We narrowed to 365 results for: Scrib

...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...

...probes are based on cofactor binding proteins as described above. The smallest tags are peptides that are...

...combining the AAV and CRISPR-based approaches is described in several recent reviews (Howes and Schofield...

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

... through Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits...

...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...

...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...

...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...

... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...

...In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin...

...between the targets. Or, it might be sufficient to describe or classify the labeled structures visually; for...

...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...

...the opsin into the correct orientation to be transcribed (Figure 2) (Sharma and Zhu, 2014). How does FLEx...

...available through Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of...

... on a reverse transcriptase enzyme to reverse transcribe back into DNA before being inserted into new ...

... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...

...BAC DNA can be modified with any of the below described approaches.    Bacterial strain with integrated...

...are designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila...

...sequences, and more. Output_Compact: A text file that describes which genes were included, and how many gRNAs ...

... tested and will help healthcare professional prescribe and identify potential beneficiaries. Collaborative...