We narrowed to 400 results for: pho;

1. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   ...’d like us to discuss. We are also available by phone or at [email protected] to answer your questions ...

2. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   ...using cloning rings in terms of growth rates, morphology or other physiological properties. An advantage...

3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   ...mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it takes roughly...

4. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

5. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

6. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

7. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

8. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

10. Great Results Start with Great Standard Curves

    Type

    Blog Post

    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

11. Plasmids 101: Protein tags

    Type

    Blog Post

    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

12. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    ...provided moral support was invaluable.  Mixing metaphors and scientists - Culturing a scientific network...

13. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    ... regulated by guanosine [corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death...

15. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

16. Hot Plasmids - November 2023

    Type

    Blog Post

    ... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

17. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

18. Hot Plasmids Spring 2024

    Type

    Blog Post

    ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

19. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

20. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...