We narrowed to 443 results for: DUR

1. Transferable Skills Guide: Managing a Team

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   Blog Post

   ...mine you didn’t think much about management skills during your scientific training. I never thought about...

2. 3D Printing Meets CRISPR Cas9

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   ...their mental model of the protein. At some point during this interrogation process, they will almost invariably...

3. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...layered and then the viral suspension generated during AAV production is overlaid. Following ultracentrifugation...

4. Google Forums Round Up: First Impressions of NgAgo

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   ...protein to load an ssDNA guide. It is possible that, during translation, only a small fraction of nascent NgAgo...

5. Plasmids 101: Optimizing Plasmid Yields

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   ...favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than...

6. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...mutations seen with TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and...

7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   ...there are many factors that need to be considered during the planning process. For CRISPR experiments, the...

8. Plasmids 101: Multicistronic Vectors

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   ...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988...

9. An Introduction to Adenovirus

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   ...’ functions are rescued by helper plasmids used during production and/or by genes present in the HEK293T...

10. Course-Based Undergraduate Research Experiences (CUREs)

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    ...dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school. As a stable, easily purified protein...

11. MXS Chaining

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    ...various mammalian promoters), the same cloning procedure can be used to incorporate each element into the...

12. Tips for arabidopsis transformation

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    ...al., 2006. Tips: Don’t plate seeds too densely during selection: this will increase background growth...

13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    .... For transposons to be specifically recognized during transposition, genetic cargos must be flanked by...

14. Negative Can Be Positive: Open AAV Data with Addgene

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    ...aggregation and methods to prevent its occurrence during vector purification and formulation. Molecular ...

15. Plasmids 101: Colony PCR

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    ...to the PCR reaction. The bacteria will be lysed during the initial heating step of the PCR reaction. A...

16. Tips for Screening with Yeast Two Hybrid Systems

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    ...eukaryotic transcriptional activation and cell biology. During this period, proteins were successfully expressed...

17. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...tubes help to guarantee that no samples get lost during their journey. Take the time to track your samples...

18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...activity – it only requires the presence of oxygen during maturation. This meant that the protein could be...

19. Hot Plasmids: Fall 2024

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    ...faster, with expression levels comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3 also...

20. Plasmids 101: Stringent Regulation of Replication

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    ...lab, low copy plasmids may result in low yields during DNA purification. On the other hand, they also ...