We narrowed to 457 results for: nsf

1. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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   Blog Post

   ...possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory body...

2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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   ...transcription in response to river contaminants and transform these plasmids into a bacterial strain. The sensor...

3. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   ...on cellular processes. For help with library transformation and amplification, refer to the Zhang lab’s...

4. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   ... that are an order of magnitude greater than transfection approaches. Moreover, the cost of reagents is...

5. Addgene Welcomes John Doench to the Advisory Board

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   Blog Post

   ...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene...

6. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   ...an unregulated fashion and thus a marker for transfection. The other (mKate2) is regulated in the 3’ UTR...

7. GPCRs: How Do They Work and How Do We Study Them?

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   ...activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling...

8. A Deep Dive into BioBuilder

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   ...cutting-edge research projects in synthetic biology and transforms them into modules that students and teachers ...

9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent protein...

10. A Quick Guide to a Career in Software Product Management

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    Blog Post

    ...explaining more of her role, which eventually led me to transfer to her team and begin my product management (PM...

11. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ...information (more on this below). Plating the transformation on ampicillin plates containing X-gal allows...

12. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ...sequencing as a first pass QC. The lab team manually transforms, innoculates, and performs DNA minipreps for ...

13. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ... host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a plasmid...

14. Screening for Successful Genome Editing with Digital PCR

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    ...PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates...

15. Addgene’s Viral Service - Why Virus? Why Now?

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    ...scientists and the support of their technology transfer offices, Addgene would not be able to have such...

16. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post

    ...IF-ICC.) This method was chosen because cells transfected with a plasmid to express the target antigen ...

17. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    ...Scientists: What Makes a Good Leader Anyways?  Transferable Skills Guide: Managing a Team  ...

18. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post

    ...of cell types that are typically ‘difficult to transfect’, such as primary cells. They can also be directly...

19. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ...note that due to the size of the backbone, transformations require electroporation. References & Further...

20. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post

    ...cells after the plasmids have been introduced by transfection or electroporation. All you do to create a protein...