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We narrowed to 661 results for: SIM;

Showing: 321 - 340 of 661 results

  1. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

  2. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...

  3. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...getting too nervous or having a memory lapse, I simply pause and take a deep breath before the panic sets...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    ...been clinically exploited for antiviral purposes similar to that of vaccines. If you know the sequence of...responses can mount in the absence of infection. Similarly, if endogenously synthesized dsRNA finds its way...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...protein fusions, and multiple cloning sites. In the simplest case we have a standard backbone with one insert...reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing...

  7. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...

  8. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... For some transgenes, transfection optimization simply is not enough to get a sufficient titer; this is...volumes of lentivirus routinely, you may opt for simply increasing the number of 10 cm2 dishes. While this...

  12. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

  16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...plasmid deposit process can be broken down into four simple steps: Send plasmid information to Addgene Material...the Deposit Spreadsheet is the quickest option. Simply download the spreadsheet and copy and paste your...

  17. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...task, action, and results. While this method seems simple, I found it hard to remember to describe all four...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...

  19. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

  20. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice... from each other. In a direct approach, that is simple - but what about when you need to use an indirect...
Showing: 321 - 340 of 661 results