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We narrowed to 564 results for: PAC;

Showing: 321 - 340 of 564 results
  1. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...well-documented history of racial bias, which still impacts and influences current research. Understanding ... reflects European ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency...
  2. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...much the spread of the virus to adjacent areas impacted the experiments we wanted to perform. It required...injection titers (1,2), and cell types used for packaging viral particles (6), can influence vector performance...
  3. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...event-planning base and will know how to reserve space, time events, and network for volunteers. Speaking...curriculum, would be hard-pressed to maintain the pace of CRISPR. Two of the examples I used in my presentation...
  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...
  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...decided to take a different approach. His own most impactful learning experiences happened in the lab, and ...analyze their recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function...
  6. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Cas9’s, but with the direct repeat preceding the spacer rather than following it. The Cpf1 crRNA is also...counterparts, they will also be easier to deliver in low-capacity vectors, such as adeno-associated viral (AAV) ...
  7. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...application and submitting it, to evaluating the capacity of an organization to receive and manage funds...up-to-date on the changing guidelines and regulations impacting grant management, we track grant opportunity deadlines...
  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...from quenching by aqueous solvent. This tightly-packed structure explains the importance of the entire...stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be used, when...
  9. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only two 24 nt oligos... proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of the crRNA can be customized as well, including...
  10. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...sharing knowledge allows for life to happen without impacting the overall work. Each of our sprints is themed...
  11. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...colleague and give you a million dollar startup package. There are three steps to chalk talk preparation...have developed your research vision, you need to package it as a roughly 30 minute chalk talk. You will ...
  12. How to Be an Excellent Trainee

    Type
    Blog Post
    ... walking through the protocol, by going to each space in order to double-check you remember where the ...Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition Mentoring for Scientists...
  13. Antibody Guide

    Type
    Collection
    ...antibodies are collected, tested against the antigen, packaged, and sold. This is the most cost-efficient way...antibodies to upwards of twenty. Laser and software capacity theoretically allow for roughly fifty different...
  14. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution...
  15. Five Popular Model Organisms

    Type
    Blog Post
    ...mice to study how different mutations in leukemia impact different treatment regimens (Zuber et al., 2009... our own cells. Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes ...
Showing: 321 - 340 of 564 results