...released a major redesign of how search works on our site. For Addgenies, development includes everything from...experience and the overall soundness of the software design. Each of us brings slightly different cognitive...Addgene. Another advantage of Scrum is that it provides transparency to everyone involved about the status... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...our users, we are able to make informed software design choices, resulting in software that helps our users...

...site, and titer. Addgene’s Viral Vector Service Besides being complex to use, viral vectors are also difficult...which include calcium and voltage sensors, are the broadest category of viral vector tools, which may help... for vectors in the “controls” category, which includes things like viral vectors that deliver GFP. ...most requested serotypes (Figure 4), our catalog includes vectors in a variety of serotypes, including the...early engagement with UPenn to our launch of the redesigned Data Hub in 2022. It’s influenced the way we ...viral vector educational resources, from eBooks to guides to protocols, which was why I was so excited to...

...of data. In this two-part blog series, we will describe the importance of bioinformatics at Addgene and...each week. Our internal software was originally designed to help us work in large formats, such as 96-well...Thanks to the flexibility inherent in Nextflow’s design, we were free to use multiple programming languages...alone! The benefits of containers are they are designed to ‘contain,’ in a complete package, all of the... who or which computer ran the process. Another design choice we appreciated about Nextflow was how effortless...small with a print statement and tested that the desired data was available so we felt confident we were...

...'s repository contains 158,197 plasmids, which includes plasmid collections from depositors. To help you...Dehydrogenase CUREs Community (MCC) Collection Pleiades Promoter Project: mini-promoters to drive selective...

...Bioworks (Link opens in a new window) used their DNA design and synthesis capabilities, in collaboration with...Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Resources ...

...scarless mutations, ones that don’t leave any undesired scars in the chromosome. These methods roughly... recombination enzymes can also accomplish the desired genomic modification. This technique is frequently...resolution step (“double crossover”) which leads to the desired mutation and excision of the vector backbone. This...the pDS132 MCS with a versatile, synthetic MCS (designed with the help of the DNA tuner program) (Latynski...purified amplicons upstream and downstream of the desired deletion directly into cut vector with excellent... of interest, from the day primers arrive, the desired chromosomal modification can be made in 5 days....

...site-specific incorporation of phosphoserine (as described below) provides researchers with a valuable tool for understanding...organisms lacked a classic cysteinyl tRNA synthetase, despite abundant incorporation of cysteine in their proteomes...pSer-incorporated proteins on a single plasmid. This includes 4 copies of a tRNA that specifically recognizes...context-specific effects of UAG suppression, as described (6). To determine the stoichiometry of phospho-incorporated...phospho:non-phospho species on PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid...

...Wilpitz founder and lead consutant at Experiment Designs Consulting, Inc. “How much do you need [for your...necessary resources. This is why at Experimental Designs Consulting (EDC), we instead have our clients focus...the institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and... as many of these tools as possible, this even includes clocks, timers, and phones.  As for reagents and...passion for science. Follow him on Twitter @XpDesignsMGNT        Additional Resources Find other career...the Addgene Blog! Learn more about Experimental Designs Consulting at their website. ...

...R26R-Confetti described in Hugo J. Snippert, et al. (2010) and the MAGIC Marker strategy described in Karine...Brainbow-1 system employs Cre-mediated deletion, the design of Brainbow-2.1 cleverly combines Cre-mediated ...protein sequences are highly homologous, preventing design of antibodies specific to each fluorophore. Third...immediately following the promoter. The STOP cassette includes a mutant YFP that does not fluoresce, but can ...produces a minimum of 8 colors. The use of AAV provides spatial and temporal control without the need ...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive...easy to implement due to the simplicity of gRNA design, programmable for different genomic loci, capable...

...We're excited to roll out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place [email protected] to learn more. A great submission includes: Images that clearly show viral spread and cellular...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...work. Sharing your negative data helps others to design better experiments (and wouldn't it be nice if ...

...researchers at Japan’s Hiroshima University who described their new tool in Scientific Reports in June. ... gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study, the construction system...vector-mediated multiplex genome engineering. The system now provides an efficient targeting strategy for multiplex ... validated and ready. “Currently, the kit only includes two versions of Cas9, nuclease and nickase, but...Addgene here. Other Popular CRISPR Posts: How to Design Your gRNA for CRISPR Genome Editing CRISPR-Cas9...

...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying molecules associated...interest in vivo. For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific...

...Localization Determine where your protein of interest resides by using a well-characterized fluorescent fusion... Medical School Fluorescent Biosensor Database designed by Eric Greenwald and the Jin Zhang Lab at UCSD...

...antibody worked under the conditions described above, and a short description of how the antibody performed....the submitted application, along with a brief description of the experiment. If that data was included ...ELISA — but in all cases, it contains a detailed description of the experimental conditions.     Figure 4...The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate, and...

...colleagues describe in the PLoS ONE paper that introduced the kit in December, GreenGate includes the six... bring this into your target organism. We have designed a Golden Gate system based on the daily needs ...and cut another,” Lohmann said. “It allows us to design modules that always have the same recognition site...produced will have different overhangs, they can be designed such that they go back together only one way and...

...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin... light in combination with retinal) variations designed to give researchers flexibility in using optogenetic...precise timing of the perturbation. Other tools are designed to be activated within a narrower band of wavelengths...function through multiple intra-cellular signaling cascades. Optical methods for controlling GPCR activation...control GPCR activation and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including ...You’ll need to test for these differences when designing your experiment.  Finding the right information...

... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...through our in-stock viral prep service.  AAV2 Despite being isolated second, AAV2 is the best studied...experimental reports on commonly used vector cargo designs and administration doses. Don't forget to submit...2008). Adeno-Associated Virus (AAV) Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1,...., Schaffer, D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient Retrograde Access...., Robbins, P. D., Li, J., & Xiao, X. (2006). Widespread and Stable Pancreatic Gene Transfer by Adeno-...

...larger Antibody Plasmid Collection , which also includes other types of affinity reagents such as scFvs...Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype Reactivity Recommended...

...Plasmids Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses...cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning applications. Direct transfection...