We narrowed to 1,016 results for: LEA

...2017). Gam binds the free ends of DSBs, which may lead to cell death rather than NHEJ repair, thus removing...they are expressed well. The Liu lab modified the nuclear localization signals and codon usage BE4 to create...also made an adenine base editor with improved nuclear localization and expression. This base editor was... There are also RNA base editors, which you can learn more about in this blog post: CRISPR 101: RNA Editing...al., 2018 BE4max, ArcBe4max, ABEmax   Improved nuclear localization and expression Gaudelli et al., ...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature, 551(7681), 464–471. https://doi.org/10.1038...base in genomic DNA without double-stranded DNA cleavage. Nature, 533(7603), 420–424. https://doi.org/10.1038...

... sites in a promoter lead to higher transcriptional activation. Multiple nuclear localization signals ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...SunTag plasmids have coveted “blue flames,” it’s clear that the system is broadly applicable across many...eBook Avoid Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org...

...England Biolabs and Labconscious. I was eager to learn about practices I could bring both to Addgene, and...impact as scientists. Many scientists are already leading the cultural change toward responsibility in our...polystyrene will likely end up in a landfill, but at least we can reduce the rate at which that happens and... it offsets the cost of regular waste services. Learn about how to start a glove recycling program at ...on culture. Encourage your workplace to become a leader in sustainability. Sign up for blog posts and...companies, and universities to be sustainability leaders. Take initiative to bring ideas to your workplace...

...exploration. Here, we delve into the importance of leaving room for serendipity during your PhD studies and...often happen when we allow ourselves moments of release and mental freedom. Practical approaches Engaging...not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative solutions...discoveries. So, on your academic journey, remember to leave a little space for the unexpected — it might just...Academia: Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned to industry...Experimenting with New Careers While in Grad School How to Lead a Great Meeting Form Your Own Peer Mentoring Group...

...Flame Awards. What's in a flame?     We’re always pleased when scientists around the world choose to share... serves to congratulate researchers who have at least one plasmid deposited at Addgene that has been distributed...Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute our plasmids via Addgene. Given the... us hours of work responding to requests from colleagues.” In addition to helping labs save time, we’re... plasmids and have not yet received your award, please contact [email protected] to claim your Blue Flame...deposit. Begin yours here. It has been my genuine pleasure to send these small tokens of thanks for the incredible...

...serve them better. Here’s a little bit of what we learned. Check Out Our Microbiology Page! The start of ...improving lives worldwide and the presentation made it clear how important it is for us to improve our resources...science education and outreach ourselves, we can a learn a lot from these ASM pros and hope to leverage their...was also a great place to catch up with former colleagues! I ran into past ASM President Tim Donohue (see...their materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read...CRISPR Methods for Bacterial Genome Engineering Learn how to Network at Scientific Conferences Resources...

...industry.” In his article On Leaving Academe, Terran Lane is asked by his colleagues and friends why he would...in the Boston area that demonstrate that 85% of leaving postdocs pursue a career outside the traditional... like this without sufficient data? Starting to learn about the diverse options and how to take advantage...there. Additional Resources on the Addgene Blog Learn about Possible Science Careers How to Negotiate ...for Scientists Series Resources at Addgene.org Learn About Careers at Addgene ...

...of your colleagues. The young scientists that are reading your peer-review comments will learn from them...delivering constructive critique can help your colleagues at the receiving end, if not for this specific...repositories will ensure that the results of your colleague’s hard work will be maintained and made available...comment differently if I would be speaking to a colleague in the office next door. So be kind, helpful, ...writing a review article Resources on Addgene.org Learn about depositing plasmids pre-publication Find molecular...

...on Precision Genome Editing with Programmable Nucleases. Check out #KSgenome on Twitter if you missed ...the CRISPR field forward. To do that, we need to learn much more about cellular DNA repair pathways. An...An emphasis on cellular repair post-CRISPR cleavage When I first looked at the conference agenda, I was...would open not with an introduction to CRISPR nucleases, but with a session on DNA repair. What stuck ...) Delivery The RNP delivery method consistently leads to high on-target editing and low off-target editing... tool supports over 250 genomes, and they just released a brand new CRISPOR manual to kick off 2018! "...

...out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group We don’t all...matter why, now is a great time to find a few colleagues to work together on career and life goals. For...scientist for show wrestling, founded a nonprofit, learned to play the ukelele...perhaps you get the idea....hope you all know by now that people get jobs at least 85% of the time because of relationships. It’s much...conversations. If you set yourself a goal of doing at least two informational interviews a month you will make...academia and not in academia is run by grant funding. Learning to write grants quickly, efficiently and effectively...

...of scientists from MIT tapped into this data to learn about trends in synthetic biology and DNA synthesis...database. Through a series of simulations and machine learning, they created a classifier that predicts whether...gene and a synthetic version of that gene was a clear predictor of whether the gene is synthetic or natural...applications in monitoring the accidental or intentional release of engineered organisms. “Is [the organism] engineered...Nielsen, Alec AK, and Christopher A. Voigt. "Deep learning to predict the lab-of-origin of engineered DNA...Addgene blog Read blog posts about synthetic biology Learn more about codon usage biases Resources on Addgene.org...

...start – it is the discussion after that will lead to learning. Listen to our podcast interview with Harvard... take informal minutes on what was covered and learned, especially noting action items that are identified...Harvard Medical School researcher Connie Cepko to learn about her mentoring style. Set goals with a mentor.... There are many advantages to being a Mentor: Learn other perspectives Develop coaching skills Encourage...Encourage your creative thinking Enhance leadership skills Support inclusion, diversity, and open communication...

...drug discovery, and synthetic biology. Read on to learn more about the five awardees and their promising...their use as food, infusions of L. taraxacifolia leaves are widely used to treat several diseases. Her ..., the most common type of stroke and the third leading cause of death and disability worldwide. An ischemic...ischemic stroke occurs when arteries leading to the brain become blocked, resulting in reduced blood flow...awards. Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant...Expression Systems Synthetic Biology Resources Learn about Global Plasmid Distribution ...

...game that would force my students to use concepts learned from previous lectures, articles and books. A game...board with them. My impression was that they had clearer ideas about gene therapy in the following lessons...perhaps the ideas spurred by this “gamification” will lead to new cures for disease someday! Accessing and ...modify BUILD YOUR PLASMID or generate games like it, please freely share your projects with us! This exchange...want to receive our modifiable powerpoint file, please feel free to contact us at: [email protected] tools for teaching developmental biology Learn how BioBuilder can help teach students about Synthetic...

...back: David Gordon, Jeff Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is to discover key human...FDA-approved). This is a big job, but under the leadership of Nevan Krogan at UCSF we’ve brought together...preclinical studies already. We are following up on these leads as we speak, with collaborators in Paris and New...affected in various ways by the pandemic, it is clear that we have to stick together (6 feet apart), to...shout out to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou, Helene Foussard, and Dave Gordon)... plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with your order...

...while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After realizing...safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind Gram’s crystal ...drop by drop onto the smear until the alcohol runs clear and immediately rinse. Flood the smear with the ...Rinse the slide in tap water until the runoff is clear, blot gently to dry, and then view under an oil ...peptidoglycan membrane is unable to retain the stain, leading to its decolorization. In contrast, counterstain...visualizing differentiations between bacteria, leading to safer medicine and more successful experiments...

...Avoid star activity. Some endonucleases (for example BamHI) are capable of cleaving sequences which are similar...cloned the plasmid yourself or obtained it from a colleague down the hall, it is always a good idea to take...incubating your DNA with restriction enzymes which cleave the DNA molecules at specific sites and 2) running...manufacturers make High Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid...is not just necessary but also an easy process. Please vist Addgene's resource for plasmid verification...

...review see Maday et al., 2014). It is currently unclear which proteins facilitate transport of neuronal...expansion of RABV. This improvement resulted in a clearer picture of neuronal connectivity in the mammalian... mapping of the neuronal circuitry involved in learning and memory (Kohara et al., 2014; plasmids at Addgene...particle is taken up on one side of the cell and released again at another side of the cell after intracellular...transport without actually causing infection or leading to transgene expression within the first cell. ...development of more powerful AAV serotypes, and perhaps lead to controlled trans-synaptic spread of AAV vectors...21782903. McFarland, N.R., Lee, J.S., Hyman, B.T., and McLean, P.J. (2009). Comparison of transduction efficiency...

...affected by light. Long-term light exposure can also lead to cellular phototoxicity, and experimental success...can be affected by both autofluorescence and photobleaching. Researchers have long been interested in using...reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University have developed Nano-lantern... cells. As Nano-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple...applications. For long-term imaging, especially where photobleaching and phototoxicity are a concern, these constructs...Addgene. Additional Resources on the Addgene Blog Learn about Photoactivatable Fluorescent Proteins Check...

...Blog Learn About our Cancer Colleciton Pages Use the ClonTracer Library for Lineage Tracing Learn About...to well-annotated cellular pathways). It wasn’t clear whether gene-agnostic bioassays could accurately...proud to have worked closely with our Addgene colleagues to make these available to the scientific community...10.1016/j.coisb.2017.02.002. We hope that by releasing these new and exciting plasmid collections (together...clinical sequencing studies. At the Broad, my colleague Cory Johannessen and his team have recently demonstrated...