We narrowed to 642 results for: Emb;

...adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction Bunz Return to top Adenoviral...using the AdEasy™ System Content last reviewed: 12 November 2025 Do you have suggestions for other plasmids...

...between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028    Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label...

...proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693. PubMed PMID: 28926338. PubMed...Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed PMID: 29224777...

.... Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...nucleus. But if the inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized ...

...Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

..., the “Reproducibility for Everyone” team was assembled when it was realized that interactive workshops...connected, this diverse and international team assembled the right people at the right time. Be inclusive...

...going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different types ... professional life, let me know that, too. And remember, if you discover during grad school or during ...

...and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ...

...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as ...resources and protocols! We invite feedback from all members of the research community to help us develop these...

... lab members. We can set ourselves up for success by giving explicit instructions to lab members through...

...something that is actually useful to you. When embarking on a new literature search, try to find a recent... rudimentary, but it works for me. I can often remember which paper I need to open, but when I can’t it...

...they’ve gone along, Voigt’s group has also been assembling the toolbox needed for anyone to begin considering...papers, published in Molecular Systems Biology in November, Voigt and Alex Nielsen describe what’s possible...

...targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology...

...days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses...

...encoded by mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(...cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody and a species-specific...

...with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to...pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A ...

...laboratory experience is focused on classical embryology techniques such as basic observation and dissections... that will bind to complementary mRNA within an embryo or tissue. Once the riboprobes are created, the...

...have a half life of 84 hours in primary mouse embryonic fibroblasts. This fusion protein can be used to... The researchers tested several scenarios from embryo development to myeloid proliferation in acute myeloid...

...sensors available that will localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an ...the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References and Resources...

...TET, can, like the iron ball-and-chain that they resemble, actually impede your investigation into the regulation...CpG so that nothing else can interact with it. Remember, the goal of this experiment is to expose an unmethylated...