We narrowed to 421 results for: SUA

1. Antibodies 101: Flow Compensation

   Type

   Blog Post

   ...between machines or the program you’re using, but usually you can click on the correct box and either manually...

2. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   ...fluorescent molecules can be quite broad, detectors will usually only pick up a small portion of that spectra to...

3. Of Myc and Men

   Type

   Blog Post

   ...endoplasmic reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ...enough plasmid to complete my experiment. AAV is usually the limiting reagent for my experiments, so I always...

5. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   ...temperature cycling. The final PCR product is often visualized on a diagnostic agarose gel to confirm product...

6. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...and more AAV1 versions will be released soon. Visualize axonal projections and putative pre-synaptic terminals...

7. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   ... After the ELISA step, the narrowed-down pool (usually 10-30) of sybody candidates are sequenced for identification...

8. PCR Applications for Copy Number Variation Assays

   Type

   Blog Post

   ...sequence present in the same amount in all samples (usually to a ubiquitously expressed, one-copy ‘housekeeping...

9. The Scientific Conference Poster Session: Tips for Success

   Type

   Blog Post

   ...engaging with you? Be sure to use your poster as a visual aid, not a crutch. Even the most prepared of us...

10. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    ...the session, if someone comes up to my poster, I usually greet them and say something like “Let me know ...

11. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    ...infection rates in your experiment. Figure 2: Visual representation of common AAV serotypes used for...

12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    ...happen in a matter of minutes. Cost: Funding is usually limited - what cloning approach should you use ...

13. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    ...labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent protein...

14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    ...predominantly fluoresce a second color. This change is usually hypothesized to be a result of chromophore oxidation...

15. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    ...gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre/Lox recombination...

16. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    ...difficult to target therapeutically due to their unusually “smooth” shape and lack of “druggable” domains...

17. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    ...proteins. One of the major applications of FPs is to visualize the localization, dynamics, and behavior of a ...

18. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    ...error-prone non-homologous end joining (NHEJ), usually results in a loss-of-function mutation within the...

19. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ...tissues transparent are enabling microscopes to visualize the cellular architecture of whole tissues in ...

20. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    ...below. IRES Elements Translation in eukaryotes usually begins at the 5’ cap so that only a single translation...