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... also find information on using Addgene’s website, the latest Addgene news, and awards that we offer there...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...articles you want to find without even leaving the Addgene blog! Just click on the search bar at the top of...Be the first to know about new articles on the Addgene blog by subscribing to instant alerts below.   ...author pages, where you can read more about the Addgenie behind the blog post and find more of their work...interview. Get in touch by emailing us at blog@addgene.org or tell us in the comments below. We’ll track...

... be in touch with you soon with some brand-new Addgene swag!   Last month, we introduced you to Blugene...Blugene and Aavery's newest friend, our Addgene antibody mascot, here to help us with our upcoming affinity...this form or head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022...that has led them to their biggest discovery yet: Addgene will soon be distributing affinity reagents. Name... four will receive some rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig....Submissions are open until Feb. 3, 2022.  The Addgenies will choose up to four finalists, and the final...

...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning...the UK, South Korea, and beyond!   Check Out How Addgene Thinks About Developing Lab Management Software...plasmids and you decide to share them using Addgene’s repository, you can also share your associated protocols...gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular... to learn more about sharing your plasmids via Addgene? Check out all the benefits and how you can get...

...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...not an economist! Additional Resources on the Addgene Blog 5 factors to help you choose the right cloning...

...Resources at Addgene Find the ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out... al., 2016).  Bhang deposited the library with Addgene because she wanted to make it as easy as possible...and she is excited to deposit the library with Addgene because it is a “really great way of broadly sharing...sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative new ways you put ...and for providing this powerful resource to the Addgene community! Carrie Bhang is a research investigator...PMCID: PMC4900892. Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening...

...remotely instead of from the lab. Nonetheless, Addgene’s annual Deck the Lab competition drew upon the ...Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter.com/drNBUdlQco — The Tarling-Vallim...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr. Anne A Madden ...thank you to everyone who entered. Keep an eye on Addgene’s social media throughout the year for more fun ...decorations in the lab, and one for home. The Addgenies voted, and selected three winners in each category...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...adapted for detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the...editing is now possible. Learn more from this Addgene blog post Read the paper in Nature Methods Find...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...this year!  Find Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene cloned EGFP into...Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081), then generated...particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected...Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat...increased sensitivity and tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant...2025.03.20.643984.  Build your way: Explore Addgene's new empty backbones By Meghan Rego You asked, ...EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these new backbones special:...

...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub ... to use in future experiments. If you’ve used Addgene’s AAV tools, you can contribute data to the AAV ...

...As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation... for NGS-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids...are using this technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell...does not vary by DNA input amount. plexWell™ + Addgene = high quality NGS-assembled plasmids Because of...original sample. At seqWell, we have worked with Addgene to develop a highly optimized bioinformatics pipeline...circularized plasmids from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped...NGS-based assemblies for over 3000 plasmids in the Addgene collection.   Check out the SeqWell website to...

...Libraries Resources on Addgene.org Addgene's Guide to Plasmid Pooled Libraries  Addgene's Pooled Library Collection...contact us at deposit@addgene.org. Happy amplifying! More resources on the Addgene blog Genome-Wide Screening...If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an ...recombination and loss of plasmid diversity, so Addgene strives to distribute aliquots of the original ...requesting scientist receives a pooled library from Addgene, they’ll need to amplify the sample for further...generally cheaper and easier. One library that Addgene distributes with a chemical transformation amplification...Table 1: Examples of Different Pooled Libraries at Addgene.  Library Type Name Number of Plasmids ...

...You will be trying to find pictures of Addgene pets with Addgene-branded clothing on the blog. Below each...just fun and games this April Fool’s Day on the Addgene blog.  We’ve been mostly working remotely for the.... Now, we’ve collected photos of our pets with Addgene gear, and have unleashed them all over our recently...

...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog  Read more about Cpf1... will the number of reagents available through Addgene! If you're trying to decide which CRISPR tools .../nature24049 Find plasmids from this paper at Addgene. Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim...science.aaq0180 Find plasmids from this paper at Addgene. Kim D, Kim J, Hur JK, Been KW, Yoon S, Kim J-...nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y,...ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, ...nature14592 Find plasmids from this publication at Addgene. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, ...

...resources for those studying rare disease. This year, Addgene will be providing plasmid grants worth $2,000 each...BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate rare disease research...his work in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize smn1 interactions with...plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown, or overexpress these candidates...underrepresented individuals deserve a voice. Check back with Addgene often to learn of future rare disease research ...19 (2015): S64-S65. Additional Resources on the Addgene Blog Previous BeHeard Awardees Other Efforts to...Developing Countries with Seeding Labs Resources on Addgene.org Find Plasmids for Disease Research ...

...quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...organization. One thing that will remain the same is Addgene’s commitment to supporting the research community...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger...

...announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries...reagents available to the research community through Addgene and look forward to another great year of sharing...PubMed Central PMCID: PMC4486245. Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral Guide Pages Find ...

...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube! In this series...

...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology.... coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters just ...microbial technology, sign up to write about it on the Addgene blog. ...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...organisms we should include by emailing us at help@addgene.org. If you are looking for plasmids used in E. ...

...blog@addgene.org.  Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...decided to share some of our tips in our new series: Addgene Lab Tips! In each short episode of this series...Science: We Share Science Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol...