We narrowed to 931 results for: TIM

...the number of genes that can be manipulated at one time. Weissman’s group has made standard protocols available...available and they are working to further optimize those now. They are also developing sgRNAs that will...there is potential there,” he says. "We have to optimize how we can deliver these tools because obviously...mouse, we can’t rely on genetic engineering to optimize humans for CRISPRi and CRISPRa.” Before considering...information about SAM, including a tool for getting optimized guide sequences for activating any human gene,...

...course, real relationships go further and grow over time, but being interested in the same phosphate of your...connections What can you do to get the most from the time and money you are spending on a conference? One ... onsite and eat together because scientists have time to really connect with others whose company they...yourself. Put your phone away! This is a natural time to meet someone since you are both interested in...promised to send a paper, send it. If you had a nice time, send a note to say so. If you read a paper that...

...developmental delays or autism spectrum disorder. It is estimated that CTD is the second cause of male intellectual...psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6 per 10,000 people globally...of the aorta) are two heart defects that are 50 times more prevalent in women with TS. “The goal of our... bicuspid aortic valve at a rate that is 50-100 times higher than women in the general population,” says... the signalosome of different receptors upon stimulation. “To study cellular transduction events, we will...

... research and clinical applications. Most of the time, when someone speaks about an affinity reagent, ...large enough that their bulk can be a barrier at times. Smaller, slimmer affinity reagents can have deeper...have them, you’ll need to either re-develop or re-optimize your assay for an entirely new affinity reagent...reagents you use, one thing is clear: it’s an exciting time to be a scientist looking for that perfect fit!  ...References Grumezescu, A. M. (2018). Drug targeting and stimuli sensitive drug delivery systems. Elsevier Science...

...sets in. Most of the time, that’s enough–but if it’s not, I can always take more time to check my notes!...share what I've learned along the way. Own your time When I was an undergrad, I was assigned a paper ...lesson: Your presentation will be better if you take time when you need it, rather than trying to push through...resources on the Addgene blog Transferable Skills Guide: Time Management Transferable Skills Guide: Conflict Resolution...

...intentional introduction of the Cre recombinase. This is a time-tested system but does require knock-in of the LoxP...genomic site, or randomly integrated (once or many times) into the genome. Make sure to choose an appropriate...Piggybac dCas9 SAM vector! On/off control all the time If your experimental system requires tunable control...turn expression on/off for short or long periods of time. Thus, there is a lot of overlap with the systems...site-specifically knocked in. This can be labor intensive and time consuming. If your native protein doesn’t require...

...signal at the same time point. E. coli’s BirA* was tested at its optimum 18 hour time point, but one minute...Sometimes it feels like DNA and protein get all the attention.There are numerous ways to detect DNA-protein... interactions in events that occur on a short timescale, such as during drug action or cell signaling....

...monitor their abundance and clonal dynamics over time using next generation sequencing (NGS). The library...implantation. Their goal was to figure out how to optimize protocols for in vivo screening with lentiviral...of each barcoded clone can then be monitored over time by sequencing the barcodes in the population (all...tool in their own work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by ...Many thanks to Carrie and Novartis for taking the time to talk with us and for providing this powerful ...

...to appropriately time the injection about two weeks before the behavior test. This time window between ...measuring locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress ...cleaned thoroughly between animals as olfactory stimuli that were left behind from a preceding animal (...repeated staggered injections may be considered. Timing: High expression levels of the viral vector trangenes...

... for every new prime editing application. And optimizing its sequence is important, as prime editing efficiency... with prime editing, you’ll need to design and optimize a pegRNA for your particular edit. These tips ...with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different lengths...2019). Figure 2: Starting lengths for optimizing your pegRNA primer binding site (PBS) and reverse...tool-specific best practices. Here are some tips for optimizing your tool of choice. Nicking sgRNAs PE3 and ...

... a precursor molecule is made, modified (sometimes many times), and isolated afterwards. To engineer these...other sources; second, characterizing a part or optimizing a TU is much easier when you can quickly test...assembly. These pathways can require a lot of optimization - which is why MoClo is so attractive! It allows...delivering them to these organisms via another optimized platform. Gene Stacking As the name implies, gene...

...an enormous amount of wasted money and time, with an estimated $350M/year lost on low quality antibodies...remix the songs, the CDs could get scratched over time, and it was difficult to keep track of growing collections...molecularly defined antibodies that do not change over time, scientists will know exactly what antibody they...lack sequence data, and may genetically drift over time. Antibodies made from animals are limited in supply...

...publication timeline I think we can speak for all the authors on the paper when we say the timeline to develop...complete the project on such a short timescale. Our article ultimately reported the development of a rapid...the American-lineage of the virus. For the first time, we have also demonstrated our technology on active...

...An estimated 320,000 viruses can infect mammals. Even more abundant are the Earth’s estimated 1031 bacteriophages...of the infectivity of your viral stock, save you time, and allow you to standardize your experiments later...References 1. Anthony, Simon J., et al. "A strategy to estimate unknown viral diversity in mammals." MBio 4.5 ...Stability of a recombinant adenoviral vector: optimization of conditions for storage, transport and delivery...

...on the Addgene Blog First time CRISPR user? Check out our tips for 1st time CRISPR users Learn how to ...factors, can increase a gene’s transcription. Sometimes enhancers are located thousands of base pairs ... interactions at one location in the genome at a time (Liu et al., 2017). Recently the Xu lab developed...epigenetic modifications, we can start to build a timeline of events that surround changes in gene expression...

... 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions of organic...response to a ~25 mT magnet. Gray regions indicate times when the magnetic field is on. Image reproduced ...response to a ~10 mT magnet. Gray regions indicate times when the magnetic field is on. Image reproduced ... into a variety of optogenetic tools, they’re optimistic that MagLOV can be similarly adaptable into future...Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification...

... Attempts have been made to optimize their fluorescence, which ultimately led to the engineering of the...been achieved with these sensors, but further optimization is necessary for these to be as effective at.... The pro of this system is the kinetic response time from signal input to readable output, which generally...swaps capsid proteins from different viruses to optimize them for targeting your cell type of interest....

...viewed 1,904,462 times, and the CRISPR 101 eBook has been downloaded over 72,000 times. Our CRISPR blog...2012 to 2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of requests ...Homology Directed Repair has been viewed 219,289 times, while Validating Your Genome Edit has 76,011 views...Addgene repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a...

...been widely used in biological research for a long time, chromoproteins are just now being developed for...identification is a good place to start before taking on more time consuming and expensive methods. While techniques...manipulations start. Anthony Forster’s lab used codon optimization programs to engineer 14 chromoprotein sequences...lab determined the color intensities, maturation times, and fitness cost of expression. They found that... lab developed as an acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy...

... enable biosynthetic pathway engineering and optimization. However, current best practices for assemblies... often cycled between 37°C and 16°C to provide optimal reaction temps for both the restriction enzyme ...lacI/lacZ cassette (designed by NEB for use in optimization of assembly systems) to produce a blue colony...improved Golden Gate performance, a series of optimization experiments for these more complex assemblies...    References 1. Vladimir, Potapov, et al. "Optimization of Golden Gate assembly through application ...