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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11 results for: gan

Showing: 1 - 11 of 11 results

  1. Chemogenetics Guide

    Type
    Guide
    ...small molecule ligand. The ideal chemogenetic tools are unresponsive to native ligands and are engineered...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...unresponsive to endogenous ligands, could be activated by synthetic ligands. Specifically, Receptors Activated...insensitivity to endogenous ligands, have low constitutive activity, and their activating ligands have few, if any... Figure 2: DREADDs, their ligands, and signaling properties. DREADD Ligands Muscarinic-receptor based ...neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially important... Activated Solely by Synthetic Ligands (RASSLs) based on κ-opiod receptors (Coward et al. 1998). These...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...optimal serotype(s) for transduction of a given organ. Tissue Optimal Serotype Central nervous system ...administration, distributing widely throughout the organism to target large or diffused biological structures... of systemic rAAV vectors that can target whole organs or specific cell types of the CNS (e.g. AAV-PHP.eB... transport Movement of molecules, vesicles or organelles from the main cell body, or soma, towards the...pathogen, like viruses or bacteria, within a host organism, leading to disease. Insertional mutagenesis Random...

  3. Molecular Biology Reference

    Type
    Guide
    ...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...when trying to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would ...

  4. Plan Your Experiment

    Type
    Guide
    ...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...carry out your experiment in your particular model organism. There may not be a perfect plasmid for your specific...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...sequence and design your gRNA. Know your cell line/organism and genomic sequence When possible, you should...may vary depending on the specific cell line or organism, which may affect the observed efficiency of CRISPR...

  5. CRISPR Guide

    Type
    Guide
    ...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG...Hultquist, J. F., Waters, C. S., McGregor, M. J., Krogan, N. J., & Bondy-Denomy, J. (2017). Inhibition of..., E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan, D., Braff, J. L., Davidsohn...23907171 Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,...

  6. Optogenetics Guide

    Type
    Guide
    ...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...LAD Light-activated dimerization, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage (LOV) domain...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Hussain, R., Banani, M. A., Day, W., Stevenson, E., Madigan, M., Chen, J., Khinder, R., . . . Pule, M. (2022...pathogen, like viruses or bacteria, within a host organism, leading to disease. Provirus The genetic material...

  8. Lentiviral Vector Guide

    Type
    Guide
    ... Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996...pathogen, like viruses or bacteria, within a host organism, leading to disease. Lentivirus A retrovirus from...

  9. Antibody Guide

    Type
    Guide
    ...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

  10. Promoters

    Type
    Guide
    ...promoter region controls when and where in the organism your gene of interest is expressed. Summary Promoters...

  11. Adenovirus Guide

    Type
    Guide
    ...pathogen, like viruses or bacteria, within a host organism, leading to disease. Innate immunity Non-specific...
Showing: 1 - 11 of 11 results