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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: GAN

Showing: 321 - 340 of 610 results

  2. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...the relevant tag and then introducing a specific ligand to mark the tag and whatever it is fused to for...expressed, protein levels will quickly recover once the ligand is removed. Degron tag systems include small molecule...

  3. All in a Twist: dsRNA

    Type
    Blog Post
    ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    ...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...

  7. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...viral services. After a month or so of meetings, I began to notice something: even though our scientists ...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...

  8. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

  9. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...

  10. Lab Automation at Addgene

    Type
    Blog Post
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...

  11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating fluorescent protein .../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources...

  13. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1...

  16. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...

  18. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which will...target protein and an E3 ligase through interacting ligands. After an E3 ligase facilitates ubiquitination ...

  19. Transferable Skills: Negotiation

    Type
    Blog Post
    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...
Showing: 321 - 340 of 610 results