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We narrowed to 732 results for: SON

Showing: 321 - 340 of 732 results

  1. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...trans-splicing or overlapping vectors: a quantitative comparison . Molecular Therapy, 4 (4), 383–391. https://...

  2. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...different projects, you might not be able to make any reasonable progress on any one of them. That’s why, as I...recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do lists. Because...Bored and Brilliant” for learning more fantastic reasons to remove yourself from distractions every once...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be damaged at the air-water...  Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M....Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide...

  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ... a fluorophore (Figure 1).   Figure 1: Comparison between how direct immunofluorescence and indirect... will focus on this technique here. One of the reasons that indirect IF is more common is that secondary...primary, resulting in signal amplification. Another reason is cost effectiveness. Generating and validating...

  5. Evolution of Lab Techniques

    Type
    Blog Post
    ...be quantified by the strength of the signal in comparison to a control sample. Southern blotting applications... sequencing of many samples at once for later comparison. These next-generation sequencing techniques ...Sanger, Frederick, Steven Nicklen, and Alan R. Coulson. "DNA sequencing with chain-terminating inhibitors...

  6. Finding Your Perfect Job After University

    Type
    Blog Post
    ...being mentally challenged. For these and other reasons, I started  looking for other career options after...government loans to study a MSc, so I had to take out a personal bank loan. I saved money from my role in Industry...or another country of the Commonwealth. Another reason why I chose Australia was because it is an English...

  7. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...cross country boundaries for both professional and personal development, is essential to excelling in the ...adapt their mentoring styles to the mentee’s personality and professional needs at any time. In its Pilot...advisor. For their own part, mentors receive immense personal gratification as they witness their student’s ...

  8. Important Considerations When Using AAVs

    Type
    Blog Post
    ... were suitable for our needs for the following reasons: Safety: Since AAVs are nonpathogenic, we are...Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics...applications, we may also be able to use these comparisons to enhance our understanding of receptor physiology...

  9. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...better plan your transductions and use a range of reasonable MOIs. 2. Create a monoclonal Cas9-expression ... gRNA plasmids on our website. There are a few reasons to target different regions of the gene with multiple...testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months to knockout a...

  10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...that contains sequence complementary to the dsRNA (Wilson and Doudna, 2013). RNAi consists of two non-exclusive..., Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential...:dev186452 . https://doi.org/10.1242/dev.186452 Wilson RC, Doudna JA (2013) Molecular Mechanisms of RNA...

  11. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...difficult to trace. These include the laboratory personnel, the serum, the cell culture media, water baths...your cell cultures because you are not the first person to face this kind of problem. Educate others when...Sung H, Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species...

  12. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...commonly used for recombineering can be found in Thomason et al and Sharan et al (a few strains can also...ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering: A ...19180090. PubMed Central PMCID: PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N...

  13. Typing CRISPR Systems

    Type
    Blog Post
    ...P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution...https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, ... Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning diverse Type IV structures...

  14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction... excited, it can transfer energy directly via resonance to the second fluorophore (the acceptor) if the...PMID: 17512008. 4. Fessenden, James D. "Förster resonance energy transfer measurements of ryanodine receptor...

  15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...heteroduplex transitions back to DNA duplex, and it’s the reason guide sequences have a maximum length of 20 bases...Sternberg et al., 2015).     Figure 8: Comparison of Cas9:RNA crystal structure (left, PDB ID 4ZT0..., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis...

  16. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...for cloning but PX330 only uses 20 nt? A16: The reason for the 30 nt region on the upper is because the...doi.org/10.1126/science.1231143  Elliott, B., Richardson, C., Winderbaum, J., Nickoloff, J. A., & Jasin...cells differing exclusively at two early onset Parkinson Point mutations. Cell, 146(2), 318–331. https:...

  18. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ... research, for decades. Its short life cycle, reasonably close genetic homology to humans, and small size... Rachel Leeson...

  19. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...repositorio. Este increíble regalo permitirá que más personas accedan a recursos útiles para sus experimentos... Rachel Leeson...
Showing: 321 - 340 of 732 results