We narrowed to 933 results for: mpl

...appropriate time—for example, the cell must have enough nutrients available to complete the entire round ...and regulation in bacteria Before looking at an example plasmid, let's review how E. coli normally replicate...chromosomal replication. Adapted from BioRender template. Since DnaA binding is necessary to initiate... stringently regulate plasmid replication. For example, the plasmid ColIb-P9 encodes the protein RepZ ...Inc, also encoded by the ColIb-P9 plasmid, is complementary to part of the sequence forming this pseudoknot...replication. At high copy numbers, RepA proteins form complexes between iterons on different plasmids in a process...

...whole-genome/transcriptome” studies, in which, for example, the activity of all genes in response to an experimental...the globe (Nüsslein-Volhard et al., 2012). One example (which I will come back to) is the one-eyed pinhead...mutant, which regulates a developmental pathway implicated in congenital heart defects and cancer. These...morpholinos. When injected into zygotes, morpholinos complementary to a target mRNA inhibit its translation either...genome is useful for a variety of applications. For example, specific tissues such as blood cells or neurons...regulatory systems have also been successfully implemented in zebrafish. Developmental biology insights ...well-suited for neuroscience investigations. For example, neuronal activity can be tracked in whole brains...

...fields in order to provide useful solutions to complex challenges. As a cross-disciplinary researcher,...Many of the challenges in science today are too complex for any single discipline to solve them on their... in terms of communicating ideas. In 2012, for example, 3 billion hours of video were watched each month... disciplines leading the way, the future of complementing published research with video overviews was ...when it is of quality). On the site you can, for example, learn about how researchers are using “big data...contribution. It offers a 5-step process and several examples for creating effective research videos.  Karen...derived from research. The platform uses video to complement traditional print journals and expand our ability...

...certain loci to create changes in phenotypes. For example, CRISPR activation has been used to differentiate...gRNAs targeting every gene in the human genome for example, can be made easily and cheaply using oligo library...change the sequence targeted by the activator by simply providing a new gRNA rather than engineering an...allows for exploratory-based research as well. For example, CRISPRa can be used to find potential cancer drug...transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136...Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

...coming weeks, but, in this post, we will outline a simple method for selecting the guide RNA, validating ...CRISPR Design tool supplied by the Zhang lab. This simple website invites you to input the target region ...happy with your selection, ordering the sgRNA is simple and easy. Several companies supply gRNA sequences...you gRNA in vitro first. In vitro screening is a simple and fast method for validating your selected gRNA...base pair sequence surrounding your gRNA. Once amplified, you can insert this region into a pCAG-EGXXFP...). Testing the efficacy of your gRNAs is then a simple matter of transfecting HEK293T cells with your ...perfect primer sets to use to genotype your pups. A simple PCR with these primers will let you know if there...

... used as the first letter of a plasmid name and simply denotes that the object is a ‘plasmid’. “p” is ... empty backbone name in your plasmid name. This simple piece of information can often convey many important...insert abbreviation to specify what species it is. Example: ‘h’ is for Human (homo sapiens), ‘m’ is for mouse...plasmid and their relative position to the insert. Example, if you have a Flag tag on the N-terminal of your...directly before or directly after the insert name.   Example: If the Glutamine at position 295 was mutated to...pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt)-EGFP These simple rules allow any scientist to know what is in a ...

...undergraduate experiences, they were provided with ample resources and a positive perspective on the transition...career path of their choice. This failing becomes amplified by systemic biases which, in the absence of support...from all areas of the scientific training and employment infrastructure. A Stakeholder Workshop at Janelia...career training approaches in a way that can be implemented widely in the community.  Talk alone is not going...resulting in concrete actions and outcomes. For example, pd|hub assembled a coalition of higher education...Sciences (NIGMS) will support the development and implementation of collections of evidence-based practices....

...confrontational situations with others because you simply cannot see things from their point of view and ...herself to the nearby village (and to the reader) by simply listening. Chances are, you know someone like this...teams resolved similar issues in the past, or how completely different teams are tackling comparable conflicts...it will be important to retain the stories and examples that have guided your resolution processes in ...collaboration may not be the easiest resolution to accomplish, but there is a potential for long-term gains...into your conflict management skills. It’s also completely OK to recognize that conflict resolution may ...or are looking for a promotion, have some solid examples of times when you have used your empathy, listening...

...this tradition. We now have over 30 full-time employees, so we created one long lunch table and we eat...about your colleagues on a personal level, and employees often cite lunch as one of their favorite parts...provide free food on special occasions. However, employees regularly bring in baked goods and we have a few...To-Do list long enough that there is no hope of accomplishing it all. How do we encourage people to take a... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...projects. Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional...

...sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT, with AAV vectors are common...vectors, and post-translational regulatory elements. Examples of these approaches are given in Tables 3-7. 3...specificity of cell targeting even further. For example, injecting AAVs at one location can result in expression...that received information from a region. In the example shown in Figure 1, injected retrograde AAVs at ...neuroscience is becoming more and more versatile. To complement the wealth of genetically encoded tools available...only reagents, but also method information. To complement these efforts, we’re beginning to share data ...

...also enables the excision of the transposon in a completely seamless manner, leaving no sequences or mutations...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...challenges still remain, such as making gene edits completely seamless (i.e., so no other genetic alterations...leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing a selection...included in a standard homology directed repair template to facilitate the selection of cells containing...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...

...hand. Records of plasmid construction were often incomplete or nonexistent. In the 21st century, many molecular...molecular biologists didn’t know the complete sequences or properties of the DNA molecules they were using...what the user wants to do and make the path to accomplishing their goals as intuitive and painless as possible...refinements in response to customer feedback. An example of this approach is SnapGene’s algorithm for detecting...could annotate a feature inaccurately. The best example is fluorescent proteins, which often come in closely... Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene Browse All...

...not powerful enough for large brain samples. For large brain samples, we recommend pretreatment with ScaleCUBIC1...the stochastic expression of XFPs is possible by simply introducing a mixture of XFP genes. Stochastic ...time points, sacrifice the animals and fix the samples. When you use AAVs, it typically takes a few weeks...excessive amount of XFPs expressed. Then, clear the samples and visualize. For more details on this procedure..."Optical clearing and index matching of tissue samples for High-resolution fluorescence imaging using ...

...which has humanistic and philosophical implications. As an example, a group at Caltech used an optical fiber...and what it can enable? Boyden: There are many examples where you want to activate two sets of neurons...quarter of a million people have some kind of neural implant already. If we can use light to more specifically...Those are two of the biggest ones. And as a third example, it would be helpful to have molecules with better...three or four hour conversation. The brain is very complex and we've hosted hundreds of visitors in our lab...

... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter... data gathering, regulatory efforts, policy implementation, program management, communications, diplomacy...setting: drafting legislation, making rules, and implementing the policy within the jurisdiction of the governing...working in science policy doesn’t have to be a complete career pivot. There are many ways to pursue your...proposals. Check out the Day One Project for some example proposals and consider applying for their accelerator...expertise and then once selected, you can pivot to a completely new field without issue. This may be more challenging...

... a photosensitizer composed of the protein complex complex and the MG-2I-dL5** fluorogen that can be used...thus forming the full FAP-TAP complex (MG-2I-dL5**).   A FAP-TAP complex will light up if excited with...-TAP MG-2I-dL5** MG-2I-dL5** is a new FAP-TAP complex where dL5** is the FAP. It’s job is to bind MG-...fluorogenic dye.  MG-2I is the TAP portion of the complex and is derived from the malachite green (MG) dye...

...non-trivial, however, as FPs are tremendously complicated entities with a large range of characteristics...about FPs than is currently represented at fpvis (complete spectral information, accession numbers, sequences...the goal of encouraging user contributions (using simple web forms) while also maintaining a relatively ...register with a valid email address).  One may also simply attach important references that serve to further...more powerful ways, and the advanced search page simplifies the process of building advanced multi-parametric... few years with an emission peak around 600nm?  Simply input these parameters and you’ll find matching...

...the requesters what they needed. In the example above, for example, we fostered a formal scientific collaboration...uploaded to the preprint repository BioRxiv. This example clearly illustrates the power of collaboration,...certain projects. The idea behind the platform was simple: any person working on COVID-19 could file a request...groups. In other cases, the collaborations were simpler and the volunteers helped with things like translations...need help with your scientific research. It’s completely free, of course. You can find more information...

...cell and any individual AAV particle is rare. But simply getting into the cell may not always be enough ...and therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s ...titers for successful transduction. In another example, neuronal gene expression by an anterograde AAV...visualize expression of fluorescent proteins, for example, as many viral particles must infect a cell to ... can optimize AAV production. We’ve listed some simple steps you can take below but you should read our...possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles to ...

...started the process with, we got great amplification of our sample libraries. NEB also gave us a few goodies... aim to do large scale DNA preps with multiple samples without cross contamination. We currently use the...create a DNA library, one must first fragment a DNA sample into many small components. Without proper processing...repair Adapter Ligation Clean up/Size selection Amplification Clean up  I found this kit easy to use because... I am bombarded with a lot of information. For example, before going to my next workshop, I’ll ask myself...