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  1. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to... modified to tag hundreds of genes, generating powerful genetic screening libraries. Additional protein...

  2. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...every work setting and no manager will ever be perfect, but the chances of getting a good manager do tend...at once. Many companies will hire a postdoc to perform a specific project, while others will hire a group...

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed...plasmids as part of quality control, but does not perform functional tests. The newest genome engineer sharing...

  4. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...from organism to organism. MMEJ doesn’t yield a “perfect repair” like HDR, but it’s much more predictable...ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter, and the...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...discuss how CRISPR libraries are being used to perform genome-wide screens and highlight some of the reagents...What are the steps involved in a CRISPR screen? Performing a forward genetic screen using CRISPR libraries...

  6. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...belongs to the penicillin family of antibiotics. It interferes with cell wall synthesis as its mode of action...Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    ... when they begin flowering. It is important to perform the transformation not long after flowering begins...2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in ...

  8. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...discusses some of the key things to consider when performing colony PCR.   Designing colony PCR primers ...Choosing a Molecular Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...information. Although two-hybrid systems are a powerful tool for screening molecular interactions, accuracy... and reproducibility are common problems when performing these experiments. False positives can occur ...

  12. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ... review click here). Alternative envelopes are powerful tools as they allow researchers to target transduction...with respect to the specific experiments being performed and you should understand the guidelines for using...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...C-terminus (or even in the middle of proteins) and performs well in many different antibody-based applications...high rate of spontaneous antibiotic resistance interfering with selection. Using an inducible Cas9 expression...

  14. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...chemotherapy (HIPEC). With this technique, surgery is performed to remove the mesothelioma tumors from the peritoneum...to which it may have spread. Then, the surgeon performs a heated chemotherapy wash throughout the abdominal...

  15. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...

  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ... the ICE tool by analyzing thousands of edits performed over multiple experiments and comparing the robustness...search for a particular sequence or name. You can perform more in-depth analyses on each sample by clicking...

  17. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... subsequently reproduce the research. A third, powerful example is the integration with protocols.io, ...DSpace repository. These integrations utilize an interface that enables users to conveniently select and ...

  19. Hot Plasmids - November 2023

    Type
    Blog Post
    .... These variants use different RT enzymes and performed large edits with similar or better efficiency ...we began distribution and has been verified to perform well in standard IHC and western blot protocols...

  20. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...which ones aren't?     This video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria...testing for antibiotic resistance isn’t commonly performed because resistance in these infections is so rare...
Showing: 321 - 340 of 631 results