We narrowed to 636 results for: erf
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TypeBlog Post...discusses some of the key things to consider when performing colony PCR. Designing colony PCR primers ...Choosing a Molecular Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive...
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Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...measured to quantify (indirectly) transcription performed by the viral polymerase. Alternatively, the mRNA...independently. In addition, experiments can be performed in the absence of some viral proteins (compared... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...information. Although two-hybrid systems are a powerful tool for screening molecular interactions, accuracy... and reproducibility are common problems when performing these experiments. False positives can occur ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...wavelength (yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) K26R, Q80R... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post... review click here). Alternative envelopes are powerful tools as they allow researchers to target transduction...with respect to the specific experiments being performed and you should understand the guidelines for using... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post... the ICE tool by analyzing thousands of edits performed over multiple experiments and comparing the robustness...search for a particular sequence or name. You can perform more in-depth analyses on each sample by clicking... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post... subsequently reproduce the research. A third, powerful example is the integration with protocols.io, ...DSpace repository. These integrations utilize an interface that enables users to conveniently select and ... -
Hot Plasmids - November 2023
TypeBlog Post.... These variants use different RT enzymes and performed large edits with similar or better efficiency ...we began distribution and has been verified to perform well in standard IHC and western blot protocols... -
Hot Plasmids: Fall 2024
TypeBlog Post...C-terminus (or even in the middle of proteins) and performs well in many different antibody-based applications...high rate of spontaneous antibiotic resistance interfering with selection. Using an inducible Cas9 expression... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...Katrin Michel. Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but it...Buchholz, Angrand, and Stewart 1998). FLPe’s performance was further improved, by adding a nuclear localization... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...chemotherapy (HIPEC). With this technique, surgery is performed to remove the mesothelioma tumors from the peritoneum...to which it may have spread. Then, the surgeon performs a heated chemotherapy wash throughout the abdominal... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...which ones aren't? This video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria...testing for antibiotic resistance isn’t commonly performed because resistance in these infections is so rare... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...pressure to maintain consistently high levels of performance, and occasionally being faced with “shifting ...crucial to how our clients’ products will end up performing in the market. Hence, as a PhD, an MD, or someone... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...on the fly”. This becomes intuitive with touch interfaces where the viewer is more likely to want to manipulate... to Research Data? - by Francine Berman and Vint Cerf (Science, August 2013) PLOS’ Bold Data Policy - ... -
Five Popular Model Organisms
TypeBlog Post... can also be easily knocked-down through RNA interference (RNAi) by feeding the worm bacteria expressing... Andrew, et al. "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...molecular processes that make aptamer biology a powerful tool. In this post, we’ll take a deeper dive into... Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...vector had an improvement in muscle physiological performance. 2. Trans-splicing Overview: For trans-plicing...et al., 2005). Trans-splicing does not always outperform single oversized AAVs (Hirsch et al, 2013). ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...time-consuming purification processes, and are powerful, due to their ability to integrate into the host...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...semi-quantitative western blots. No pipetter is perfect, and proteins may not transfer precisely evenly... and gel quantification. Figure 3: In a perfect world, protein concentration in your sample would...