We narrowed to 614 results for: gan

1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ...

2. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   ...the relevant tag and then introducing a specific ligand to mark the tag and whatever it is fused to for...expressed, protein levels will quickly recover once the ligand is removed. Degron tag systems include small molecule...

3. All in a Twist: dsRNA

   Type

   Blog Post

   ...previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA ...cellular dysregulation) is the mitochondria. These organelles have their own circular genomes which can produce...

4. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   ...understanding of the physiology and pathology of the basal ganglia in movement disorders.   References and Resources...protein-coupled receptors potently activated by an inert ligand. Proceedings of the National Academy of Sciences...

5. RNA Extraction Without A Kit

   Type

   Blog Post

   ...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...

6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer...

7. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   ...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

8. TALEN Plasmids and Kits

   Type

   Collection

   ...Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector...

9. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   ...you can accomplish this by employing a neat and organized format.  Columns. You can choose from either ...Mike Morrison, a psychology doctoral student at Michigan State University. The idea is that the main research...

10. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1...

11. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    ...work or volunteer for a science communication organisation like Building with Biology. Local environmental...You can draw live sketches at conferences like @ATJCagan or create agar art with colorful microbes? Try...

12. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...

13. Lab Automation at Addgene

    Type

    Blog Post

    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...

14. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    ...as inhibitory neurons, rods, cones, or retinal ganglion. Rather than using naturally occurring promoters...Cre-dependent-GFP expresses in horizontal cells and ganglion cells, only the horizontal cells transduced by...

15. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    ...mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating fluorescent protein .../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources...

16. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    ...Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...

17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    ...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ...

18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    ... produce all of the differentiated cells in an organism, including the embryonic and extraembryonic cell...quantities in specific “stem cell niches” found in many organs in the adult human body. These generate specialized...

19. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    ... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...techniques: the powerful tools for spatial genome organization deciphering. Molecular Cytogenetics, 11. https...

20. Transferable Skills: Negotiation

    Type

    Blog Post

    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...