We narrowed to 532 results for: abo.3

... ways we get your plasmids out there Beyond these 3 big pushes, we try to increase your work’s visibility... will be able to easily find the fruits of your labor and won’t have to repeat the modification. This ...re ever interested in writing your own blog post about a plasmid or set of plasmids you’ve deposited with...

... cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows that the yeast...site-specific recombination in Drosophila, read on to learn about new recombinases suitable for this system. Gerald...recombinases from the Rubin lab For more information about limitations and caveats of Cre usage: Vagaries of...

... term sgRNA. Seed sequence: The 8-10 bases on the 3’ end of an RNA guide (either crRNA or sgRNA). Cas9...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...for particular screens of interest.   Figure 3: Each publication featured in BioGRID ORCS has a dedicated...resources on the Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our...on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for...

...community. These popular plasmids are categorized into a 3-level system on our website:   A yellow flame indicates...Flame awardee Sophien Kamoun of the Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute...Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology Resources on Addgene.org...

...selection of pLKO.1 plasmid in mammalian cells. sin 3’LTR 3’ Self-inactivating long terminal repeat. f1 ori...antisense—TTTTTG 3’ Reverse oligo: 5’ AATTCAAAAA—21bp sense—CTCGAG—21bp antisense 3’ For example...Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO... C.1 Recommended materials C.2 Annealing oligos C.3 Digesting pLKO.1 TRC-Cloning Vector C.4 Ligating and...Determining the optimal puromycin concentration F.3 Protocol for lentiviral infection and selection G....Sequence of pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning...long terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids available ...

...Furthermore, Group Leader position applications require 3 letters of reference- the graduate school mentor, ...yourself to and talk with more senior scientists. 3. Work intensely, but intelligently Before you do your...Nature and Science, for example). This blog post is about how to get the most out of your postdoctoral training...your new model?  PFs need to be regularly thinking about manuscripts. The other items will follow. If you...this is a strong indicator that this person cares about your science and your development. Taken together...Researchers face a fundamental conflict. Science is about exploration, discovering new information. There ...many PFs are reluctant to give talks or posters about their research. They dread giving a departmental...

...Fragment Variables (scFvs). Nanobodies Figure 3: Comparison of the Hcab and nanobody. Nanobodies...molecule chaperone Skp. Front Cell Infect Microbiol 3: . https://doi.org/10.3389/fcimb.2013.00072 Additional...is referred to as an antigen. You can learn more about antibodies in our Antibodies 101 animation, What... expressing this protein. Read on to learn more about antibodies and how to use them in the lab! Parts...lots is a big concern. You can find more details about polyclonal antibodies on our Antibodies 101: Polyclonal...not necessarily what you are getting. Learn more about monoclonal antibodies here. Recombinant antibodies... as well as compare between samples. Learn more about flow cytometry. Cell and tissue labeling Antibodies...

...fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at...this protein has a few more secrets - during a collaboration, members of Maureen Hanson’s and Rima Menassa's...

... 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international; varies widely by country and...educational resources.  We hope you’re as excited about this expansion as we are! Happy packaging! ...

...fluorescent proteins. Krumholz et al (Sci Rep. 2014 Feb 3; 4:3939. doi: 10.1038/srep03939.)  ... absorbance of water, which absorbs wavelengths above 900 nm or so. In other words, wavelengths between...

...gene expression and fluorescence imaging.” Cell 159(3) (2014):635-46. PubMed PMID: 25307933. PubMed Central...Avoid Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org...

... research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and ...PMID: 26780180. PubMed Central PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries Browse All...

...similarly but not identically to BE3, modifying a 3-5 base window 18 bases upstream of the PAM.  David...than BE2 Nishida et al., 2016 Target-AID   Edits 3-5 base window surrounding -18 position upstream of... efficiency and product purity. Science Advances, 3(8), eaao4774. https://doi.org/10.1126/sciadv.aao4774...Base Transversion Editors Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines... Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine base editor with improved...cell therapy applications. Dual base editors What about combining the function of both editors into one?...

...while AAV-PHP.S is typically used at a dose between 3 x 1011 and 1 x 1012 vg*. Most systemic capsids are...transduction in New World monkeys   Figure 3: Evolutionary tree depicting the phylogenetic relationship...414 (2019). https://doi.org/10.1038/s41596-018-0097-3 More resources on the Addgene blog Viral Vectors 101...Sensory Ganglion Neurons Using AAV-PHP.S. eNeuro,9(3), ENEURO.0373-21.2022. https://doi.org/10.1523/ENEURO...Are Limited to C57BL/6J Mice. Molecular Therapy, 26(3), 664–668. https://doi.org/10.1016/j.ymthe.2018.01.018... for their use. Then, we’ll provide information about commonly used and emerging capsids to help you decide...our Caltech Systemic Capsids page to learn more about that collection. Table 1: Systemic capsid plasmids...

...practicing scientists. 3)   Consider starting up your own twitter feed or talking about and sharing some science...take on collaborative roles in academia, and that means it’s important to know how to talk about science...important for scientists to get out there and talk about their work. To tell others why that work is cool... than ever before for scientists to communicate about their work and their interest in science more generally...already make it a habit to talk – really talk – about the research you are doing in the lab with your ... the details of your science but also something about how science really works. 2)   Read science writing... told. Twitter can be a good way to follow news about particular subjects or find out what others are ...

...under the United States Internal Revenue Code 501(c)(3). That means we were formed to benefit the public,...Additional Resources on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene...the American Cancer Society, or maybe you think about a local food pantry or community arts organization...community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is strength ...accelerate research with easier sharing and collaboration. Joanne and all the Addgenies: Adam, Amanda ...Connie Cepko Resources on Addgene.org Learn More About Our Mission Meet the Addgene Leadership Team Get...

...sets Taking the time to identify and research 2 – 3 potential professions will highlight which skills ...This great resource allows the undecided to learn about a variety of science professions based on their ...training focuses on using multidisciplinary, collaborative, and translational approaches for scientific...skills. Although the word “extracurricular” may be taboo in the lab, multiple research studies highlight ...social media (Twitter, Facebook, etc.) to learn about available jobs, fields, and trends “Never Leave...from your lab bench.  Want further information about shaping your career to your values, interests, and...Let us know what topic you'd like to learn more about next. Leave your suggestions in the comments section...

...signaling pathway is unknown.      Figure 3: Chimeric OptoXR incorporating rhodopsin undergoes...Ultrafast optogenetic control. Nature Neuroscience, 13(3), 387–392. https://doi.org/10.1038/nn.2495 Marshel...which tools to use, and what do you need to know about them to be successful in your experiments? In this...

...forces or (2) the result of competing desires or (3) the presence of obstacles that need to be overcome... your science with others.    My favorite quote about science ever comes from an interview in which someone...storytelling business like to say, science is all about navigating conflict. In literary terms, conflict...find that once I sit down and talk to a scientist about their research, these conflicts come out quite naturally... a simple question, like “Have you ever thought about how X works?”, or even by introducing a person that...devices, we sometimes forget the most important thing about science: It’s cool. Like bowties! Using science ...