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We narrowed to 643 results for: ato

Showing: 321 - 340 of 643 results

  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...own replication machinery such as Rep or other initiator proteins, they do not require host proteins for...

  3. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar and Marty, Jean...

  4. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

  5. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

  6. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus 5-derived...usually provided by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan....

  7. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration can often be seen by...

  8. Hot Plasmids - November 2023

    Type
    Blog Post
    ...example, neurons treated with a phorbol ester activator gave fluorescent signal in the ticker tape from...transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD...

  10. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

  11. March for Science

    Type
    Blog Post
    ...know is your area code. Senators: To find contact information for your senators all you need to do is select...

  13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...line and ~8 and 9% efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers ...

  14. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...Often, however, researchers lack knowledge of the regulatory kinase, access to active kinase for the reaction...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

  16. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...academic publishing goals and make you a better communicator overall. Don’t Write in a Vacuum Your research...through many iterations read by your PI, your collaborators, others you know in the field, and even people...

  17. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would...

  18. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

  19. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector...

  20. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...
Showing: 321 - 340 of 643 results