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  1. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...application and submitting it, to evaluating the capacity of an organization to receive and manage funds...up-to-date on the changing guidelines and regulations impacting grant management, we track grant opportunity deadlines...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...from quenching by aqueous solvent. This tightly-packed structure explains the importance of the entire...stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be used, when...

  3. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only two 24 nt oligos... proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of the crRNA can be customized as well, including...

  5. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...sharing knowledge allows for life to happen without impacting the overall work. Each of our sprints is themed...

  7. How to Be an Excellent Trainee

    Type
    Blog Post
    ... walking through the protocol, by going to each space in order to double-check you remember where the ...Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition Mentoring for Scientists...

  9. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...colleague and give you a million dollar startup package. There are three steps to chalk talk preparation...have developed your research vision, you need to package it as a roughly 30 minute chalk talk. You will ...

  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution...

  11. Five Popular Model Organisms

    Type
    Blog Post
    ...mice to study how different mutations in leukemia impact different treatment regimens (Zuber et al., 2009... our own cells. Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes ...

  13. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...of interest that are found in the extracellular space. The targeted epitopes can be motifs within transmembrane...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...

  14. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. ...

  15. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...sample migrate through the stacking gel at the same pace and enter the resolving gel at the same time where...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9.../doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J,...

  17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing...particularly large protein, and this fusion allows for packaging into viral vectors and targeted delivery into ...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection of host cells....

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...
Showing: 321 - 340 of 555 results