We narrowed to 407 results for: pho;

1. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...form teratomas [11]. iPSCs also possess similar morphologies, growth properties, and express stem cell markers...

2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   ... of the highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient ...

3. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

4. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...’d like us to discuss. We are also available by phone or at [email protected] to answer your questions ...

5. Cloning Mammalian Cells with the Agarose Method

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   ...using cloning rings in terms of growth rates, morphology or other physiological properties. An advantage...

6. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it takes roughly...

7. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

8. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

9. Degrading DNA with Cascade-Cas3

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   ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

10. Hot Plasmids and Viral Preps - May 2021

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    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

11. Genome-wide Screening Using CRISPR

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    ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

12. Simplify Cloning with in vivo Assembly

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    ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

13. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

15. Great Results Start with Great Standard Curves

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    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

16. Plasmids 101: Protein tags

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    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

17. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ... regulated by guanosine [corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death...

18. Save Time with Transient Plant Leaf Transformations

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    ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

19. Designing Your Chalk Talk for the Academic Job Interview

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    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...