We narrowed to 376 results for: bre

1. Plasmids 101: Colony PCR

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   Blog Post

   ...is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR...

2. Tips for Screening with Yeast Two Hybrid Systems

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   ...Science. 1986; 231:699-704. PubMed PMID: 3080805. 3. Brent, R., and Ptashne, M. A eukaryotic transcriptional...

3. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   Blog Post

   ...facility). The third tube is used to fulfill orders. Breaking the (bar)code At each step in the receiving, transforming...

4. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...small victory in a long road to your scientific breakthrough. More Reading: How to manage scientific data...

5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...studies, act as a reporter for DNA double strand break repair, and to label pathogenic intracellular microbes...

6. Your Lentiviral Plasmid FAQs Answered

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   Blog Post

   ... insert encoded by the vector." The table below breaks down the biosafety concerns associated with various...

7. Negotiating Work and Life: How to Find the Joy

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   ...exception is that men cannot actually give birth and breastfeed. Keeping this always at the forefront will go...

8. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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   ...first conference room, that is actually used as a break room for Addgenies, was named after the protein ...

9. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   Blog Post

   ...it in the cleaved complex. This results in DNA breakage and cell death. Conversely, CcdA binds to and ...

10. Finding nucleic acids with SHERLOCK and DETECTR

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    ... generates 5’ and 3’ staggered ends after dsDNA breaks. The Doudna lab discovered Cas12a’s ability to ...

11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...matching Mentors with Singletons for short coffee breaks. While this was not the more intense mentorship...

12. An Inside Look into Addgene's Dev Team

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    ...collaborative approach to development. Scrum teams plan and break up work into small, well defined chunks, called ...

13. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...MECP2 gene, and highlights some of the latest breakthrough therapeutic approaches. What is Rett Syndrome...

14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ... Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation of “...

15. CRISPR 101: Any Base Transversion Editors

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    ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...

16. Hot Plasmids: Fall 2024

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    .... abscessus. A) Sth1Cas9 mediates double strand break at the target site, which results in either bacterial...

17. Deciphering the Mysteries of Behavior with Viral Vectors

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    ....12 (2007): 1079. PubMed PMID: 17700574. Hare, Brendan D., et al. "Optogenetic stimulation of medial prefrontal...

18. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ... genetic resources and highlight two recent breakthroughs in developmental biology that arose from zebrafish-based...

19. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ... (Virginia Weis) Sea anemone Aiptasia - algae Breviolum minutum symbionts are important models for solutions...

20. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...engineering single genome edits but the double strand breaks it introduces makes it difficult to perform two...