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  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

  4. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...reels in the non-target strand of DNA much like a fisherman would reel in a big catch. It was originally suspected... Alyssa Shepard...

  6. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... genome that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin... Lalanne, J.-B., Nathans, J. F., Lee, D. S., & Shendure, J. (2024). Chromatin context-dependent regulation...

  9. Of Myc and Men

    Type
    Blog Post
    ...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

  11. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...temperature for mammalian cells and one which diminishes the endonuclease activity of the enzyme. In vivo...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

  12. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...-80°C. Subsequent freeze and thaw cycles reduce shelf life. To recover bacteria from your glycerol stock...it from thawing completely and will improve the shelf life. It is very important that you shake the glycerol...

  14. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

  16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...structure and could also cause your target to be washed away. Furthermore, these chemicals can also alter...: Always always always look at your antibody datasheet to see if it has been tested in IF and if a protocol...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...wired into circuits through synaptic connections (Shepherd, 2004). Understanding the structure and function...25915120. PubMed Central PMCID: PMC4573404. 2. Shepherd, G.M. (2004). In "The synaptic organization of...
Showing: 321 - 340 of 595 results