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We narrowed to 1,144 results for: Gene

Showing: 321 - 340 of 1144 results

  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is a sucrase... in the chromosome is to disrupt expression of a gene with an antibiotic resistance cassette. This leaves...sometimes interfering with expression of surrounding genes. However, there are ways to create scarless mutations...Red that contained one of eight bacterial toxin genes isolated from E. coli or Psuedomonas aeruginosa....toxins, and phage holins. They hypothesized that genes that had evolved specifically for toxicity would... pathogenic Proteobacteria. We placed the toxin genes under control of a rhamnose-inducible promoter, .... "Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004...

  2. Evolution of Lab Techniques

    Type
    Blog Post
    ...particular gene mutation, southern blotting can be used to identify the presence of that mutated gene. In southern...the location of genes. With this knowledge, researchers were able to target specific genes for deletion ...southern blotting being used to identify a gene mutation is the genetic test used for sickle cell anemia (2)...probe (for example, has the sequence of the mutated gene the researcher is searching for), then the probe...to identify the presence of the mutated β globin gene that causes sickle cell anemia. The mutation happens...questions. Real-time PCR detects and quantifies a gene or DNA sequence of interest by recording DNA abundance...fluorescence in situ hybridization for detection of gene copy number changes." Protocols for Nucleic Acid...

  3. Hot Plasmids - October 2020

    Type
    Blog Post
    ... insert the FP gene into the target gene’s native locus, but introducing large genetic changes in C. elegans...glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library at Addgene CasRx ...cassette exons and disrupts genes with alternative exons. Get the library from Addgene New from the viral...library inhibits over 18,000 human genes. Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed...So how can you use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet... validated this tool by targeting six different genes whose protein products have distinct localizations... number, generally very small, and can contain different neurotransmitters. Using genetically encoded ...

  4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...investigation into the regulation of your favorite gene. But have no fear - I’m here to introduce you to...genome – could be affected, leading to unpredicted gene expression changes (Sapozhnikov and Szyf 2021, Charlton...interact with transcriptional machinery to modify local gene expression, confounding any potential conclusions...causal relationship between DNA demethylation and gene expression (Sapozhnikov and Szyf 2021).    ... expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck and happy editing...Vogelstein B (1983) Hypomethylation distinguishes genes of some human cancers from their normal counterparts...Methylcytosine Hydroxylation, Active DNA Demethylation, Gene Transcription, and Memory Formation. Neuron 79, ...

  5. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...., 2018). Hybridomas can lose gene expression of the mAb-encoding genes or fail to revive after cryopreservation...monoclonal antibodies that are generated in vitro using synthetic genes that are typically expressed from... integrated sequence in a stable cell line. The genes encode the heavy chain and light chain for the antibody...Figure 2: (Left) The light chain and heavy chain genes of the antibody are encoded in a plasmid. (Right...better and better at identifying and cloning the genes for antibody expression. If starting from a protein...sequences of the antibodies and then synthesize the genes that encode those amino acids (Tran et al., 2016... Antibody? animation Resources on Addgene.org Check out Addgene's antibody plasmid collection ...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...particles. But, exo-AAVs may open new ways of improving gene delivery. They increase the efficiency of transduction...for shielding neutralizing antibodies and robust gene delivery in vivo." Biomaterials 35.26 (2014): 7598...PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells using exosome-associated.... "Microvesicle-associated AAV vector as a novel gene delivery system." Molecular Therapy 20.5 (2012):...PMC3345986. Meliani, Amine, et al. "Enhanced liver gene transfer and evasion of preexisting humoral immunity... "Exosome-associated AAV2 vector mediates robust gene delivery into the murine retina upon intravitreal...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

  7. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...plasmids from this publication at Addgene. Multichromatic control of gene expression in Escherichia coli...optimized by Tabor’s lab, making light-controlled gene expression in E. coli easier and more robust. Synthetic...used to engineer new pathways with sophisticated gene expression controls. Most cell types do not respond... can be coexpressed for sophisticated control of gene expression. An E. coli light-sensitive two-component... was only about 10-fold, precluding the study of gene expression at very high or very low levels. Schmidl...researchers to better fine-tune the level of output gene expression based on the input light intensity. In...the plasmids from this publication at Addgene. A synthetic genetic edge detection program. Tabor JJ, Salis...

  8. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...lines and are not used for general plasmid selection. Instead, reporter genes are often employed to easily... be assayed conveniently. A direct test for your gene of interest may be another method to assess transfection...conjunction with positive selection to ensure your gene gets targeted to a specific location within the ...used selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action...use G418. This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin...Read More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All Plasmids...the genetic traits of the foreign material and will subsequently pass it on to future generations. Stably...

  9. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...Imagine you’ve just discovered that your favorite gene was described in a CRISPR screen publication. You...actual data. Now you’re wondering if your favorite gene is included in other CRISPR screens and whether ...researchers to query all public CRISPR results for any gene of interest. In addition, users interested in a ... thresholds used by the authors to determine the gene hits for each screen are also captured as metadata...results are reported as the original quantitative gene-level scores provided by authors rather than sgRNA-level...available resource developed by the Biological General Repository for Interaction Datasets (BioGRID) in...Twitter at @biogrid.     Additional resources on the Addgene blog Read this blog post about genome-wide screening...

  10. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes are expressed under native...sequence and a selectable gene product for isolation of successful transformants. Addgene distributes several...be interested in attaching epitope tags to their genes of interest, allowing for easy capture and detection...modifications in S. Pombe, including full and partial gene deletion, overexpression (by promoter substitution...few. Have you used these tools in your own lab? Addgene would love to hear from you, our community, about...

  11. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...526) that (1) enables efficient knockout (KO) of genes in immune cells; (2) is not immunogenic, a necessity...for in vivo studies; (3) is compatible with single gene KO and pooled screening approaches; (4) encodes ... optimize conditions for a screen that assessed genes that regulate antigen-specific CD8+ T cell responses...screen, we used the pRDA_526 vector to knock out 30 genes putatively involved in CD8+ T cell responses to ...Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens...10.1084/jem.20230699. Additional resources on the Addgene blog X-CHIME: Context-Dependent Germline Knockout...

  12. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human...Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window... The plasmids used to generate these lines have been deposited here with Addgene for distribution to the...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular ...With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular ... with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...

  13. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...REVeRT, for gene delivery using reconstitution via mRNA trans-splicing. By splitting a large gene into smaller...the introduction of extraneous genetic material to reconstitute the gene of interest, reducing the immunogenicity...hIBA1a, 466 bp) that drives high microglia-specific gene expression when incorporated in an AAV vector (Figure...doi.org/10.1101/2023.12.12.571321.  Revolutionizing Gene Therapy: The REVeRT Dual AAV Vector System By Vaibhav...AAVs, and then splicing their mRNAs, the functional gene product is reconstituted within the target cells...vector-based therapies. REVeRT supports advanced gene expression regulation, enhancing both the mechanisms...trans-splicing dual AAV vectors for (epi)genome editing and gene therapy. Nat Commun., 14(1), 6578. https://doi.org...

  14. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...available at Addgene CRISPR-Cas9 References, Protocols, and more References: Generation of Gene-Modified ...the CRISPR/Cas9 system to precisely edit specific genes in monkeys. "Our study shows that the CRISPR/Cas9...system enables simultaneous disruption of two target genes in one step without producing off-target mutations...Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014...many human diseases are caused by genetic abnormalities, targeted genetic modification in monkeys is invaluable...invaluable for the generation of human disease models." Addgene Outreach Scientist and fellow blogger Caroline...

  15. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...variations in gene expression, they found that for approximately 40% of the identified genes, the genes were not...explanation. Next, they hypothesized that differences in gene expression could explain why some ancestry groups...groups were differentially dependent on certain genes. This didn’t prove to be correct, either. However, ...sequences for sgRNAs that target ancestry-associated genes.   What does the reference genome actually reference...cases, this may lead researchers to deprioritize genes that could be good targets for therapeutic development...cells’ germline genetic variations. They then wanted to identify cancer-associated genetic dependencies ...ancestry which will reduce the likelihood of generating genetic dependency data with an ancestry-based bias...

  16. Optogenetics Plasmid Collection

    Type
    Collection
    ... AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging...Collections Optogenetics Plasmids Optogenetics Plasmid Collection You may also like... Optogenetics Guide AAV...tables, refer to Addgene's Optogenetics Guide . Interested in more news about optogenetics research? Find... AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent Protein Guide Microbial...Find optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate... Plasmids Optical Switch Plasmids Background Optogenetics plasmids encode proteins that are controlled...

  17. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... lists gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that...the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions...gRNA expression vectors available from Addgene. CRISPR...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other... effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence designed...promoters. Do you have gRNAs you'd like to add to the Addgene collection? Click here to start the deposit process...plasmids added to the list. You can also email help@addgene.org for more information and assistance with the ...

  18. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  19. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...regions — on gene expression. The regulatory element is cloned upstream of the luciferase gene, and expression...different wavelengths. Addgene provides empty backbones with the luciferase gene, a variety of expression...luciferase Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was first cloned...luciferase assays have become a gold standard in gene expression analysis to investigate the effect of...literally thousands of reporter constructs with a gene of interest already inserted. In addition, we have...community: luciferases, which have become common genetic reporters and alternatives to fluorescent proteins...and sometimes cofactors) to be provided and are generally dimmer than fluorescent proteins. On the other...

  20. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...on vectors where the gene of interest is inserted into the middle of the lacZ gene, thus disrupting 𝛽-...where only cells that have gained or lost a specific gene survive (ex: antibiotic resistance marker). In a... the blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase which ...scientists have found that when a portion of the gene is deleted, it creates a non-functional 𝛽-galactosidase...mentioned above. In molecular biology, cloning a gene into your plasmid is often a numbers game. By using...troubleshooting Sanger sequencing results. At Addgene, we now use next-generation sequencing during our QC process....pigment. Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome...
Showing: 321 - 340 of 1144 results