Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,140 results for: Gene

Showing: 321 - 340 of 1140 results

  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...lines and are not used for general plasmid selection. Instead, reporter genes are often employed to easily... be assayed conveniently. A direct test for your gene of interest may be another method to assess transfection...conjunction with positive selection to ensure your gene gets targeted to a specific location within the ...used selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action...use G418. This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin...Read More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All Plasmids...the genetic traits of the foreign material and will subsequently pass it on to future generations. Stably...

  2. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...Imagine you’ve just discovered that your favorite gene was described in a CRISPR screen publication. You...actual data. Now you’re wondering if your favorite gene is included in other CRISPR screens and whether ...researchers to query all public CRISPR results for any gene of interest. In addition, users interested in a ... thresholds used by the authors to determine the gene hits for each screen are also captured as metadata...results are reported as the original quantitative gene-level scores provided by authors rather than sgRNA-level...available resource developed by the Biological General Repository for Interaction Datasets (BioGRID) in...Twitter at @biogrid.     Additional resources on the Addgene blog Read this blog post about genome-wide screening...

  3. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes are expressed under native...sequence and a selectable gene product for isolation of successful transformants. Addgene distributes several...be interested in attaching epitope tags to their genes of interest, allowing for easy capture and detection...modifications in S. Pombe, including full and partial gene deletion, overexpression (by promoter substitution...few. Have you used these tools in your own lab? Addgene would love to hear from you, our community, about...

  4. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...526) that (1) enables efficient knockout (KO) of genes in immune cells; (2) is not immunogenic, a necessity...for in vivo studies; (3) is compatible with single gene KO and pooled screening approaches; (4) encodes ... optimize conditions for a screen that assessed genes that regulate antigen-specific CD8+ T cell responses...screen, we used the pRDA_526 vector to knock out 30 genes putatively involved in CD8+ T cell responses to ...Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens...10.1084/jem.20230699. Additional resources on the Addgene blog X-CHIME: Context-Dependent Germline Knockout...

  5. Retrovirus Plasmids

    Type
    Collection
    ...your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...transfer plasmids. Want a retroviral plasmid with your gene of interest? Search our database for retroviral ...Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen for ...'s genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets integrated...to ∼8 kb), and infection results in long-term transgene expression. Some disadvantages of γ-retroviruses...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...REVeRT, for gene delivery using reconstitution via mRNA trans-splicing. By splitting a large gene into smaller...the introduction of extraneous genetic material to reconstitute the gene of interest, reducing the immunogenicity...hIBA1a, 466 bp) that drives high microglia-specific gene expression when incorporated in an AAV vector (Figure...doi.org/10.1101/2023.12.12.571321.  Revolutionizing Gene Therapy: The REVeRT Dual AAV Vector System By Vaibhav...AAVs, and then splicing their mRNAs, the functional gene product is reconstituted within the target cells...vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing both the mechanisms...trans-splicing dual AAV vectors for (epi)genome editing and gene therapy. Nat Commun., 14(1), 6578. https://doi.org...

  7. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...available at Addgene CRISPR-Cas9 References, Protocols, and more References: Generation of Gene-Modified ...the CRISPR/Cas9 system to precisely edit specific genes in monkeys. "Our study shows that the CRISPR/Cas9...system enables simultaneous disruption of two target genes in one step without producing off-target mutations...Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014...many human diseases are caused by genetic abnormalities, targeted genetic modification in monkeys is invaluable...invaluable for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline...

  8. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...zebrafish genes; many of these were generated as part of an NIH-sponsored effort to provide gene-editing...zebrafish genes. TALengineering... TALengineering Reagents TALEN Zebrafish Reagents TALENs for Endogenous Zebrafish Gene Targets...gene-editing nucleases to the zebrafish community. Target Gene Name TALENs Full Target Site (5' to 3'; half-sites...

  9. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...variations in gene expression, they found that for approximately 40% of the identified genes, the genes were not...explanation. Next, they hypothesized that differences in gene expression could explain why some ancestry groups...groups were differentially dependent on certain genes. This didn’t prove to be correct, either. However, ...sequences for sgRNAs that target ancestry-associated genes.   What does the reference genome actually reference...cases, this may lead researchers to deprioritize genes that could be good targets for therapeutic development...cells’ germline genetic variations. They then wanted to identify cancer-associated genetic dependencies ...ancestry which will reduce the likelihood of generating genetic dependency data with an ancestry-based bias...

  10. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...on vectors where the gene of interest is inserted into the middle of the lacZ gene, thus disrupting 𝛽-...where only cells that have gained or lost a specific gene survive (ex: antibiotic resistance marker). In a... the blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase which ...scientists have found that when a portion of the gene is deleted, it creates a non-functional 𝛽-galactosidase...mentioned above. In molecular biology, cloning a gene into your plasmid is often a numbers game. By using...troubleshooting Sanger sequencing results. At Addgene, we now use next-generation sequencing during our QC process....pigment. Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome...

  11. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...possible deletion strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located...evaluate the RNA, perform RT-qPCR for gene expression of the relevant gene 7,8 . To evaluate the protein, perform...genome editing tool that allows genetic perturbation of genes and genetic elements. Here we present a simple...for efficient loss-of-function studies of genes and genetic elements in mammalian cell lines. Protocol...theory and methods, perspectives for drug delivery, gene therapy and research. Gehl J. Acta Physiologica...Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present... be utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include...

  12. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...applications used to study gene function. From making transgenic animals, gene targeting and genome modifications...These vectors allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under...design. You can create a new strain with a disrupted gene by transforming wild-type yeast with an integrating...modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check... More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other... is which vector will best suit your needs? At Addgene, we have a vast collection of empty backbones that...vivo (try pcDNA3-EGFP). If you can get away with generating transiently transfected cells, you will not need...

  13. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...region of the gene they are trying to disrupt or delete, which both inactivates the gene and acts as a...maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once – it...Historically, antibiotics have also been used to disrupt genes at the chromosomal level. Scientists introduce an...typically has two separate resistance cassettes and your gene of interest is cloned into/inactivates or completely...resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit...lab, their mechanism for killing bacteria, and general working concentrations. For instructions on how...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the...

  14. What's New in CRISPR - May 2019

    Type
    Blog Post
    ... gRNAs) that targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs targeting...CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans was developed by ... transcription of HSP90 an essential C. albicans gene. Read the paper in mSphere Find the dCas9 and... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  15. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    ...Energy’s Joint Genome Institute for uncharacterized genes that were proximal to a CRISPR array and the universal...CRISPR-Cas systems containing cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40...-Cas systems. There are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these...potential of Cas14, the Doudna lab cloned the cas14 gene together with the adjacent CRISPR array and intergenic...presence of infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA specific of a viral...ID PMID: 30337455. Additional resources on the Addgene Blog Learn more about DETECTR Browse all of the...Download our CRISPR cheat sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ... are, in most cases, interested in transferring genes to mammalian cells (i.e. transferring DNA), yet ...infection; however this would not be great for a gene therapy vector! For viral vectors, only the protein... is (thankfully) gutted of many essential viral genes. How it is, exactly, that heaps of viral vector ... particles are made while missing the polymerase gene may seem like a mystery. But it’s simple. The RT...the only component that is not dependent on viral genes. It is derived from the host/producer cell membrane...full “life cycle” of expression of selfish, lazy genes of a non-living viral particle, and how we as dedicated...Kantor, B. (2021). Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State...

  17. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...Adeno-Associated Viral Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https...Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector dose–...dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038/s41434-018...Adeno-Associated Virus Serotype 9 (AAV9) Preparations. Human Gene Therapy Methods, 30(6), 214–225. https://doi.org...Resources on addgene.org  Download Addgene's Viral Vectors 101 e-book Additional Resources on the Addgene Blog... by Addgene in 2021 asking scientists what data would improve our AAV Data Hub.   The Addgene Data...

  18. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...dominant disease-causing gene, or that carry only one copy of a recessive gene. Ma et al. claim that CRISPR...Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org Browse All CRISPR Plasmids...authors to title the paper “Correction of a pathogenic gene mutation..” rather than using term ‘editing.’ While...but this advantage likely won’t transfer to all genes we’d like to target. In addition, as on-target editing...Reference Ma, Hong, et al. "Correction of a pathogenic gene mutation in human embryos." Nature. Epub 02 August...hypertrophic cardiomyopathy and other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos...PubMed PMID: 28783728. Additional Resources on the Addgene Blog Check out Our New CRISPR Topic Page Read ...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...detect gene copy number based on the number of fluorescent puncta observed. By labeling two genes of a ...vivo. Catalytically dead Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting...Chen et al. successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the...also monitor the spatial relationship of the two genes over time, at a range of distances from 2-75 Mbp... envision using CRISPRainbow in combination with gene editing. CRISPRainbow requires very short, 11-mer...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Read More about Fluorescent...cells! This technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to...

  20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...functionally characterize combinatorial gene knockouts generated via multiplexed gRNA expression (Wong ...et al., 2014; Konermann et al., 2014) individual genes for studies interrogating their functions. The continual...list of effective gRNA sequences targeting your genes of interest. Thanks to the tremendous efforts made... et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 (2014): 647... "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell ...Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-virus-induced cell death...incredibly useful for the efficient generation of individual (and multiple) genetic perturbations in single cells...
Showing: 321 - 340 of 1140 results