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...the relevant tag and then introducing a specific ligand to mark the tag and whatever it is fused to for...expressed, protein levels will quickly recover once the ligand is removed. Degron tag systems include small molecule...

...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...

...viral services. After a month or so of meetings, I began to notice something: even though our scientists ...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions...

...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

...as inhibitory neurons, rods, cones, or retinal ganglion. Rather than using naturally occurring promoters...Cre-dependent-GFP expresses in horizontal cells and ganglion cells, only the horizontal cells transduced by...

...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...

...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...

...mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating fluorescent protein .../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources...

...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1...

...work or volunteer for a science communication organisation like Building with Biology. Local environmental...You can draw live sketches at conferences like @ATJCagan or create agar art with colorful microbes? Try...

...you can accomplish this by employing a neat and organized format.  Columns. You can choose from either ...Mike Morrison, a psychology doctoral student at Michigan State University. The idea is that the main research...

...Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...

...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ...

...lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi Gan 186221 pBMN BFP-Parkin PRKN BFP Parkinson's Michael...Frontotemporal dementia in GRN mutation carriers Michele Pagano 210366 TMEM106B-EGFP TMEM106B GFP CMV, T7 Frontotemporal...Frontotemporal dementia in GRN mutation carriers Michele Pagano 210899 pFBOH-MHL_LRRK2:2141-2527 LRRK2 His polH...pFastBac-GST-TBK1 TBK1 His, TEV, GST polH ALS Jonathan Kagan 221522 SYNJ1_BirA_Cterm SYNJ1 BirA, Flag CMV Parkinsonism...

...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...

... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...techniques: the powerful tools for spatial genome organization deciphering. Molecular Cytogenetics, 11. https...

...sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may be that chromoproteins...symbiotic algae, during heat stress. For other organisms, like the sea anemone, scientists think that chromoproteins...

...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which will...target protein and an E3 ligase through interacting ligands. After an E3 ligase facilitates ubiquitination ...

...this bottleneck, we began building CrispyCrunch in July 2018 and in November, we began using it internally...

... produce all of the differentiated cells in an organism, including the embryonic and extraembryonic cell...quantities in specific “stem cell niches” found in many organs in the adult human body. These generate specialized...