...Streptomyces, a prokaryotic genus capable of producing a variety of natural products. Recently, this group optimized...base-editing efficiency using XMAS-TREE-based methods at various genomic loci in four different categories. ...  Various Addgenies...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates. The development...as other affinity reagents such as single-chain variable fragment (scFvs) and nanobodies. Addgene will ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

...cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1 mutations on cell... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...

...blogger Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent...Joachim Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but...Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages the Cell Biology Microscopy...

...never completely eliminate the risk that a minor variation in experimental conditions will derail a research...and why other publishers and organizations have various different versions of checklists and guidelines...Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary research ...

...researchers have been able to study the effects of various stimuli on global translation, but a visual technique...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...represent powerful tools for studying translation in various cell types and physiological states. References...

...used N- and C-terminal dronpa fusions. With these various designs, Xin has been able to develop photoswitchable...has demonstrated the utility of these FLiPs in a variety of research applications and particularly notes...be able to use similar designs to create a wide variety of enzymes and proteins with inducible activity...

... ITR sequences from different AAV serotypes that vary in their RBE and T-shaped hairpin loop sequences...non-AAV2 ITRs can be used to drive gene expression at varying capacities. AAV vectors containing the AAV2 ITRs...different combinations of ITR and capsid serotypes vary in their gene transfer efficiency. Our practical...

...resistant to detergents and degradation under a variety of conditions. They are composed of 4 proteins ...robust linear actuators that could be used in a variety of applications. For example, you could use them...Silver in the department of Systems Biology at Harvard Medical School. Follow her on Twitter @jessicapolka...

...Institute today, where he helps researchers in a variety of fields develop RNAi screens to answer their ...more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied to work at the ...Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate the...

...reporter, other luciferases have been identified in various species with differing kinetics, substrate requirements...plagiophthalmus), railroad worm (Phrixothrix hirtus), and a variety of marine crustaceans. Many of these luciferases...firefly(Cypridina hilgendorfii) 465 62 Cypridina (Vargulin) luciferin None Yes Sea pansy(Renilla reniformis...

...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...and writer.” Connie Cepko was surprised by the variety of skill sets she needed to acquire as a professor...

...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...throughout the year since hiring opportunities arise at various times. If the timing and interests align for both...

...mammalian expression vectors containing various spike protein variants are also available. Plasmids are continually... Various Addgenies...

...pressure to select for variants of interest, though. A selection can take the form of varied temperature, nutrient...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...

...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...

...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

...degenerate primer set to amplify IgG variable light (VL) and variable heavy (VH) chain sequences. Fusion...thoroughly validated. The creativity in employing a variety of cloning techniques and troubleshooting methodologies...

... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...to reduce the chances of making an error. Try a variety of things, including (and maybe especially) suggestions...

...model, ubi:zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination... that recombine independently and results in a variety of colors depending on the combination of fluorescent... Various Addgenies...