We narrowed to 752 results for: Uty

... GCaMP containing plasmids are available for distribution. If your experiment involves delivering a calcium...types of searches including depositing labs and institutions. For example, search for “Melina Fan” and you...Lab published in eLife. Addgene is currently distributing 94 plasmids expressing validated antibodies ...

...unlimited production, reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested...The beads are then washed, followed by antibody elution. Whatever method you choose, you may not be able...any temperature shifts. Of course, we are also cautious during the aliquoting and storage process to prevent...

...They then performed sixteen rounds of directed evolution across the full ADAR2DD to further optimize cytidine...   Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b...of RNA editing in research and its potential therapeutic uses We are also curious if REPAIR and RESCUE...

... be to fuse an affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses...KRRWKKNFIAVSAANRFKKISSSGAL 4 Affinity and Purification Binding and elution steps use very moderate buffer conditions FLAG...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity...

...; multiple codons encode single amino acids. Evolutionary constraints have molded which codons are used...express well in any organism. So what are the evolutionary constraints that lead to these preferences and...6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation of codon optimality reveals hidden...

... Variation in ori structure allows plasmids to utilize diverse host replication machinery. For instance...Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. viciae... P. C., & Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based expression...

...in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is...critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https...ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat...

...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...collaborators of Omar and Jonathan and experts in CRISPR evolution, were also working on the project. Using computational...possibilities at the bench and potentially in therapeutic applications. The potential of CRISPR has yet...

...specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al, 2020). Because retrovirus is ...disadvantages: Table 1 (Joglekar et al., 2017)  Table 1 (Gutierrez-Guerrero et al., 2020) Table 1 (Cronin et al.,...–222 . https://doi.org/10.1006/mthe.2000.0116 Gutierrez-Guerrero A, Cosset F-L, Verhoeyen E (2020) Lentiviral...

...Briefly, 293T cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 hours...line was obtained directly from Takara, and is routinely tested for mycoplasma contamination using mycoplasma...Cells transduced with our lentiviral vectors are routinely checked for signs of microbial contamination. ...

...by Gaudelli et al. resulted in the successful evolution of an adenosine deaminase that can act on DNA ...for surviving colonies and subsequent rounds of evolution and engineering gave rise to a mutant TadA (TadA...in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018). Additional...

... variety of fields in order to provide useful solutions to complex challenges. As a cross-disciplinary...citation rates to measure the impact of their contribution. It offers a 5-step process and several examples...Social Networking for Scientists Read about the Evolution of Scientific Impact Data Freedom: The Expansion...

...resistance marker from the vector (abR, Figure 1). The resolution step (“double crossover”) which leads to the ... program) (Latynski and Valentovich 2014). We routinely clone the purified amplicons upstream and downstream... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

...fluorescent aptamer, was identified through directed evolution. First, a pool of RNAs that activate the fluorescence...fluorescence of DFHBI were identified using systematic evolution of ligands by exponential enrichment (SELEX). ... by fluorescence-based selection and directed evolution." Journal of the American Chemical Society 136.46...

...be dominant-negative and/or toxic. A potential solution is to include an in-frame degron, or degradation...in a single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the...Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio...

...external forces such as ionizing radiation, chemotherapeutics, and ultraviolet light. In this post, we will...via cleavage in one of two ways - each junction resolution could happen on the crossing strand (horizontally...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted...

...activity of the original, generated using directed evolution (Tei et al., 2023).  PLD can hydrolyze phosphatidylcholine...matter. Plus, for folks interested in directed-evolution methods, the activity-based selection in mammalian...Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian cells. Nature...

...can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach...perform alanine scanning mutagenesis or targeted substitution of key residues), and in SNP analysis (to introduce... bases at the 3’-end interfere with plasmid reconstitution, and consequently Taq polymerases cannot be...

...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! This post was originally published...efficiency in pancreatic beta and alpha cells utilizing various AAV serotypes. Scientific Reports, 15(... AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic...

...and accessibility, more researchers have been utilizing full plasmid sequencing services to verify the...independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar 25;286...E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution of Multimeric Forms of Circular Plasmids and ...