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  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the two breakpoints...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988...

  7. MXS Chaining

    Type
    Blog Post
    ...various mammalian promoters), the same cloning procedure can be used to incorporate each element into the...

  9. An Introduction to Adenovirus

    Type
    Blog Post
    ...’ functions are rescued by helper plasmids used during production and/or by genes present in the HEK293T...

  13. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...to the PCR reaction. The bacteria will be lysed during the initial heating step of the PCR reaction. A...

  19. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ..., and it is exchanged with the gene of interest during the BP or LR reactions. Since the CcdB protein ...
Showing: 321 - 340 of 441 results