We narrowed to 755 results for: ELP

...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult...Exploring the efficiency of these two processes will help us better understand where Cpf1 fits into the genome...

...learn all about ddPCR for AAV titration and find helpful tips and tricks in our blog post “Droplet Digital... This is an extremely useful PCR method that can help save time and effort in the laboratory.  There are...

...current methods, understanding the differences can help you make an informed decision. Mammalian cells Mammalian...on these differences in viral production and will help you decide which is right for you. Table 1: Mammalian...

...they have collected a variety of data that might help formulate some hypotheses. They are excited to see...troubleshooting tips and works with other labs to help them adopt the tools his group develops. And though...

...is your desired library coverage, since this will help decide what transformation efficiency you need to...plates). In general, amplification on plates can help to ensure that diversity of the library is maintained...

... CRISPR experiments, the following framework can help get you started: Determine what type of outcome...domains to control gene expression. Base editors can help you make precise point mutations near the gRNA target...

...density. In some cases, nanobodies have been found to help improve label density (Mikhaylova, et al. 2015)....approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal...

...fragments You might be wondering, “How is milk going to help me extract DNA from agarose gel?!”   The DNA gel... it solubilizes both DNA and agarose, and 2) it helps DNA stick to the glass. After binding DNA, the glass...

...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy...and colleagues at MEEI co-founded a non-profit to help push forward candidate therapies for these diseases...

...genome-wide gain of function screens. These screens can help reveal the function of lowly expressed genes or ...with two MS2 RNA aptamers, and the  MS2-P65-HSF1 helper protein (Konermann et al., 2015). Root and Doench...

...improvements to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H) system as ...for the modification in the assay host strain can help. In order to reduce the chance that the configuration...

...activities because I can contribute by, for example, helping to improve the papers submitted to journals. In...Japan, which is very disappointing. I hope I can help change the situation in the future. Addgene: What...

... lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using...stable and can withstand ultracentrifugation which helps scientists achieve a high titer (reviewed in Joglekar...

...reliable results from CRISPR edit data requires the help of robust software tools. As a consequence, a critical...detected edit types in the sample. The visualization helps the researcher see which of the multiplexed sgRNA...

...stuck trying to write one, hopefully this post will help you get things moving - remember you're becoming...Year) in the document while writing, because it helps you later on to remember where you read particular...

...For us, transient leaf transformations are a great help in screening candidate genes and speeding up research...transformations, but we hope that these tips might help to get you started. Once you have established a ...

...RepZ to drive its own replication. Like RepA, RepZ helps recruit host initiation proteins to the plasmid ...Plasmids 101 Posts Get practical molecular biology help with our Molecular Biology Reference Pages   ...

...preventive measure designed to help keep you from getting sick can be helpful in preventing the spread of...

...gene modified mouse lines. Hopefully this post will help get your in vivo research up and running as soon...Resources on Addgene.org Find Software Tools to Help Design You gRNA Find CRISPR Plasmids for Use in ...

... that receive different types of stimulation may help to refine and optimize your stimulation parameters...Happy experimenting and hopefully optogenetics will help illuminate (is that pun worn out yet?) your research...