We narrowed to 424 results for: SUA

1. Gaining Leadership Skills Volunteering at a Professional Organization

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   Blog Post

   ...Volunteering on these committees was real work; meetings usually resulted in actionable tasks and I made a point...

2. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   Blog Post

   ...difficult people: Hostile-aggressives, who are usually antagonistic and impolite, Complainers, who spend...

3. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   ...fluorescent molecules can be quite broad, detectors will usually only pick up a small portion of that spectra to...

4. Of Myc and Men

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   ...endoplasmic reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro...

5. Addgene's Cancer Collection Pages Connect the Research Community

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   ...difficult to target therapeutically due to their unusually “smooth” shape and lack of “druggable” domains...

6. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...proteins. One of the major applications of FPs is to visualize the localization, dynamics, and behavior of a ...

7. 3D Printing Meets CRISPR Cas9

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   ...Learn All about CRISPR Use CRISPR for Genome Visualization Tips for a First Time CRISPR User by a First...

8. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent protein...

9. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...predominantly fluoresce a second color. This change is usually hypothesized to be a result of chromophore oxidation...

10. The AAVantages of AAV in CRISPR Screens

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    ...infection rates in your experiment. Figure 2: Visual representation of common AAV serotypes used for...

11. Production of Virus in Insect Versus Mammalian Cells

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    ...production when roughly 60–70% of cells have died, usually after four to five days. This means that viral ...

12. PCR Applications for Copy Number Variation Assays

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    ...sequence present in the same amount in all samples (usually to a ubiquitously expressed, one-copy ‘housekeeping...

13. Polymerase Chain Reaction Overview and Applications

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    ...temperature cycling. The final PCR product is often visualized on a diagnostic agarose gel to confirm product...

14. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...and more AAV1 versions will be released soon. Visualize axonal projections and putative pre-synaptic terminals...

15. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ...enough plasmid to complete my experiment. AAV is usually the limiting reagent for my experiments, so I always...

16. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...happen in a matter of minutes. Cost: Funding is usually limited - what cloning approach should you use ...

17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ... After the ELISA step, the narrowed-down pool (usually 10-30) of sybody candidates are sequenced for identification...

18. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...engaging with you? Be sure to use your poster as a visual aid, not a crutch. Even the most prepared of us...

19. Tips to Make the Most of a Scientific Conference

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    Blog Post

    ...the session, if someone comes up to my poster, I usually greet them and say something like “Let me know ...

20. Antibodies 101: Flow Compensation

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    Blog Post

    ...between machines or the program you’re using, but usually you can click on the correct box and either manually...