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We narrowed to 731 results for: Sis;

Showing: 321 - 340 of 731 results

  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...They first created a defective chloramphenicol resistance gene (CamR) by introducing a point mutation (... adenine base editor would restore antibiotic resistance. To find such a protein, they created a mutant...any restriction enzyme (RE) recognition site, RE analysis of PCR product could be used to detect success...

  2. Hot Plasmids - August 2020

    Type
    Blog Post
    ...CRISPR corner New from the viral service Faster catalysis after yeast-based protein evolution By Joanne ...evolution platform to generate TEV protease variants. Catalysis by TEV protease allows a transcription factor ...CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones...

  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...proteins within the bacterial genome. From this analysis, they identified 53 potential candidate genes ...within Cas13a are essential for RNA cleavage, consistent with known roles for HEPN domains in other proteins...located at the 3’ end of the spacer sequence and consists of a single A, U, or C base pair. In bacteria,...

  4. Bioinformatics at Addgene

    Type
    Blog Post
    ...the accompanying sequencing data collection and analysis eventually outpaced our software, even with continuous...singular purpose (ex. adapter trimming, quality score analysis, k-mer counting, or sequence assembly), but the...would handle plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we specified...

  5. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...concentration, ionic strength and pH, affect binding the lysis buffer may need to be adjusted depending on the ...include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and perform the IP ...For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively, the protein can also be eluted...

  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...that a plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...scientists can incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters...

  7. How to Be an Excellent Trainee

    Type
    Blog Post
    ... is the person you report or talk to on a daily basis who will help you get on your feet with techniques...accordingly in case you have questions or need a little assistance at some point during the procedure. Be an active...everything is magically made up by helpful TAs or assistants, and sitting out on the bench waiting for you...

  9. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed ...Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): 512–20. PubMed PMID: 20506501. 6. Logie,...Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron 89(4): 756–69. PubMed ...

  10. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...pTargetF system Our original pCas/pTargetF system consists of one plasmid that expresses the S. pyogenes ...engineering case study of N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies... modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica...

  11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...singletons (reads that only appear once) in our analysis. Many pipelines toss these singletons out, and...stranger - Finding mentors for the meeting Just like analysis pipelines for DNA that group similar sequences...variability was organic, as some people need less assistance than others, but we did have some participants...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...of its parts. Elements such as the antibiotic resistance gene and the origin of replication are necessary...elongation factor 1 alpha Constitutive  Tends to give consistent expression regardless of cell type or physiology...expression, but may vary by cell type. Tends to resist promoter down regulation due to methylation or ...

  13. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ... IV, were then identified through microfluidic-assisted in vitro compartmentalisation for their high binding...PMC3852148. Warner, Katherine Deigan, et al. "Structural basis for activity of highly efficient RNA mimics of green...

  14. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...ability to reassemble fragmented genomes is the basis of several split vector approaches developed in ...transgene expression from split vectors has been inconsistent. A greater understanding of basic AAV biology...vector strategies and lead to greater and more consistent transgene expression. References: Allocca M...

  15. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...contributed by guest blogger Joachim Goedart, an assistant professor at the Section of Molecular Cytology...conditions needs to be examined on a case-by-case basis. GFP is sensitive to acid The chromophore of GFP...blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

  17. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... social science to an audience that I thought consisted of all biologists. The social scientists in the...to them with an answer, and do so if you can. 9. Resist the urge to say everything I allow 1 slide for ...topic or story is all you need, if you do it well.  Resist the urge to show all or even a lot of data.  I ...

  18. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized... formation: the last puzzle of aminoacyl‐tRNA synthesis." FEBS letters 462.3 (1999): 302-306. PubMed PMID...Sauerwald, Anselm, et al. "RNA-dependent cysteine biosynthesis in archaea." Science 307.5717 (2005): 1969-1972...

  19. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...C-terminal HA tag. Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze...supernatant and selected with puromycin. Puromycin-resistant cells were fixed and labeled with primary antibody...the genome (Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc ...

  20. Finding Your Perfect Job After University

    Type
    Blog Post
    ...particularly interesting as I had just completed my thesis on the study of e2Fb, which is a tumour suppressor...but I really struggled with the large scale data analysis, as this is increasingly reliant on programming... to use diagnostic tool for the detection and diagnosis of different types of pancreatic cancer and highlighting...
Showing: 321 - 340 of 731 results