...primarily been performed in activated T cells, precluding analyses of T cell differentiation beginning ...also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that enables introduction...

... that the new system could also be expanded to include even more loci. Figure 1: Schematic overview... validated and ready. “Currently, the kit only includes two versions of Cas9, nuclease and nickase, but...

...research in synthetic biology and bioengineering including a recent effort to construct a synthetic yeast...: Research projects underway in the Ellis lab including yeast and E. coli engineering, microbial cooperation...

...specific blockers, and the manufacturer will usually include recommended blocking conditions on the spec sheet...especially important for highly expressed proteins, including “housekeeping” or ubiquitously expressed proteins... to improve binding and/or reduce background, including trying longer incubations at both temperatures...

...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...this agent to increase transfection success.  In conclusion, it is important for you to establish in advance...

... Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ...

...you run a restriction digest on a gel you always include proper controls like uncut DNA and the proper ladder...amplification reaction (as opposed to using the all inclusive service some companies offer), you can purify ...

...engineering research. A variety of new tools came out including the single base editors, Casilio, CombiGEM, and...2A-Puro (PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features...

...organization runs a wide variety of programs, including fall and spring lecture series, science cafes,... information about SITN: DayCon event website (includes archived content) Science in the News website ...

...Start something (a journal, a science lunch club, a biotech club) Be the head of a group (like a grad student...

...that introduced the kit in December, GreenGate includes the six most frequently used elements in plant...works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and hope that other ...

... lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids...years after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...

...over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade et al, 2016...is both sortable and searchable, which can help exclude the data that you are not interested in. You can...

...on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee ... variety of genome modifications in S. Pombe, including full and partial gene deletion, overexpression...

...page, and a lot of the content was either small, cluttered, or hard to find. This made the page look not ... place all of the content above the fold, we decluttered the page elements and allowed our content to ...

...scientists in 88 countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 ...it more accessible. Other accessibility updates include a printer-friendly design and alt text on all images...

...mentoring and applauded the new requirement for PIs to include in their annual NIH progress reports information... research be? The second day of the conference included workshops which were framed in advance by the ...

...met the astronaut Jim Lovell I was speechless (the club of people who have been in space is pretty small...Doudna has had a host of other impressive mentors including Tom Cech, Robert Tijian, Tom Steitz and Joan Steitz...

...colleagues built a Molecular Expressions collection including some 3,300 plasmids along with image galleries...specific cellular structures.The Davidson lab even included some of their own reference images that can be...

...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA, dopamine,...