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  1. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering...different antigen; binding of each can be either simultaneous or independent of the other (Shahied, 2004)....

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

  3. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...

  5. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...protein fusions, and multiple cloning sites. In the simplest case we have a standard backbone with one insert...reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing...

  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...plasmid deposit process can be broken down into four simple steps: Send plasmid information to Addgene Material...the Deposit Spreadsheet is the quickest option. Simply download the spreadsheet and copy and paste your...

  9. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    ... For some transgenes, transfection optimization simply is not enough to get a sufficient titer; this is...volumes of lentivirus routinely, you may opt for simply increasing the number of 10 cm2 dishes. While this...

  13. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

  15. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...cutting most anywhere within the RNA will do. Similarly, if you want to knock out a gene, a frameshift...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...

  16. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...task, action, and results. While this method seems simple, I found it hard to remember to describe all four...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...

  18. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

  19. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice... from each other. In a direct approach, that is simple - but what about when you need to use an indirect...
Showing: 321 - 340 of 651 results