We narrowed to 665 results for: SIM;

...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...

...getting too nervous or having a memory lapse, I simply pause and take a deep breath before the panic sets...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes...

...been clinically exploited for antiviral purposes similar to that of vaccines. If you know the sequence of...responses can mount in the absence of infection. Similarly, if endogenously synthesized dsRNA finds its way...

...inhibition, with TRIM-away targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21...control will enable future researchers to better simulate physiological gene expression regimes that cannot...

...Fandino, L. B., Labasky, M. E., Forostyan, T., Colosimo, L. K., Graham, T. E. (2015). Rapid parallel measurements...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

...With these improvements, labeling efficiency was similar to that of a comparable FISH method. Importantly...By labeling two genes of a given chromosome simultaneously, they could also monitor the spatial relationship...

...to study and engineer them, but what if we had a simple tool that allowed us to rapidly decode the complexity...experiments. CombiGEM-CRISPR: The marriage of two simple platforms CRISPR has revolutionized how we decode...

...protein fusions, and multiple cloning sites. In the simplest case we have a standard backbone with one insert...reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing...

...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...

...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability... albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used...

...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

...and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as it applies to ...

...task, action, and results. While this method seems simple, I found it hard to remember to describe all four...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...

...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice... from each other. In a direct approach, that is simple - but what about when you need to use an indirect...

...cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...

...therapy currently in clinical trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two ...therapy, since TTR is primarily made in the liver. Similarly, for cardiovascular disease approaches targeting...

... the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant antibody...that may not work well in animals because of similarities to host proteins. After the antibody of interest...

...and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order to assess the effect of removing...phenotype. Again ABE8.20-m was the winner. It could simultaneously edit 3 genes (98.1%, 98.3%, or 98.6% efficiency...