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Showing: 321 - 340 of 618 results

  2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ... a small subset of cells? Other proteins may also be utliized as scaffolds, including the set of red fluorescent...recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks transcription...absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre isn’...

  4. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...ready for restriction digestion. Digest Your DNA Set up restriction digests for your PCR product and recipient...recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance, a...

  5. CRISPR in the Clinic

    Type
    Blog Post
    ...especially for systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies...

  8. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...profiles have limited their usability in clinical settings. On the other hand, vectors derived from AAV have...the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until...

  9. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...case, you design your targeting vector such that a set of three LoxP sites flank the resistance gene and...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron.    Figure...

  10. 6 Tips for Grant Writing

    Type
    Blog Post
    ...Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you can ...

  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...of several lasers simultaneously, can be used to set the laser output without manual manipulation of the...will open but will not be given an opportunity to “reset” and become receptive to light once again. Therefore...

  14. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...heterogeneous population of mutant cells, with each cell or set of cells containing a mutation in a different gene...

  17. Protocol - How to Streak a Plate

    Type
    Protocol
    ...and your initials. Labeling within a laboratory setting is important for organization, and it is recommended...

  18. Of Myc and Men

    Type
    Blog Post
    ... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...
Showing: 321 - 340 of 618 results