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We narrowed to 14 results for: mpl

Showing: 1 - 14 of 14 results

  1. Antibody Guide

    Type
    Guide
    ...fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain fragment...application Collect and lyse samples to make proteins available. Tissue samples may need additional processing...proteins from a complex mix. Overview of application Process the tissue or cell culture samples to extract ...capture protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced...sequenced and amplified for analysis. Overview of application Process tissue or cell culture samples to extract...and lyse. Cross-link the samples with formaldehyde or UV light. Shear the samples using sonication to break...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...

  2. CRISPR Guide

    Type
    Guide
    ...enzyme by simply changing the target sequence present in the gRNA. CRISPR was originally employed to knock...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...experiments and must be amplified before use (Figure 8C), following the amplification protocol specified by...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most...

  3. Optogenetics Guide

    Type
    Guide
    ...Human codon optimization Example: Halo Increased photocurrent amplitude Examples: eNpHR, eNpHR2.0, eNpHR3.0...Increased light sensitivity Example: ArchT Increased photocurrent amplitude Examples: eArch3.0, eArchT3.0 Browse...enhancements include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ChIEF Increased ...) kinetics: Examples: ChETA, C1V1(t/t), ChrimsonR Red-shifted peak action spectra Examples: VChR1, C1V1...enhancements include: Increased photocurrent amplitude Examples: iChloC, SwiChRca, Phobos, Aurora Browse ...enhancements such as: Improved photocurrent amplitude Example: eMac3.0 Browse Leptosphaeria plasmids . Glossary... cells in order to understand their functions. Example schematic of an optogenetics procedure. A channelrhodopsin...

  4. Molecular Biology Reference

    Type
    Guide
    ... amplified again producing ~1,000 copies of each template. Each template is then replicated using the ...of DNA, or templates, are spatially separated from each other. These attached DNA templates are then amplified... vary broadly in their functionality. In their simplest form, plasmids require a bacterial origin of replication... for the ability of the plasmid to be copied (amplified) by bacteria, which is an important characteristic...sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers can... using bacteria, which can uptake plasmids and amplify them during cell division. Because bacteria are... DNA fragments. Cloning vectors tend to be very simple, often containing only a bacterial resistance gene...

  5. Guide to Using Pooled Libraries

    Type
    Guide
    ...see if it should be amplified before you conduct your screen. If you need to amplify the library, please...recommended to verify that the library is complete - an incomplete library may lead to false positives or...types of screen employ a selection method relevant to the phenotype being studied. Examples of published ... Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read ...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens...: Pooled Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to...genes that contribute to a phenotype. At their simplest level, pooled libraries are single preparations...

  6. Cloning

    Type
    Guide
    ...ends in sequences that form complex secondary structues. You can view an example of a LIC protocol on our...discovery. With molecular cloning scientists can amplify and manipulate genes of interest and then insert...overhang (blunt ends). Two pieces of DNA that have complementary overhangs, or which are both blunt-ended, can...restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested and ligated...a single recombination reaction, drastically simplifying the process and reducing the amount of time required...). To do this your DNA fragment must first be amplified with specific Gateway attB1 and attB2 sites attached...selection cassettes). Although gateway cloning is a simple and efficient cloning method, gateway vectors and...

  7. Plan Your Experiment

    Type
    Guide
    ... donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing...edits using CRISPR/Cas9 in mammalian cells as an example, but many of these principles also apply to using... process of interest. Do you want to: Generate complete and permanent loss of gene expression or function...Remember that HDR also requires donor DNA to serve as template for the repair. Once you settle on your gRNA, ...Editing : pegRNAs must serve as both a guide and a template. In general, edits must be downstream (3’) of ...edit site yield higher editing efficiencies. The template sequences can also have a large impact on editing...perfect world, your gRNA sequence would be an exact complement to your target sequence with no homologous sites...

  8. Promoters

    Type
    Guide
    ... transcription is simpler than eukaryotic transcription bacteria still have complex systems of gene regulation...promoters, transcription and the transcription complex. Plasmid...produced. The antisense strand is referred to as the template strand or non-coding strand as this is the strand...other hand, the process in eukaryotes is much more complex. Eukaryotes require a minimum of seven transcription...promoter region stably and transcription of the template strand can initiate. The TATA box is a DNA sequence...Transcription Eukaryotic promoters are much more complex and diverse than prokaryotic promoters. Eukaryotic...away from the TSS. Eukaryotic promoters are so complex in structure that the DNA tends to fold back on...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...as adenovirus or herpes simplex virus to replicate in the host cell and complete the lytic cycle. AAVs ... acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at ...transduction efficiency and alter tropism. For example, AAV2/5, targets neurons that are not efficiently...transduction efficiency and viral tropism. One common example is AAV-DJ, which contains a hybrid capsid derived...Several methods have been developed and successfully employed to engineer new rAAV capsid variants, such as ...retrograde (AAVrg) Directed evolution has also been employed to engineer an rAAV serotype variant able to display...blog post on AAVrg for more information. Self-complementary AAV (scAAV) One downside of the use of AAVs ...

  10. Modular Cloning Guide

    Type
    Guide
    ...restriction cut site, multiple modules with complementary overhangs can be digested and ligated in a single...vector creating a single transcriptional unit (for example, a promoter, a 5' UTR, a signal peptide, a protein-coding...Level 2 vectors permits the creation of even more complex constructs constrained only by the ability of E...overexpression, gene editing, functional genomics, and more complex metabolic engineering and synthetic biology, compatible... MoBiFC - Modular Bimolecular Fluorescence Complementation Toolkit Plant Expression Benjamin Field 50 ...plasmids for assembling bimolecular fluorescence complementation experiments to analyze protein-protein interactions...toolkit with both high cloning capacity and vector simplicity, compatible with other MoClo, GoldenBraid, and...

  11. Adenovirus Guide

    Type
    Guide
    ... acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at ...due to their well-established methods, relative simplicity of production, and higher titers. However, the...also resulted in a reduction of viral vector amplification and overall lower viral titers. Because of this...transduction efficiency, tropism and immunogenicity. For example, Ad5/35 and Ad5/26 combine the high transduction...packaging capacity. Large protein-coding genes and complex gene expression cassettes are often too big to .... K. (2011). Adenoviral vector immunity: its implications and circumvention strategies . Current Gene ...., Kinzler, K. W., & Vogelstein, B. (1998). A simplified system for generating recombinant adenoviruses...

  12. Chemogenetics Guide

    Type
    Guide
    ...any off-target effects. They are the most widely employed type of chemogenetic receptors. There are several..., and hM5Dq, however, hM3Dq is the most widely employed. Gi-DREADDs signal through the Gαi/o G-protein...allowed them to respond to their cognate PSEM. For example, PSAM Q79G,Q139G is activated by PSEM 22S , while...will determine the chemogenetic ligand used. For example, a study in rats using an hM4Di receptor might ...adrenergic receptor to engineer a receptor-effector complex for gene therapy. J Biol Chem. 276:1596-6. PMID...

  13. Lentiviral Vector Guide

    Type
    Guide
    ... acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at ...hence the use of “retro”. Lentiviruses have more complex genomes, containing packaging genes and accessory...along with virus-specific accessory genes (for example, vif , vpr , vpu , and nef for HIV-1). The LTRs...Timms et al. (2018), and Wells et al. (2020) for examples). Pseudotyping Tropism dictates which types of...

  14. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at ... — hence the use of “retro”. Retroviruses have simpler genomes, containing only the necessary packaging...transfection step of the viral packaging cell line. For example, Phoenix™ is a popular second-generation gamma-...
Showing: 1 - 14 of 14 results