We narrowed to 445 results for: DUR

...their mental model of the protein. At some point during this interrogation process, they will almost invariably...

... you can always change your compensation values during the analysis portion of your experiment. So, as...

...the usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR ...

...protein to load an ssDNA guide. It is possible that, during translation, only a small fraction of nascent NgAgo...

...favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than...

...mutations seen with TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and...

...there are many factors that need to be considered during the planning process. For CRISPR experiments, the...

...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988...

...aggregation and methods to prevent its occurrence during vector purification and formulation. Molecular ...

.... For transposons to be specifically recognized during transposition, genetic cargos must be flanked by...

...various mammalian promoters), the same cloning procedure can be used to incorporate each element into the...

...’ functions are rescued by helper plasmids used during production and/or by genes present in the HEK293T...

...al., 2006. Tips: Don’t plate seeds too densely during selection: this will increase background growth...

...dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school. As a stable, easily purified protein...

...to the PCR reaction. The bacteria will be lysed during the initial heating step of the PCR reaction. A...

...eukaryotic transcriptional activation and cell biology. During this period, proteins were successfully expressed...

...tubes help to guarantee that no samples get lost during their journey. Take the time to track your samples...

...activity – it only requires the presence of oxygen during maturation. This meant that the protein could be...

...methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and...

...of translation. The ribosome may slow and pause during elongation which may actually be necessary for ...