We narrowed to 400 results for: pho

1. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

2. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

3. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

4. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

5. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...form teratomas [11]. iPSCs also possess similar morphologies, growth properties, and express stem cell markers...

6. Genome-wide Screening Using CRISPR

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   Blog Post

   ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

7. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   Blog Post

   ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

9. Great Results Start with Great Standard Curves

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   Blog Post

   ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

10. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

11. Plasmids 101: Protein tags

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    Blog Post

    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

12. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

13. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post

    ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...

15. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post

    ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

16. Hot Plasmids Spring 2024

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    ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

17. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

18. Hot Plasmids - November 2023

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    Blog Post

    ... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

19. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...

20. Career Insights: Technical Support Specialist

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    Blog Post

    ...support roles are primarily office based, and email/phone are the most popular ways to communicate with customers...