We narrowed to 398 results for: aga

1. Overcoming the AAV Size Limitation for CRISPR Delivery

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   Blog Post

   ... Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH, Moineau S (2010) The CRISPR/Cas bacterial immune...

2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional...

3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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   ...D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent...

4. Deciphering the Mysteries of Behavior with Viral Vectors

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   Blog Post

   ...open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural...

5. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   ...squash" originated from the squash itself, but think again. Spaghetti squash is actually a component of the...

6. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   ...favor a different model. Finding evidence for or against a model is a standard scientific strategy. However...

7. Bioinformatics at Addgene

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   Blog Post

   ...QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow   Over the years we have...

8. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   Blog Post

   ...Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral supporters Denise...

9. CRISPR-based Genome Editing Systems For Editing in E. coli

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   Blog Post

   ...Increasing rounds of transfer on transposition-inducing agar lead to higher cargo copy numbers. (D) The effect...

10. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...synthesis, and the neomycin resistance gene protects against any of them. But different aminoglycosides work...

11. CRISPR 101: Any Base Transversion Editors

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    ...other transversion editors be close at hand? Once again, this post has a lot of acronyms, so we've included...

12. Tips for Writing a Good Cover Letter

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    Blog Post

    ...manager may have. The closing paragraph should again show your enthusiasm for getting the position and...

13. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...RepB) necessary for replication in various hosts (Pagatto and Dillon 2001).  The plasmid has its own replication...

14. Antibodies 101: Reading a Flow Plot

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    Blog Post

    ...different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs are CD11c...

15. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post

    ...as of October 1st the salary minimum was raised again to $51,600, itself a raise of 3.2%. Other institutions...

16. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...

17. Tips for Getting a Faculty Position

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    Blog Post

    ...create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"...

18. Hot Biosensors 2022: Year-End Roundup

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    Blog Post

    ...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded...

19. Five Popular Model Organisms, Part 2

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    ... to be replicated. Because E. coli are easily propagated and have a generation time of around 20 minutes...

20. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post

    ...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent...