We narrowed to 617 results for: Set
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TypeBlog Post...for this transition. Second, a coach will help you set goals and then hold you accountable for carrying ...
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Plasmid Cloning by PCR (with Protocols)
TypeProtocol...ready for restriction digestion. Digest Your DNA Set up restriction digests for your PCR product and recipient...recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance, a... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...profiles have limited their usability in clinical settings. On the other hand, vectors derived from AAV have...the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...case, you design your targeting vector such that a set of three LoxP sites flank the resistance gene and...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron. Figure... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...happens when the antibody is produced from a new set of animals), and wrong application (different experiments... -
CRISPR in the Clinic
TypeBlog Post...especially for systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...sample, we’ll then load it on to our new MiSeq and set up the run making sure to note the appropriate barcode... -
6 Tips for Grant Writing
TypeBlog Post...Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you can ... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...recommended pay scale – used by many institutions to set postdoc wages. The second event of note is a ruling...Assistant Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...read-through. Chris Voigt’s lab has characterized a set of prokaryotic terminators and deposited several ... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...of several lasers simultaneously, can be used to set the laser output without manual manipulation of the...will open but will not be given an opportunity to “reset” and become receptive to light once again. Therefore... -
Genome-wide Screening Using CRISPR
TypeBlog Post...heterogeneous population of mutant cells, with each cell or set of cells containing a mutation in a different gene... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...supervisor, prioritize your action item list and set some approximate timelines. Be sure that you keep... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post...steps in a protocol as you finish them, turn on pre-set timers for incubation steps, and save the entire ... -
Protocol - How to Streak a Plate
TypeProtocol...and your initials. Labeling within a laboratory setting is important for organization, and it is recommended... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...a large number of hits to one sequence/gene - we set our threshold at >100 hits - immediately raises a... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...containing serum in each well. A 200 ul pipettor is set to 200 ul, wiped down with 70% ethanol, and a box... -
10 Basic tips for mammalian cell culture
TypeBlog Post... you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy about splitting... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...list of posters of interest, then you’ve already set yourself up for success. But sometimes, attendees... -
Of Myc and Men
TypeBlog Post... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...