We narrowed to 610 results for: GAN

1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ... binding domains fused to either the hER or hPR ligand binding domains and the MSN2 transactivation domain...Transcription factor activation via the appropriate ligand - estradiol for hER or progesterone for hPR - leads...

2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ...

3. Plasmids 101: The protein expression toolbox

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   ...the relevant tag and then introducing a specific ligand to mark the tag and whatever it is fused to for...expressed, protein levels will quickly recover once the ligand is removed. Degron tag systems include small molecule...

4. All in a Twist: dsRNA

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   ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...

5. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...understanding of the physiology and pathology of the basal ganglia in movement disorders.   References and Resources...protein-coupled receptors potently activated by an inert ligand. Proceedings of the National Academy of Sciences...

6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer...

7. Transferable Skills Guide: Cross-team Communication

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   ...viral services. After a month or so of meetings, I began to notice something: even though our scientists ...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...

8. The Future of Research Symposium Boston 2015

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   ...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

9. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   ...as inhibitory neurons, rods, cones, or retinal ganglion. Rather than using naturally occurring promoters...Cre-dependent-GFP expresses in horizontal cells and ganglion cells, only the horizontal cells transduced by...

10. Lab Automation at Addgene

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    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...

11. Making CRISPR Plasmids Using Fragmid

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    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...

12. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    ...you can accomplish this by employing a neat and organized format.  Columns. You can choose from either ...Mike Morrison, a psychology doctoral student at Michigan State University. The idea is that the main research...

13. Mouse Modeling, Part 1: Genetically Engineered Mice

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    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1...

14. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...work or volunteer for a science communication organisation like Building with Biology. Local environmental...You can draw live sketches at conferences like @ATJCagan or create agar art with colorful microbes? Try...

15. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating fluorescent protein .../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources...

16. Adapting Toehold Switches to Detect Zika Virus

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    ...Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...

17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    ...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ...

18. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...techniques: the powerful tools for spatial genome organization deciphering. Molecular Cytogenetics, 11. https...

19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...this bottleneck, we began building CrispyCrunch in July 2018 and in November, we began using it internally...

20. Transferable Skills: Negotiation

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    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...