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...lends itself well to assays like western blots and ELISAs. Chromogenic reactions produce a color change, ...immunohistochemistry, though they are also a common choice for ELISAs.  Biotin Biotin is used in a range of antibody-...BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking about these...

...CytERM with mTurquoise2 and dTomato, which are established monomeric and dimerizing FPs respectively, are...compared with mEGFP or mTurquoise2, which are established true monomeric fluorescent proteins. Concluding... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

...producing the fluorescent protein of interest under realistic conditions, i.e. with low excitation power. By...power experiments. Measuring photostability at realistic powers will provide better insight into the photostability...photostability in living cells provides a much more realistic view of photostability. Selecting a photostable...

...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization...optogenetics experiments in specific cell populations. Listen to Our Podcast Interview with Connie Cepko Combining...

...contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog and is part...you will likely have is sifting through the long list of titles for something that is actually useful ...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...

...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus...Travers M, Chen Y, Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy...

...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...in less than a couple of minutes. How does one accomplish all of that? What follows are some tips to help...logical progression of your work, and you can accomplish this by employing a neat and organized format...

...has developed a new, plasmid-based biosensor to visualise changes in histone methylation in living cells...substrate (the histone-derived peptide) which can localise the construct specifically to the histone protein...in the middle of a FRET pair, which is used to visualise the results. When methylation occurs at the histone-derived...

...more likely to view your proposals favorably. 5) Listen to your supervisor’s viewpoint and brainstorm about...with your supervisor, prioritize your action item list and set some approximate timelines. Be sure that...conflicts if you communicate your ideas assertively, listen to his or her viewpoint, and discuss options for...

... advantages and disadvantages. Here is a table listing some of them: Characteristic ESC ASC Self-... For many years, ESCs or ASCs were the only realistic options for scientists working in regenerative... pursue a career in science communication and publishing after completing her postdoctoral training from...

... for you and your goals. Making a List of Schools Creating a list of schools is one of the first steps.... With virtual options, you can narrow down your list of schools by meeting with professors - a/k/a Principle...

...as dot blot, enzyme-linked immunosorbent assay (ELISA) (Grimm et al., 1999), quantitative PCR (qPCR) (...blog post.  We generally do not recommend using ELISA-based titering assays, since those will quantify...Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production of recombinant...

...also been a great place to reach out to local establishments such as museums, professional organizations... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

...techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your...to consult the literature to see if anyone has published a protocol that would work for your system. Addgene... are a few plasmids at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter...

...cistron will start with the Proline.  The table below lists the four common 2A peptides employed by scientists...to express two or more genes, some of which are listed below. We should note that these vectors are primarily...gene or genes of interest. Some of the plasmids listed in the table above (and their related plasmids)...

.... What the BioBricks Foundation is seeking to accomplish is to make the engineering of biology much easier...like this conversation we are having now is in English, and then there are languages to communicate with.... The question is who owns languages? Who owns English? Well, everybody does; it's free to use and that...

...some accountants running our math tables!  Be realistic about what you can teach  5-10 year olds in a ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated...them had imagined for a scientist. I think that establishing this history helped demonstrate that a) I was...

...Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell, and Amy Springer), this group now connects...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

...This post was originally published on the Quilt Genomics Blog and is republished here with permission. Quilt...identified 23-mer gRNA. Off-target determination was established with Bowtie2 using the parameters first described...

... All pCXLE vectors listed below in Table 1 are available at Addgene. Table 1: List of pCXLE episomal plasmids...removal of the residual viral particles and establishment of virus-free hiPSCs usually require about 5...