We narrowed to 731 results for: Sis;

...that a plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...scientists can incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters...

...pTargetF system Our original pCas/pTargetF system consists of one plasmid that expresses the S. pyogenes ...engineering case study of N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies... modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica...

...singletons (reads that only appear once) in our analysis. Many pipelines toss these singletons out, and...stranger - Finding mentors for the meeting Just like analysis pipelines for DNA that group similar sequences...variability was organic, as some people need less assistance than others, but we did have some participants...

...of its parts. Elements such as the antibiotic resistance gene and the origin of replication are necessary...elongation factor 1 alpha Constitutive  Tends to give consistent expression regardless of cell type or physiology...expression, but may vary by cell type. Tends to resist promoter down regulation due to methylation or ...

...contributed by guest blogger Joachim Goedart, an assistant professor at the Section of Molecular Cytology...conditions needs to be examined on a case-by-case basis. GFP is sensitive to acid The chromophore of GFP...blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...promiscuous biotin ligase (Kim et al 2016). BioID2 consists of a humanized biotin ligase from A. aeolicus,... assemble when in close proximity. Split-BioID consists of a split BirA* molecule that can reassemble ...compared to the standard E. coli BirA* mutant. RapID consists of this mutant BirA* and an RNA component that...

...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ... IV, were then identified through microfluidic-assisted in vitro compartmentalisation for their high binding...PMC3852148. Warner, Katherine Deigan, et al. "Structural basis for activity of highly efficient RNA mimics of green...

...ability to reassemble fragmented genomes is the basis of several split vector approaches developed in ...transgene expression from split vectors has been inconsistent. A greater understanding of basic AAV biology...vector strategies and lead to greater and more consistent transgene expression. References: Allocca M...

... social science to an audience that I thought consisted of all biologists. The social scientists in the...to them with an answer, and do so if you can. 9. Resist the urge to say everything I allow 1 slide for ...topic or story is all you need, if you do it well.  Resist the urge to show all or even a lot of data.  I ...

...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized... formation: the last puzzle of aminoacyl‐tRNA synthesis." FEBS letters 462.3 (1999): 302-306. PubMed PMID...Sauerwald, Anselm, et al. "RNA-dependent cysteine biosynthesis in archaea." Science 307.5717 (2005): 1969-1972...

...C-terminal HA tag. Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze...supernatant and selected with puromycin. Puromycin-resistant cells were fixed and labeled with primary antibody...the genome (Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc ...

...particularly interesting as I had just completed my thesis on the study of e2Fb, which is a tumour suppressor...but I really struggled with the large scale data analysis, as this is increasingly reliant on programming... to use diagnostic tool for the detection and diagnosis of different types of pancreatic cancer and highlighting...

...as a control. DNA was analyzed by PAGE. The basis of the Surveyor assay is that Surveyor nuclease ...edit. Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence...Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the bands on your...

...coupled with iterative rounds of selection and mutagenesis to refine antibody performance. If you are interested...L. (2019). Antibody Structure and Function: the basis for Engineering therapeutics. Antibodies, 8(4), ...Wilson, D. S., & Keefe, A. D. (2000). Random mutagenesis by PCR. Current Protocols in Molecular Biology...

...plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming...reagent preparation in the lab. Figure 3: csRNP-assisted epitope tagging is scalable to 96-well format...., Gogolok S et al. 2017. Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic...

...depends on it. Deanna McLafferty is an editorial assistant and activist. Washington, DC, USA 38.9072°N 77.0369...scientists – including global warming skeptics – insisted that more data and research funding are critical...trying turn a few exoplanets into a successful PhD thesis. We use the same atmospheric physics to understand...

...undesirable side products generated during chemical synthesis of these molecules. To make large amounts of pure...applications, but there are more! Engineering disease resistance through gene stacking, increasing production ...Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1,...

...number. First you generate four unique kanamycin-resistant plasmids, each containing a different gRNA target... all but one of your gRNAs into spectinomycin-resistant plasmids pX330S-2 to pX330S-(last gRNA number).... Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

...engages the prime editor protein. The prime editor consists of a Cas9 nickase fused to a reverse transcriptase... domain for optimization. They developed phage-assisted evolution strategies to evolve more effective ... J., Osborn, M. J., & Liu, D. R. (2023). Phage-assisted evolution and protein engineering yield compact...

...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...guest, Joachim Goεdhart! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...