We narrowed to 802 results for: Clu
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TypeBlog Post... there! Completed blog posts are also great to include on CVs and Resumes. An Expanded Network - Addgene...many different people. Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen ...
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Our New Antibody Mascot is....
TypeBlog Post...hunts. In fact, they’ve been following a set of clues that has led them to their biggest discovery yet...name! Additionally, anyone whose submission is included in the final four will receive some rockin’ newly... -
Performing In Vivo CRISPR Screens Using the FITS Approach
TypeBlog Post...primarily been performed in activated T cells, precluding analyses of T cell differentiation beginning ...also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that enables introduction... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post... that the new system could also be expanded to include even more loci. Figure 1: Schematic overview... validated and ready. “Currently, the kit only includes two versions of Cas9, nuclease and nickase, but... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog Post...research in synthetic biology and bioengineering including a recent effort to construct a synthetic yeast...: Research projects underway in the Ellis lab including yeast and E. coli engineering, microbial cooperation... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...specific blockers, and the manufacturer will usually include recommended blocking conditions on the spec sheet...especially important for highly expressed proteins, including “housekeeping” or ubiquitously expressed proteins... to improve binding and/or reduce background, including trying longer incubations at both temperatures... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post... Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...other uses. Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...this agent to increase transfection success. In conclusion, it is important for you to establish in advance... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post...you run a restriction digest on a gel you always include proper controls like uncut DNA and the proper ladder...amplification reaction (as opposed to using the all inclusive service some companies offer), you can purify ... -
Your Top Requested Plasmid in 2016!
TypeBlog Post...engineering research. A variety of new tools came out including the single base editors, Casilio, CombiGEM, and...2A-Puro (PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features... -
Science Communication Snapshot: DayCon 2015
TypeBlog Post...organization runs a wide variety of programs, including fall and spring lecture series, science cafes,... information about SITN: DayCon event website (includes archived content) Science in the News website ... -
Developing Transferable Skills During Science Training
TypeBlog Post...Start something (a journal, a science lunch club, a biotech club) Be the head of a group (like a grad student... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...that introduced the kit in December, GreenGate includes the six most frequently used elements in plant...works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and hope that other ... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post... lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids...years after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade et al, 2016...is both sortable and searchable, which can help exclude the data that you are not interested in. You can... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee ... variety of genome modifications in S. Pombe, including full and partial gene deletion, overexpression... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...scientists in 88 countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 ...it more accessible. Other accessibility updates include a printer-friendly design and alt text on all images... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...page, and a lot of the content was either small, cluttered, or hard to find. This made the page look not ... place all of the content above the fold, we decluttered the page elements and allowed our content to ... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...mentoring and applauded the new requirement for PIs to include in their annual NIH progress reports information... research be? The second day of the conference included workshops which were framed in advance by the ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...met the astronaut Jim Lovell I was speechless (the club of people who have been in space is pretty small...Doudna has had a host of other impressive mentors including Tom Cech, Robert Tijian, Tom Steitz and Joan Steitz...