...

12. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...engineering single genome edits but the double strand breaks it introduces makes it difficult to perform two...

13. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    ...-1  Pennacchio, L. A., Bickmore, W., Dean, A., Nobrega, M. A., & Bejerano, G. (2013). Enhancers: five ...

14. CRISPR 101: Any Base Transversion Editors

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    ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...

15. Hot Plasmids: Fall 2024

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    .... abscessus. A) Sth1Cas9 mediates double strand break at the target site, which results in either bacterial...

16. Plasmids 101: Stringent Regulation of Replication

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    ...strands. Created with BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together...

17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ... Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation of “...

18. Finding nucleic acids with SHERLOCK and DETECTR

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    ... generates 5’ and 3’ staggered ends after dsDNA breaks. The Doudna lab discovered Cas12a’s ability to ...

19. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...matching Mentors with Singletons for short coffee breaks. While this was not the more intense mentorship...

20. An Inside Look into Addgene's Dev Team

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    ...collaborative approach to development. Scrum teams plan and break up work into small, well defined chunks, called ...