We narrowed to 753 results for: ELP
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TypeBlog Post...current methods, understanding the differences can help you make an informed decision. Mammalian cells Mammalian...on these differences in viral production and will help you decide which is right for you. Table 1: Mammalian...
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Magnetic Control of Proteins: More than a Dream
TypeBlog Post...they have collected a variety of data that might help formulate some hypotheses. They are excited to see...troubleshooting tips and works with other labs to help them adopt the tools his group develops. And though... -
Pooled Library Amplifications
TypeBlog Post...is your desired library coverage, since this will help decide what transformation efficiency you need to...plates). In general, amplification on plates can help to ensure that diversity of the library is maintained... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post... CRISPR experiments, the following framework can help get you started: Determine what type of outcome...domains to control gene expression. Base editors can help you make precise point mutations near the gRNA target... -
DNA Purification Without a Kit
TypeBlog Post...fragments You might be wondering, “How is milk going to help me extract DNA from agarose gel?!” The DNA gel... it solubilizes both DNA and agarose, and 2) it helps DNA stick to the glass. After binding DNA, the glass... -
AAVs in Retinal Gene Therapy
TypeBlog Post...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy...and colleagues at MEEI co-founded a non-profit to help push forward candidate therapies for these diseases... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...genome-wide gain of function screens. These screens can help reveal the function of lowly expressed genes or ...with two MS2 RNA aptamers, and the MS2-P65-HSF1 helper protein (Konermann et al., 2015). Root and Doench... -
Getting to Know Expansion Microscopy
TypeBlog Post...density. In some cases, nanobodies have been found to help improve label density (Mikhaylova, et al. 2015)....approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...improvements to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as ...for the modification in the assay host strain can help. In order to reduce the chance that the configuration... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...activities because I can contribute by, for example, helping to improve the papers submitted to journals. In...Japan, which is very disappointing. I hope I can help change the situation in the future. Addgene: What... -
Viral Vectors 101: Pseudotyping
TypeBlog Post... lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using...stable and can withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...For us, transient leaf transformations are a great help in screening candidate genes and speeding up research...transformations, but we hope that these tips might help to get you started. Once you have established a ... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...reliable results from CRISPR edit data requires the help of robust software tools. As a consequence, a critical...detected edit types in the sample. The visualization helps the researcher see which of the multiplexed sgRNA... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...RepZ to drive its own replication. Like RepA, RepZ helps recruit host initiation proteins to the plasmid ...Plasmids 101 Posts Get practical molecular biology help with our Molecular Biology Reference Pages ... -
How to Write a Scientific Review Article
TypeBlog Post...stuck trying to write one, hopefully this post will help you get things moving - remember you're becoming...Year) in the document while writing, because it helps you later on to remember where you read particular... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...preventive measure designed to help keep you from getting sick can be helpful in preventing the spread of... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...gene modified mouse lines. Hopefully this post will help get your in vivo research up and running as soon...Resources on Addgene.org Find Software Tools to Help Design You gRNA Find CRISPR Plasmids for Use in ... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post... that receive different types of stimulation may help to refine and optimize your stimulation parameters...Happy experimenting and hopefully optogenetics will help illuminate (is that pun worn out yet?) your research... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...are a few simple troubleshooting measures that can help get you back on track: Compatibility: Double-check...inducer or adding an affinity tag such as GST may help with solubility issues. We want to hear about your... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...SlideShare, Figshare and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload the...their data. Likewise, we want to contribute to helping others understand ours. Improving access to data...