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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 660 results for: EPO

Showing: 321 - 340 of 660 results

  1. Viral Production at Addgene

    Type
    Blog Post
    ...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... inactivated by the complement system in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping...–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL...

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...Things Lentivirus with our Lentiviral Guide Pages Deposit Your Lentiviral Vectors ...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either... well of your cells. Regardless, the amount of reporter protein activity correlates to both the amount...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

  7. Five Popular Model Organisms

    Type
    Blog Post
    ...discovery of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in ...important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed PMID: 28804628. PubMed...

  11. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...great point when I then ask “and who has deposited to the repository?” When most or all of the hands go down...

  13. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...distributed throughout the genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help... up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ...

  16. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ...

  17. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts...

  18. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

  20. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central...
Showing: 321 - 340 of 660 results